Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria
The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural hosts. Since the detection of severe acute respiratory syndrome coronavirus (SARS-CoV), it is evident that bats are vital reservoirs of coronaviruses (CoVs). In this study, we investigated the presence of Co...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/9/1017 |
| _version_ | 1827658033848647680 |
|---|---|
| author | Uwem George Oluwadamilola George Arthur Oragwa Babatunde Motayo Joshua Kamani Andrew Adamu Oluyomi Sowemimo Richard Adeleke Samson Abalaka Nuhu Sani Judith Oguzie Philomena Eromon Onikepe Folarin Anise Happi Isaac Komolafe Christian Happi |
| author_facet | Uwem George Oluwadamilola George Arthur Oragwa Babatunde Motayo Joshua Kamani Andrew Adamu Oluyomi Sowemimo Richard Adeleke Samson Abalaka Nuhu Sani Judith Oguzie Philomena Eromon Onikepe Folarin Anise Happi Isaac Komolafe Christian Happi |
| author_sort | Uwem George |
| collection | DOAJ |
| description | The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural hosts. Since the detection of severe acute respiratory syndrome coronavirus (SARS-CoV), it is evident that bats are vital reservoirs of coronaviruses (CoVs). In this study, we investigated the presence of CoVs in multiple bat species in Nigeria to identify viruses in bats at high-risk human contact interfaces. Four hundred and nine bats comprising four bat species close to human habitats were individually sampled from five states in Nigeria between 2019 and 2021. Coronavirus detection was done using broadly reactive consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene of CoVs. Coronavirus RNA was detected in 39 samples (9.5%, CI 95%: [7.0, 12.8]), of which 29 were successfully sequenced. The identified CoVs in Nigerian bats were from the unclassified African alphacoronavirus lineage and betacoronavirus lineage D (<i>Nobecovirus</i>), with one sample from <i>Hipposideros ruber</i> coinfected with alphacoronavirus and betacoronavirus. Different bat species roosting in similar or other places had CoVs from the same genetic lineage. The phylogenetic and evolutionary dynamics data indicated a high CoV diversity in Nigeria, while host switching may have contributed to CoV evolution. Robust sentinel surveillance is recommended to enhance our knowledge of emerging and re-emerging coronaviruses. |
| first_indexed | 2024-03-09T22:53:14Z |
| format | Article |
| id | doaj.art-6b33e22fd66d430fa6967551ebb78d85 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:53:14Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-6b33e22fd66d430fa6967551ebb78d852023-11-23T18:16:03ZengMDPI AGPathogens2076-08172022-09-01119101710.3390/pathogens11091017Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in NigeriaUwem George0Oluwadamilola George1Arthur Oragwa2Babatunde Motayo3Joshua Kamani4Andrew Adamu5Oluyomi Sowemimo6Richard Adeleke7Samson Abalaka8Nuhu Sani9Judith Oguzie10Philomena Eromon11Onikepe Folarin12Anise Happi13Isaac Komolafe14Christian Happi15African Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede 232102, Osun State, NigeriaIbadan Diagnostic and Epidemiology Laboratory, National Veterinary Research Institute, Mokola, Ibadan 200212, Oyo State, NigeriaDepartment of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Jos, Jos 930003, Plateau State, NigeriaDepartment of Medical Microbiology, Federal Medical Centre, Abeokuta 110222, Ogun State, NigeriaParasitology Division, National Veterinary Research Institute (NVRI), PMB 01, Vom 930103, Plateau State, NigeriaAustralian Institute of Tropical Health and Medicine, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, AustraliaDepartment of Zoology, Faculty of Science, Obafemi Awolowo University, Ile Ife 220005, Osun State, NigeriaImmunology and Infectious Diseases, College of Veterinary Medicine, Cornell University, New York, NY 14853, USADepartment of Veterinary Pathology, Faculty of Veterinary Medicine, University of Abuja, Abuja 900105, Federal Capital Territory, NigeriaDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, University of Abuja, Abuja 900105, Federal Capital Territory, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede 232102, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede 232102, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede 232102, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede 232102, Osun State, NigeriaDepartment of Biological Sciences, Faculty of Natural Sciences, Redeemer’s University, Ede 232102, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede 232102, Osun State, NigeriaThe rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural hosts. Since the detection of severe acute respiratory syndrome coronavirus (SARS-CoV), it is evident that bats are vital reservoirs of coronaviruses (CoVs). In this study, we investigated the presence of CoVs in multiple bat species in Nigeria to identify viruses in bats at high-risk human contact interfaces. Four hundred and nine bats comprising four bat species close to human habitats were individually sampled from five states in Nigeria between 2019 and 2021. Coronavirus detection was done using broadly reactive consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene of CoVs. Coronavirus RNA was detected in 39 samples (9.5%, CI 95%: [7.0, 12.8]), of which 29 were successfully sequenced. The identified CoVs in Nigerian bats were from the unclassified African alphacoronavirus lineage and betacoronavirus lineage D (<i>Nobecovirus</i>), with one sample from <i>Hipposideros ruber</i> coinfected with alphacoronavirus and betacoronavirus. Different bat species roosting in similar or other places had CoVs from the same genetic lineage. The phylogenetic and evolutionary dynamics data indicated a high CoV diversity in Nigeria, while host switching may have contributed to CoV evolution. Robust sentinel surveillance is recommended to enhance our knowledge of emerging and re-emerging coronaviruses.https://www.mdpi.com/2076-0817/11/9/1017coronavirus diversitybatszoonosissurveillanceNigeria |
| spellingShingle | Uwem George Oluwadamilola George Arthur Oragwa Babatunde Motayo Joshua Kamani Andrew Adamu Oluyomi Sowemimo Richard Adeleke Samson Abalaka Nuhu Sani Judith Oguzie Philomena Eromon Onikepe Folarin Anise Happi Isaac Komolafe Christian Happi Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria Pathogens coronavirus diversity bats zoonosis surveillance Nigeria |
| title | Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria |
| title_full | Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria |
| title_fullStr | Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria |
| title_full_unstemmed | Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria |
| title_short | Detection of Alpha- and Betacoronaviruses in Frugivorous and Insectivorous Bats in Nigeria |
| title_sort | detection of alpha and betacoronaviruses in frugivorous and insectivorous bats in nigeria |
| topic | coronavirus diversity bats zoonosis surveillance Nigeria |
| url | https://www.mdpi.com/2076-0817/11/9/1017 |
| work_keys_str_mv | AT uwemgeorge detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT oluwadamilolageorge detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT arthuroragwa detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT babatundemotayo detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT joshuakamani detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT andrewadamu detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT oluyomisowemimo detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT richardadeleke detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT samsonabalaka detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT nuhusani detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT judithoguzie detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT philomenaeromon detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT onikepefolarin detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT anisehappi detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT isaackomolafe detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria AT christianhappi detectionofalphaandbetacoronavirusesinfrugivorousandinsectivorousbatsinnigeria |