Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles
Biomimetic nanoparticles hold great promise for photonic-mediated nanomedicine due to the association of the biological functionality of the membrane with the physical/chemical goals of organic/inorganic structures, but studies involving fluorescent biomimetic vesicles are still scarce. The purpose...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/2/444 |
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| author | Júlia Muniz Barcelos Tácio Gonçalves Hayasaki Ricardo Costa de Santana Eliana Martins Lima Sebastião Antonio Mendanha Andris Figueiroa Bakuzis |
| author_facet | Júlia Muniz Barcelos Tácio Gonçalves Hayasaki Ricardo Costa de Santana Eliana Martins Lima Sebastião Antonio Mendanha Andris Figueiroa Bakuzis |
| author_sort | Júlia Muniz Barcelos |
| collection | DOAJ |
| description | Biomimetic nanoparticles hold great promise for photonic-mediated nanomedicine due to the association of the biological functionality of the membrane with the physical/chemical goals of organic/inorganic structures, but studies involving fluorescent biomimetic vesicles are still scarce. The purpose of this article is to determine how photothermal therapy (PTT) with theranostic IR-780-based nanoparticles depends on the dye content, cholesterol content, lipid bilayer phase and cell membrane type. The photophysical responses of synthetic liposomes, cell membrane vesicles and hybrid nanoparticles are compared. The samples were characterized by nanoparticle tracking analysis, photoluminescence, electron spin resonance, and photothermal- and heat-mediated drug release experiments, among other techniques. The photothermal conversion efficiency (PCE) was determined using Roper’s method. All samples excited at 804 nm showed three fluorescence bands, two of them independent of the IR-780 content. Samples with a fluorescence band at around 850 nm showed photobleaching (PBL). Quenching was higher in cell membrane vesicles, while cholesterol inhibited quenching in synthetic liposomes with low dye content. PTT depended on the cell membrane and was more efficient for melanoma than erythrocyte vesicles. Synthetic liposomes containing cholesterol and a high amount of IR-780 presented superior performance in PTT experiments, with a 2.4-fold PCE increase in comparison with free IR-780, no PBL and the ability to heat-trigger doxorubicin release. |
| first_indexed | 2024-03-11T08:16:58Z |
| format | Article |
| id | doaj.art-6b42e1ca0d994efc90f13309057d56b6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T08:16:58Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-6b42e1ca0d994efc90f13309057d56b62023-11-16T22:40:00ZengMDPI AGPharmaceutics1999-49232023-01-0115244410.3390/pharmaceutics15020444Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic VesiclesJúlia Muniz Barcelos0Tácio Gonçalves Hayasaki1Ricardo Costa de Santana2Eliana Martins Lima3Sebastião Antonio Mendanha4Andris Figueiroa Bakuzis5Institute of Physics, Federal University of Goiás, Goiânia 74690-900, GO, BrazilFarmatec, School of Pharmacy, Federal University of Goiás, Goiânia 74690-631, GO, BrazilInstitute of Physics, Federal University of Goiás, Goiânia 74690-900, GO, BrazilFarmatec, School of Pharmacy, Federal University of Goiás, Goiânia 74690-631, GO, BrazilInstitute of Physics, Federal University of Goiás, Goiânia 74690-900, GO, BrazilInstitute of Physics, Federal University of Goiás, Goiânia 74690-900, GO, BrazilBiomimetic nanoparticles hold great promise for photonic-mediated nanomedicine due to the association of the biological functionality of the membrane with the physical/chemical goals of organic/inorganic structures, but studies involving fluorescent biomimetic vesicles are still scarce. The purpose of this article is to determine how photothermal therapy (PTT) with theranostic IR-780-based nanoparticles depends on the dye content, cholesterol content, lipid bilayer phase and cell membrane type. The photophysical responses of synthetic liposomes, cell membrane vesicles and hybrid nanoparticles are compared. The samples were characterized by nanoparticle tracking analysis, photoluminescence, electron spin resonance, and photothermal- and heat-mediated drug release experiments, among other techniques. The photothermal conversion efficiency (PCE) was determined using Roper’s method. All samples excited at 804 nm showed three fluorescence bands, two of them independent of the IR-780 content. Samples with a fluorescence band at around 850 nm showed photobleaching (PBL). Quenching was higher in cell membrane vesicles, while cholesterol inhibited quenching in synthetic liposomes with low dye content. PTT depended on the cell membrane and was more efficient for melanoma than erythrocyte vesicles. Synthetic liposomes containing cholesterol and a high amount of IR-780 presented superior performance in PTT experiments, with a 2.4-fold PCE increase in comparison with free IR-780, no PBL and the ability to heat-trigger doxorubicin release.https://www.mdpi.com/1999-4923/15/2/444cancerheat-triggered drug releasenear-infrared dyephotoluminescencedoxorubicin |
| spellingShingle | Júlia Muniz Barcelos Tácio Gonçalves Hayasaki Ricardo Costa de Santana Eliana Martins Lima Sebastião Antonio Mendanha Andris Figueiroa Bakuzis Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles Pharmaceutics cancer heat-triggered drug release near-infrared dye photoluminescence doxorubicin |
| title | Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles |
| title_full | Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles |
| title_fullStr | Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles |
| title_full_unstemmed | Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles |
| title_short | Photothermal Properties of IR-780-Based Nanoparticles Depend on Nanocarrier Design: A Comparative Study on Synthetic Liposomes and Cell Membrane and Hybrid Biomimetic Vesicles |
| title_sort | photothermal properties of ir 780 based nanoparticles depend on nanocarrier design a comparative study on synthetic liposomes and cell membrane and hybrid biomimetic vesicles |
| topic | cancer heat-triggered drug release near-infrared dye photoluminescence doxorubicin |
| url | https://www.mdpi.com/1999-4923/15/2/444 |
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