An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia
This study aimed to investigate changes in postmortem urotensin II receptor (UTR) levels in brain and kidney tissues in a rat model of cardiac ischemia. The rats were divided into two groups: a control group and a cardiac ischemia-induced group. Cardiac ischemia was created by an intraperitoneal inj...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Forensic Science and Medicine |
| Subjects: | |
| Online Access: | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2018;volume=4;issue=1;spage=1;epage=6;aulast=Sener |
| _version_ | 1818538338072657920 |
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| author | Mustafa Talip Sener Erol Akpinar Elif Cadirci Zekai Halici Irfan Cinar Ahmet Nezih Kok |
| author_facet | Mustafa Talip Sener Erol Akpinar Elif Cadirci Zekai Halici Irfan Cinar Ahmet Nezih Kok |
| author_sort | Mustafa Talip Sener |
| collection | DOAJ |
| description | This study aimed to investigate changes in postmortem urotensin II receptor (UTR) levels in brain and kidney tissues in a rat model of cardiac ischemia. The rats were divided into two groups: a control group and a cardiac ischemia-induced group. Cardiac ischemia was created by an intraperitoneal injection of a single lethal dose of isoproterenol (ISO; 850 mg/kg). Plasma UT, blood urea nitrogen, and creatinine levels were determined 0 h postmortem. Brain and kidney UTR mRNA expression levels were determined 0, 1, 3, 6, 12, 24, 48, and 72 h postmortem. The histopathological appearance of brain and kidney tissues was also evaluated. Plasma UT and plasma creatinine levels were increased in the cardiac ischemia-induced group as compared with those in the control group (P < 0.001). Ischemia resulted in histopathological changes in brain and cerebellum tissue. The morphological evaluation revealed Purkinje cell degeneration (P = 0.037) and dark neurons (P = 0.004). The UTR expression level decreased after 1 h postmortem in the brain and after 3 h postmortem in the kidneys in the cardiac ischemia-induced group as compared with that in the control group (P < 0.001). The observed changes in UTR expression levels may be valuable in clinical practice in the field of forensic medicine. These changes may be used as a marker in postmortem evaluations of sudden death caused by ischemia-induced cardiac shock. |
| first_indexed | 2024-12-11T21:27:43Z |
| format | Article |
| id | doaj.art-6b3bf075fd2d40ea839c81373b9e2551 |
| institution | Directory Open Access Journal |
| issn | 2349-5014 |
| language | English |
| last_indexed | 2024-12-11T21:27:43Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Forensic Science and Medicine |
| spelling | doaj.art-6b3bf075fd2d40ea839c81373b9e25512022-12-22T00:50:16ZengWolters Kluwer Medknow PublicationsJournal of Forensic Science and Medicine2349-50142018-01-01411610.4103/jfsm.jfsm_75_17An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemiaMustafa Talip SenerErol AkpinarElif CadirciZekai HaliciIrfan CinarAhmet Nezih KokThis study aimed to investigate changes in postmortem urotensin II receptor (UTR) levels in brain and kidney tissues in a rat model of cardiac ischemia. The rats were divided into two groups: a control group and a cardiac ischemia-induced group. Cardiac ischemia was created by an intraperitoneal injection of a single lethal dose of isoproterenol (ISO; 850 mg/kg). Plasma UT, blood urea nitrogen, and creatinine levels were determined 0 h postmortem. Brain and kidney UTR mRNA expression levels were determined 0, 1, 3, 6, 12, 24, 48, and 72 h postmortem. The histopathological appearance of brain and kidney tissues was also evaluated. Plasma UT and plasma creatinine levels were increased in the cardiac ischemia-induced group as compared with those in the control group (P < 0.001). Ischemia resulted in histopathological changes in brain and cerebellum tissue. The morphological evaluation revealed Purkinje cell degeneration (P = 0.037) and dark neurons (P = 0.004). The UTR expression level decreased after 1 h postmortem in the brain and after 3 h postmortem in the kidneys in the cardiac ischemia-induced group as compared with that in the control group (P < 0.001). The observed changes in UTR expression levels may be valuable in clinical practice in the field of forensic medicine. These changes may be used as a marker in postmortem evaluations of sudden death caused by ischemia-induced cardiac shock.http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2018;volume=4;issue=1;spage=1;epage=6;aulast=SenerBraincardiac ischemiakidneypostmortemurotensin II receptor expression |
| spellingShingle | Mustafa Talip Sener Erol Akpinar Elif Cadirci Zekai Halici Irfan Cinar Ahmet Nezih Kok An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia Journal of Forensic Science and Medicine Brain cardiac ischemia kidney postmortem urotensin II receptor expression |
| title | An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia |
| title_full | An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia |
| title_fullStr | An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia |
| title_full_unstemmed | An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia |
| title_short | An investigation of postmortem urotensin II receptor levels in brain and kidney tissues in a rat model of cardiac ischemia |
| title_sort | investigation of postmortem urotensin ii receptor levels in brain and kidney tissues in a rat model of cardiac ischemia |
| topic | Brain cardiac ischemia kidney postmortem urotensin II receptor expression |
| url | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2018;volume=4;issue=1;spage=1;epage=6;aulast=Sener |
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