Establishing Cell Lines from Fresh or Cryopreserved Tissue from the Great Crested Newt (<i>Triturus cristatus</i>): A Preliminary Protocol

Establishing Cell Lines from Fresh or Cryopreserved Tissue from the Great Crested Newt (<i>Triturus cristatus</i>): A Preliminary Protocol

This study describes a successful protocol for establishing cell lines from the threatened <i>Triturus cristatus</i> in terms of collection, preparing, establishing, cryopreserving, thawing and quality checking. Different parameters such as media, media change, fresh vs. cryopreserved ti...

Full description

Bibliographic Details
Main Authors:	Julie Strand, Henrik Callesen, Cino Pertoldi, Stig Purup
Format:	Article
Language:	English
Published:	MDPI AG 2021-02-01
Series:	Animals
Subjects:	cell culture morphology culture conditions xCELLigence<sup>®</sup> real-time cell analysis amphibian
Online Access:	https://www.mdpi.com/2076-2615/11/2/367

Similar Items

Impact of Carbon Fluoroxide Nanoparticles on Cell Proliferation
by: Alain Géloën, et al.
Published: (2021-11-01)

Hec1/Nek2 Mitotic Pathway Inhibitor INH1 Inhibits the Cell Kinetic Parameters of A549 and HeLa Cells
by: İdil Çetin
Published: (2022-01-01)

Analysis of NK-92 cytotoxicity in nasopharyngeal carcinoma cell lines and patient-derived xenografts using impedance-based growth method
by: Fatin Nur Asyiqin Abd Talib, et al.
Published: (2023-07-01)

Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time
by: Dan Kho, et al.
Published: (2015-04-01)

Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells
by: Ayşe Eken, et al.
Published: (2017-11-01)

In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics
by: Maria Luz Prieto, et al.
Published: (2014-04-01)

Real-time monitoring of Pseudomonas aeruginosa biofilm growth dynamics and persister cells’ eradication
by: Miglė Žiemytė, et al.
Published: (2021-01-01)

Comparison of Leading Biosensor Technologies to Detect Changes in Human Endothelial Barrier Properties in Response to Pro-Inflammatory TNFα and IL1β in Real-Time
by: James J. W. Hucklesby, et al.
Published: (2021-05-01)

An improved habitat suitability index for the great crested newt Triturus cristatus
by: Seccombe, E, et al.
Published: (2024)

Migration strategy of the Great crested newt (Triturus cristatus) in an artificial pond
by: Lukáš Weber, et al.
Published: (2023-12-01)

γδ T cell-mediated antibody-dependent cellular cytotoxicity with CD19 antibodies assessed by an impedance-based label-free real-time cytotoxicity assay
by: Ursula Jördis Eva Seidel, et al.
Published: (2014-12-01)

The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (<i>Triturus</i> spp.)
by: Tamara G. Petrović, et al.
Published: (2020-04-01)

Curcumin Enhances the Efficacy of 5-FU in Colo205 Cell Lines
by: Ebru Öztürk, et al.
Published: (2017-11-01)

A real-time assay for neutrophil chemotaxis
by: Patricia M. Cano, et al.
Published: (2016-05-01)

Personalized antibiotic selection in periodontal treatment improves clinical and microbiological outputs
by: Miglė Žiemytė, et al.
Published: (2023-12-01)

Comparing trap and bait efficiency to record the great crested newts (Triturus cristatus)
by: Weber Lukáš, et al.
Published: (2023-01-01)

Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts
by: Alain Géloën, et al.
Published: (2025-01-01)

Melanoma Mediated Disruption of Brain Endothelial Barrier Integrity Is Not Prevented by the Inhibition of Matrix Metalloproteinases and Proteases
by: Akshata Anchan, et al.
Published: (2022-08-01)

Real-time monitoring of biofilm growth identifies andrographolide as a potent antifungal compound eradicating Candida biofilms
by: Miglė Žiemytė, et al.
Published: (2023-12-01)

Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication
by: Guojun Yan, et al.
Published: (2018-08-01)

