Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs

<p>Abstract</p> <p>Background</p> <p>Whitefly-transmitted geminiviruses (begomoviruses) are a major limiting factor for the production of numerous dicotyledonous crops throughout the world. Begomoviruses differ in the number of components that make up their genomes and...

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Main Authors: Amin Imran, Patil Basavaprabhu L, Briddon Rob W, Mansoor Shahid, Fauquet Claude M
Format: Article
Language:English
Published: BMC 2011-05-01
Series:Virology Journal
Online Access:http://www.virologyj.com/content/8/1/238
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author Amin Imran
Patil Basavaprabhu L
Briddon Rob W
Mansoor Shahid
Fauquet Claude M
author_facet Amin Imran
Patil Basavaprabhu L
Briddon Rob W
Mansoor Shahid
Fauquet Claude M
author_sort Amin Imran
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>Whitefly-transmitted geminiviruses (begomoviruses) are a major limiting factor for the production of numerous dicotyledonous crops throughout the world. Begomoviruses differ in the number of components that make up their genomes and association with satellites, and yet they cause strikingly similar phenotypes, such as leaf curling, chlorosis and stunted plant growth. MicroRNAs (miRNAs) are small endogenous RNAs that regulate plant growth and development. The study described here was aimed at investigating the effects of each virus encoded gene on the levels of developmental miRNAs to identify common trends between distinct begomoviruses.</p> <p>Results</p> <p>All genes encoded by four distinct begomoviruses (<it>African cassava mosaic virus </it>[ACMV], <it>Cabbage leaf curl virus </it>[CbLCuV], <it>Tomato yellow leaf curl virus </it>[TYLCV] and <it>Cotton leaf curl virus</it>/Cotton leaf curl betasatellite [CLCuV/CLCuMB]) were expressed from a <it>Potato virus X </it>(PVX) vector in <it>Nicotiana benthamiana</it>. Changes in the levels of ten miRNAs in response to the virus genes were determined by northern blotting using specific miRNA probes. For the monopartite begomoviruses (TYLCV and CLCuMV) the V2 gene product was identified as the major symptom determinant while for bipartite begomoviruses (ACMV and CbLCuV) more than one gene appears to contribute to symptoms and this is reflected in changes in miRNA levels. The phenotype induced by expression of the βC1 gene of the betasatellite CLCuMB was the most distinct and consisted of leaf curling, vein swelling, thick green veins and enations and the pattern of changes in miRNA levels was the most distinct.</p> <p>Conclusions</p> <p>Our results have identified symptom determinants encoded by begomoviruses and show that developmental abnormalities caused by transient expression of begomovirus genes correlates with altered levels of developmental miRNAs. Additionally, all begomovirus genes were shown to modulate miRNA levels, the first time this has been shown to be the case.</p>
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spelling doaj.art-6b338d1ecafd4a94acb75824a8862f682022-12-22T02:04:09ZengBMCVirology Journal1743-422X2011-05-018123810.1186/1743-422X-8-238Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAsAmin ImranPatil Basavaprabhu LBriddon Rob WMansoor ShahidFauquet Claude M<p>Abstract</p> <p>Background</p> <p>Whitefly-transmitted geminiviruses (begomoviruses) are a major limiting factor for the production of numerous dicotyledonous crops throughout the world. Begomoviruses differ in the number of components that make up their genomes and association with satellites, and yet they cause strikingly similar phenotypes, such as leaf curling, chlorosis and stunted plant growth. MicroRNAs (miRNAs) are small endogenous RNAs that regulate plant growth and development. The study described here was aimed at investigating the effects of each virus encoded gene on the levels of developmental miRNAs to identify common trends between distinct begomoviruses.</p> <p>Results</p> <p>All genes encoded by four distinct begomoviruses (<it>African cassava mosaic virus </it>[ACMV], <it>Cabbage leaf curl virus </it>[CbLCuV], <it>Tomato yellow leaf curl virus </it>[TYLCV] and <it>Cotton leaf curl virus</it>/Cotton leaf curl betasatellite [CLCuV/CLCuMB]) were expressed from a <it>Potato virus X </it>(PVX) vector in <it>Nicotiana benthamiana</it>. Changes in the levels of ten miRNAs in response to the virus genes were determined by northern blotting using specific miRNA probes. For the monopartite begomoviruses (TYLCV and CLCuMV) the V2 gene product was identified as the major symptom determinant while for bipartite begomoviruses (ACMV and CbLCuV) more than one gene appears to contribute to symptoms and this is reflected in changes in miRNA levels. The phenotype induced by expression of the βC1 gene of the betasatellite CLCuMB was the most distinct and consisted of leaf curling, vein swelling, thick green veins and enations and the pattern of changes in miRNA levels was the most distinct.</p> <p>Conclusions</p> <p>Our results have identified symptom determinants encoded by begomoviruses and show that developmental abnormalities caused by transient expression of begomovirus genes correlates with altered levels of developmental miRNAs. Additionally, all begomovirus genes were shown to modulate miRNA levels, the first time this has been shown to be the case.</p>http://www.virologyj.com/content/8/1/238
spellingShingle Amin Imran
Patil Basavaprabhu L
Briddon Rob W
Mansoor Shahid
Fauquet Claude M
Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs
Virology Journal
title Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs
title_full Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs
title_fullStr Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs
title_full_unstemmed Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs
title_short Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in <it>Nicotiana benthamiana </it>and their correlation with the levels of developmental miRNAs
title_sort comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in it nicotiana benthamiana it and their correlation with the levels of developmental mirnas
url http://www.virologyj.com/content/8/1/238
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