Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection
Background and Objectives: Staphylococcus aureus is the main Gram-positive bacteria isolated from patients with ocular infections. Herein, we describe the pattern of antibiotic resistance, presence of resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl cytotoxin gene in S. aureus iso...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-10-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/1579 |
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| author | Ashkan Faridi Amir Tavakoli Kareshk Mehdi Fatahi-Bafghi Mahsa Ziasistani Mohammad Reza Kandehkar Ghahraman Seyedeh Zeinab Seyyed-Yousefi Noshin Shakeri Davood Kalantar-Neyestanaki |
| author_facet | Ashkan Faridi Amir Tavakoli Kareshk Mehdi Fatahi-Bafghi Mahsa Ziasistani Mohammad Reza Kandehkar Ghahraman Seyedeh Zeinab Seyyed-Yousefi Noshin Shakeri Davood Kalantar-Neyestanaki |
| author_sort | Ashkan Faridi |
| collection | DOAJ |
| description | Background and Objectives: Staphylococcus aureus is the main Gram-positive bacteria isolated from patients with ocular infections. Herein, we describe the pattern of antibiotic resistance, presence of resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl cytotoxin gene in S. aureus isolates collected from patients with external ocular infection.
Materials and Methods: In this study, 8 S. aureus isolates were collected from 81 patients that suffered from eye damage. Antibacterial susceptibility of isolates was determined using the Kirby-Bauer disk diffusion method. Resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl virulence gene were detected by PCR method. Staphylococcal cassette chromosome mec (SCCmec) in MRSA isolates were detected by the multiplex-PCR method.
Results: Three isolates were resistant to cefoxitin which is considered MRSA. The mecA gene was identified in MRSA isolates. SCCmec type IV and the pvl gene were detected in one of the MRSA isolates that was recovered from a diabetic patient.
Conclusion: The emergence of S. aureus isolates belonging to SCCmec type IV and pvl gene among patients with ocular infection is very serious; therefore, identify genetic characterization of MRSA isolates for empirical therapy and infection control is very important. |
| first_indexed | 2024-12-19T12:31:50Z |
| format | Article |
| id | doaj.art-6b2bd2821172412fbe5da25567e15b57 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-19T12:31:50Z |
| publishDate | 2018-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-6b2bd2821172412fbe5da25567e15b572022-12-21T20:21:19ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472018-10-01104Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infectionAshkan Faridi0Amir Tavakoli Kareshk1Mehdi Fatahi-Bafghi2Mahsa Ziasistani3Mohammad Reza Kandehkar Ghahraman4Seyedeh Zeinab Seyyed-Yousefi5Noshin Shakeri6Davood Kalantar-Neyestanaki7Student Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, IranInfectious Diseases Reesearch Center, Birjand University of Medical Sciences, Birjand, IranDepartment of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences Yazd, IranPathology and Stem Cell Research Center, School of Medicine, Kerman University of Medical Sciences, Kerman, IranStudent Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranShahid Labafi Nejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IranBackground and Objectives: Staphylococcus aureus is the main Gram-positive bacteria isolated from patients with ocular infections. Herein, we describe the pattern of antibiotic resistance, presence of resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl cytotoxin gene in S. aureus isolates collected from patients with external ocular infection. Materials and Methods: In this study, 8 S. aureus isolates were collected from 81 patients that suffered from eye damage. Antibacterial susceptibility of isolates was determined using the Kirby-Bauer disk diffusion method. Resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl virulence gene were detected by PCR method. Staphylococcal cassette chromosome mec (SCCmec) in MRSA isolates were detected by the multiplex-PCR method. Results: Three isolates were resistant to cefoxitin which is considered MRSA. The mecA gene was identified in MRSA isolates. SCCmec type IV and the pvl gene were detected in one of the MRSA isolates that was recovered from a diabetic patient. Conclusion: The emergence of S. aureus isolates belonging to SCCmec type IV and pvl gene among patients with ocular infection is very serious; therefore, identify genetic characterization of MRSA isolates for empirical therapy and infection control is very important.https://ijm.tums.ac.ir/index.php/ijm/article/view/1579Eye infectionMethicillin resistance in Staphylococcus aureusPanton–Valentine leukocidin |
| spellingShingle | Ashkan Faridi Amir Tavakoli Kareshk Mehdi Fatahi-Bafghi Mahsa Ziasistani Mohammad Reza Kandehkar Ghahraman Seyedeh Zeinab Seyyed-Yousefi Noshin Shakeri Davood Kalantar-Neyestanaki Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection Iranian Journal of Microbiology Eye infection Methicillin resistance in Staphylococcus aureus Panton–Valentine leukocidin |
| title | Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection |
| title_full | Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection |
| title_fullStr | Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection |
| title_full_unstemmed | Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection |
| title_short | Detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical samples of patients with external ocular infection |
| title_sort | detection of methicillin resistant staphylococcus aureus mrsa in clinical samples of patients with external ocular infection |
| topic | Eye infection Methicillin resistance in Staphylococcus aureus Panton–Valentine leukocidin |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/1579 |
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