HMOX1 is partly responsible for phenotypic and functional abnormalities in mesenchymal stem cells/stromal cells from placenta of preeclampsia (PE) patients
by: Yasser S. Basmaeil, et al.
Published: (2020-01-01)

A Screening Study Identified Decitabine as an Inhibitor of Equid Herpesvirus 4 That Enhances the Innate Antiviral Response
by: Camille Normand, et al.
Published: (2024-05-01)

Unraveling the rapid radiation of crested newts (<it>Triturus cristatus </it>superspecies) using complete mitogenomic sequences
by: Wielstra Ben, et al.
Published: (2011-06-01)

Testing the applicability of tagging the Great crested newt (Triturus cristatus) using passive integrated transponders.
by: Lukáš Weber, et al.
Published: (2019-01-01)

Lactobacilli spp.: real-time evaluation of biofilm growth
by: Stacy Martinez, et al.
Published: (2020-03-01)

Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method
by: Michał Miłek, et al.
Published: (2019-03-01)

Defining Evolutionary Conservation Units in the Macedonian Crested Newt, <i>Triturus macedonicus</i> (Amphibia; Salamandridae), in a Biodiversity Hotspot
by: Taxiarchis Danelis, et al.
Published: (2023-05-01)

Viability of developmental stages of Schistosoma mansoni quantified with xCELLigence worm real-time motility assay (xWORM)
by: Gabriel Rinaldi, et al.
Published: (2015-12-01)

Assessing the Use of Artificial Hibernacula by the Great Crested Newt (Triturus cristatus) and Smooth Newt (Lissotriton vulgaris) in Cold Climate in Southeast Norway
by: Børre K. Dervo, et al.
Published: (2018-07-01)

An experimental assessment of detection dog ability to locate great crested newts (Triturus cristatus) at distance and through soil.
by: Nicola Jayne Glover, et al.
Published: (2023-01-01)

Continuously Monitoring the Cytotoxicity of API-1, α-Chaconine and α-Solanine on Human Lung Carcinoma A549
by: Ebru Öztürk, et al.
Published: (2017-11-01)

Malformations and body injuries in a hybrid zone of crested newts (Caudata: Salamandridae: <em>Triturus cristatus</em> superspecies)
by: Zdeněk Mačát, et al.
Published: (2015-12-01)

Australian Scorpion <i>Hormurus waigiensis</i> Venom Fractions Show Broad Bioactivity through Modulation of Bio-Impedance and Cytosolic Calcium
by: David M. Housley, et al.
Published: (2020-04-01)

Label-free detection of prostaglandin transporter (SLCO2A1) function and inhibition: insights by wound healing and TRACT assays
by: Tamara A. M. Mocking, et al.
Published: (2024-05-01)

Species‐specific habitat preferences do not shape the structure of a crested newt hybrid zone (Triturus cristatus x T. carnifex)
by: Zdeněk Mačát, et al.
Published: (2019-11-01)

Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary (<i>Pecari tajacu</i>) Semen after Cooling Storage in a Transport Container at 5 °C
by: Romário P. Santos, et al.
Published: (2024-03-01)

Application of <sup>12</sup>C<sup>6</sup> Heavy Ion-Irradiated BHK-21 Cells in Production of Foot-and-Mouth Disease Vaccine
by: Xiangdong Song, et al.
Published: (2025-02-01)

Feeding ecology of two newt species (Triturus cristatus and Lissotriton vulgaris) during the reproduction season
by: Roşca I., et al.
Published: (2013-04-01)

Effect of Essential Oils on the Oxyntopeptic Cells and Somatostatin and Ghrelin Immunoreactive Cells in the European Sea Bass (<i>Dicentrarchus labrax</i>) Gastric Mucosa
by: Maurizio Mazzoni, et al.
Published: (2021-11-01)

Radical Scavenging Is Not Involved in Thymoquinone-Induced Cell Protection in Neural Oxidative Stress Models
by: Christopher Krewenka, et al.
Published: (2023-04-01)