Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application
In Korea and China, ilaprazole is a widely used proton pump inhibitor in the treatment of gastric ulcers. In this study, a specific and sensitive LC-MS/MS method has been developed and validated for the quantification of ilaprazole enantiomers in the rat plasma, using R-lansoprazole as the internal...
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| Format: | Article |
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Elsevier
2020-12-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177919304642 |
| _version_ | 1819179933839130624 |
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| author | Fengting Ou Ying Zhou Jinxiu Lei Su Zeng Fuhai Wu Ning Zhang Lushan Yu |
| author_facet | Fengting Ou Ying Zhou Jinxiu Lei Su Zeng Fuhai Wu Ning Zhang Lushan Yu |
| author_sort | Fengting Ou |
| collection | DOAJ |
| description | In Korea and China, ilaprazole is a widely used proton pump inhibitor in the treatment of gastric ulcers. In this study, a specific and sensitive LC-MS/MS method has been developed and validated for the quantification of ilaprazole enantiomers in the rat plasma, using R-lansoprazole as the internal standard. The enantioseparation was achieved on a CHIRALPAK AS-RH column (4.6 mm × 150 mm, i.d. 5 μm), with a mobile phase composed of 10 mM ammonium acetate aqueous solution and acetonitrile (60:40, V/V), at a flow-rate of 0.5 mL/min. The method was validated over the concentration range of 0.5–300 ng/mL for both, R- and S -ilaprazole. The lower limit of quantification was 0.5 ng/mL for both enantiomers. The relative standard deviation (RSD) of intra- and inter-day precision of R-ilaprazole and S-ilaprazole was less than 10.9%, and the relative error accuracy (RE) ranged from −0.5%–2.0%. Finally, the method was successfully evaluated in rats in a stereoselective pharmacokinetic study of the ilaprazole racemate. |
| first_indexed | 2024-12-22T22:06:19Z |
| format | Article |
| id | doaj.art-6b2ee1935e2c406288ecc538763ac9c6 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-22T22:06:19Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-6b2ee1935e2c406288ecc538763ac9c62022-12-21T18:10:58ZengElsevierJournal of Pharmaceutical Analysis2095-17792020-12-01106617623Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic applicationFengting Ou0Ying Zhou1Jinxiu Lei2Su Zeng3Fuhai Wu4Ning Zhang5Lushan Yu6Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China; School of Pharmacy, Guangdong Pharmaceutical University, Guangdong, 510006, ChinaInstitute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, ChinaInstitute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, ChinaInstitute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, ChinaSchool of Public Health, Guangdong Pharmaceutical University, Guangdong, 510006, ChinaInstitute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, 310058, China; Corresponding author.Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China; Corresponding author.In Korea and China, ilaprazole is a widely used proton pump inhibitor in the treatment of gastric ulcers. In this study, a specific and sensitive LC-MS/MS method has been developed and validated for the quantification of ilaprazole enantiomers in the rat plasma, using R-lansoprazole as the internal standard. The enantioseparation was achieved on a CHIRALPAK AS-RH column (4.6 mm × 150 mm, i.d. 5 μm), with a mobile phase composed of 10 mM ammonium acetate aqueous solution and acetonitrile (60:40, V/V), at a flow-rate of 0.5 mL/min. The method was validated over the concentration range of 0.5–300 ng/mL for both, R- and S -ilaprazole. The lower limit of quantification was 0.5 ng/mL for both enantiomers. The relative standard deviation (RSD) of intra- and inter-day precision of R-ilaprazole and S-ilaprazole was less than 10.9%, and the relative error accuracy (RE) ranged from −0.5%–2.0%. Finally, the method was successfully evaluated in rats in a stereoselective pharmacokinetic study of the ilaprazole racemate.http://www.sciencedirect.com/science/article/pii/S2095177919304642IlaprazoleEnantiomerLC-MS/MSPharmacokinetics |
| spellingShingle | Fengting Ou Ying Zhou Jinxiu Lei Su Zeng Fuhai Wu Ning Zhang Lushan Yu Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application Journal of Pharmaceutical Analysis Ilaprazole Enantiomer LC-MS/MS Pharmacokinetics |
| title | Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application |
| title_full | Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application |
| title_fullStr | Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application |
| title_full_unstemmed | Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application |
| title_short | Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application |
| title_sort | development of a uhplc ms ms method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application |
| topic | Ilaprazole Enantiomer LC-MS/MS Pharmacokinetics |
| url | http://www.sciencedirect.com/science/article/pii/S2095177919304642 |
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