RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM

Aim. The purpose of this study was to determine the effect of the pH of metabolites in the digestive system on the growth activity of lactic acid bacteria isolated from the gastrointestinal tract of rabbits. Methods. The paper used 11 strains of lactic acid bacteria isolated from the gastrointestina...

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Main Authors: Ю. М. Похилько, Н. О. Кравченко
Format: Article
Language:English
Published: Odessa I. I. Mechnikov National University 2017-07-01
Series:Mìkrobìologìâ ì Bìotehnologìâ
Subjects:
Online Access:http://mbt.onu.edu.ua/article/view/105019
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author Ю. М. Похилько
Н. О. Кравченко
author_facet Ю. М. Похилько
Н. О. Кравченко
author_sort Ю. М. Похилько
collection DOAJ
description Aim. The purpose of this study was to determine the effect of the pH of metabolites in the digestive system on the growth activity of lactic acid bacteria isolated from the gastrointestinal tract of rabbits. Methods. The paper used 11 strains of lactic acid bacteria isolated from the gastrointestinal tract of rabbits. Stability of bacteria to the metabolites of the digestive system was determined by culturing them in MRS medium with bile, hydrogen chloride, sodium chloride, phenol. Results. It was found that the strains studied survived at a pH of 4.0 to 9.0. All the strains had high resistance to bile concentration (20, 40%), hydrogen chloride (3%), sodium chloride (5%), phenol (0.5%), viable cells - 103–105 CFU / ml after 48 hours cultivation. Conclusions. It was found that the studied strains of lactobacilli were resistant to high concentrations of bile and phenol, 70% to low pH, 50% to sodium chloride, and 20% to hydrogen chloride.
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spelling doaj.art-6b437ba9a90847eb879e5fe3f5d94dc72022-12-21T20:17:28ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632017-07-0102(38)10111110.18524/2307-4663.2017.2(38).105019105019RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEMЮ. М. Похилько0Н. О. Кравченко1Інститут сільськогосподарської мікробіології та агропромислового виробництва НААНІнститут сільськогосподарської мікробіології та агропромислового виробництва НААНAim. The purpose of this study was to determine the effect of the pH of metabolites in the digestive system on the growth activity of lactic acid bacteria isolated from the gastrointestinal tract of rabbits. Methods. The paper used 11 strains of lactic acid bacteria isolated from the gastrointestinal tract of rabbits. Stability of bacteria to the metabolites of the digestive system was determined by culturing them in MRS medium with bile, hydrogen chloride, sodium chloride, phenol. Results. It was found that the strains studied survived at a pH of 4.0 to 9.0. All the strains had high resistance to bile concentration (20, 40%), hydrogen chloride (3%), sodium chloride (5%), phenol (0.5%), viable cells - 103–105 CFU / ml after 48 hours cultivation. Conclusions. It was found that the studied strains of lactobacilli were resistant to high concentrations of bile and phenol, 70% to low pH, 50% to sodium chloride, and 20% to hydrogen chloride.http://mbt.onu.edu.ua/article/view/105019молочнокислі бактеріїстійкістьгідроген хлориджовчнатрій хлоридфенол
spellingShingle Ю. М. Похилько
Н. О. Кравченко
RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM
Mìkrobìologìâ ì Bìotehnologìâ
молочнокислі бактерії
стійкість
гідроген хлорид
жовч
натрій хлорид
фенол
title RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM
title_full RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM
title_fullStr RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM
title_full_unstemmed RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM
title_short RESISTANCE OF BACTERIA OF THE GENUS LACTOBACILLUS TO THE METABOLITES OF THE DIGESTIVE SYSTEM
title_sort resistance of bacteria of the genus lactobacillus to the metabolites of the digestive system
topic молочнокислі бактерії
стійкість
гідроген хлорид
жовч
натрій хлорид
фенол
url http://mbt.onu.edu.ua/article/view/105019
work_keys_str_mv AT ûmpohilʹko resistanceofbacteriaofthegenuslactobacillustothemetabolitesofthedigestivesystem
AT nokravčenko resistanceofbacteriaofthegenuslactobacillustothemetabolitesofthedigestivesystem