The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses
Abstract Background N 6 -methyladenosine (m6A) is the most common RNA modification in eukaryotes and has been implicated as a novel epigenetic marker that is involved in various biological processes. The pattern and functional dissection of m6A in the regulation of several major human viral diseases...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Genome Biology |
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| Online Access: | https://doi.org/10.1186/s13059-021-02410-2 |
| _version_ | 1819063137751531520 |
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| author | Kun Zhang Xinjian Zhuang Zhuozhuo Dong Kai Xu Xijun Chen Fang Liu Zhen He |
| author_facet | Kun Zhang Xinjian Zhuang Zhuozhuo Dong Kai Xu Xijun Chen Fang Liu Zhen He |
| author_sort | Kun Zhang |
| collection | DOAJ |
| description | Abstract Background N 6 -methyladenosine (m6A) is the most common RNA modification in eukaryotes and has been implicated as a novel epigenetic marker that is involved in various biological processes. The pattern and functional dissection of m6A in the regulation of several major human viral diseases have already been reported. However, the patterns and functions of m6A distribution in plant disease bursting remain largely unknown. Results We analyse the high-quality m6A methylomes in rice plants infected with two devastating viruses. We find that the m6A methylation is mainly associated with genes that are not actively expressed in virus-infected rice plants. We also detect different m6A peak distributions on the same gene, which may contribute to different antiviral modes between rice stripe virus or rice black-stripe dwarf virus infection. Interestingly, we observe increased levels of m6A methylation in rice plant response to virus infection. Several antiviral pathway-related genes, such as RNA silencing-, resistance-, and fundamental antiviral phytohormone metabolic-related genes, are also m6A methylated. The level of m6A methylation is tightly associated with its relative expression levels. Conclusions We revealed the dynamics of m6A modification during the interaction between rice and viruses, which may act as a main regulatory strategy in gene expression. Our investigations highlight the significance of m6A modifications in interactions between plant and viruses, especially in regulating the expression of genes involved in key pathways. |
| first_indexed | 2024-12-21T15:09:54Z |
| format | Article |
| id | doaj.art-6b330c98577b4ac7bee49d050349dae8 |
| institution | Directory Open Access Journal |
| issn | 1474-760X |
| language | English |
| last_indexed | 2024-12-21T15:09:54Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj.art-6b330c98577b4ac7bee49d050349dae82022-12-21T18:59:19ZengBMCGenome Biology1474-760X2021-06-0122113610.1186/s13059-021-02410-2The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant virusesKun Zhang0Xinjian Zhuang1Zhuozhuo Dong2Kai Xu3Xijun Chen4Fang Liu5Zhen He6Department of Plant Protection, College of Horticulture and Plant Protection, Yangzhou UniversityDepartment of Plant Protection, College of Horticulture and Plant Protection, Yangzhou UniversityDepartment of Plant Protection, College of Horticulture and Plant Protection, Yangzhou UniversityJiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal UniversityDepartment of Plant Protection, College of Horticulture and Plant Protection, Yangzhou UniversityDepartment of Plant Protection, College of Horticulture and Plant Protection, Yangzhou UniversityDepartment of Plant Protection, College of Horticulture and Plant Protection, Yangzhou UniversityAbstract Background N 6 -methyladenosine (m6A) is the most common RNA modification in eukaryotes and has been implicated as a novel epigenetic marker that is involved in various biological processes. The pattern and functional dissection of m6A in the regulation of several major human viral diseases have already been reported. However, the patterns and functions of m6A distribution in plant disease bursting remain largely unknown. Results We analyse the high-quality m6A methylomes in rice plants infected with two devastating viruses. We find that the m6A methylation is mainly associated with genes that are not actively expressed in virus-infected rice plants. We also detect different m6A peak distributions on the same gene, which may contribute to different antiviral modes between rice stripe virus or rice black-stripe dwarf virus infection. Interestingly, we observe increased levels of m6A methylation in rice plant response to virus infection. Several antiviral pathway-related genes, such as RNA silencing-, resistance-, and fundamental antiviral phytohormone metabolic-related genes, are also m6A methylated. The level of m6A methylation is tightly associated with its relative expression levels. Conclusions We revealed the dynamics of m6A modification during the interaction between rice and viruses, which may act as a main regulatory strategy in gene expression. Our investigations highlight the significance of m6A modifications in interactions between plant and viruses, especially in regulating the expression of genes involved in key pathways.https://doi.org/10.1186/s13059-021-02410-2N 6 -methyladenosineRicePlant virusesInteractions |
| spellingShingle | Kun Zhang Xinjian Zhuang Zhuozhuo Dong Kai Xu Xijun Chen Fang Liu Zhen He The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses Genome Biology N 6 -methyladenosine Rice Plant viruses Interactions |
| title | The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses |
| title_full | The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses |
| title_fullStr | The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses |
| title_full_unstemmed | The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses |
| title_short | The dynamics of N 6-methyladenine RNA modification in interactions between rice and plant viruses |
| title_sort | dynamics of n 6 methyladenine rna modification in interactions between rice and plant viruses |
| topic | N 6 -methyladenosine Rice Plant viruses Interactions |
| url | https://doi.org/10.1186/s13059-021-02410-2 |
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