Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α
Immunotherapy in cancer patients is a very promising treatment and the development of new protocols and the study of the mechanisms of regression is imperative. The objective of this study was to evaluate the production of cytokines in helper T (CD4+) lymphocytes during immunotherapy with pegylated...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/16/3/5497 |
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| author | Márcia Antoniazi Michelin Letícia Montes Rosekeila Simões Nomelini Marco Aurélio Trovó Eddie Fernando Candido Murta |
| author_facet | Márcia Antoniazi Michelin Letícia Montes Rosekeila Simões Nomelini Marco Aurélio Trovó Eddie Fernando Candido Murta |
| author_sort | Márcia Antoniazi Michelin |
| collection | DOAJ |
| description | Immunotherapy in cancer patients is a very promising treatment and the development of new protocols and the study of the mechanisms of regression is imperative. The objective of this study was to evaluate the production of cytokines in helper T (CD4+) lymphocytes during immunotherapy with pegylated IFN-α in patients with cervical intraepithelial neoplasia (CIN). We conducted a prospective study with 17 patients with CIN II-III using immunotherapy with pegylated IFN-α subcutaneouly weekly, and using flow cytometry we evaluated the peripheric CD4+ T lymphocytes. The results show that in the regression group the patients presented a significant increase in the amount of IFN-γ during the entire immunotherapy, compared with the group without a response. The amount of CD4+ T lymphocytes positive for IL-2, IL-4, IL-10 and TGF-β is significantly lower in patients with good clinical response. The results also demonstrate that patients with regression have a higher amount of intracellular TNF-α in CD4+ T lymphocytes before the start of treatment. Analyzing these data sets, it can be concluded that immunotherapy is a viable clinical treatment for patients with high-grade CIN and that the regression is dependent on the change in the immune response to a Th1 pattern. |
| first_indexed | 2024-04-12T09:57:30Z |
| format | Article |
| id | doaj.art-6b43c8f5be05485093176f0e85cd241c |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T09:57:30Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6b43c8f5be05485093176f0e85cd241c2022-12-22T03:37:38ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-03-011635497550910.3390/ijms16035497ijms16035497Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-αMárcia Antoniazi Michelin0Letícia Montes1Rosekeila Simões Nomelini2Marco Aurélio Trovó3Eddie Fernando Candido Murta4Oncology Research Institute (IPON), Federal University of the TriânguloMineiro (UFTM), Uberaba, Minas Gerais 38022-200, BrazilOncology Research Institute (IPON), Federal University of the TriânguloMineiro (UFTM), Uberaba, Minas Gerais 38022-200, BrazilDiscipline of Gynecology and Obstetrics, UFTM, Uberaba, Minas Gerais 38022-200, BrazilDiscipline of Gynecology and Obstetrics, UFTM, Uberaba, Minas Gerais 38022-200, BrazilOncology Research Institute (IPON), Federal University of the TriânguloMineiro (UFTM), Uberaba, Minas Gerais 38022-200, BrazilImmunotherapy in cancer patients is a very promising treatment and the development of new protocols and the study of the mechanisms of regression is imperative. The objective of this study was to evaluate the production of cytokines in helper T (CD4+) lymphocytes during immunotherapy with pegylated IFN-α in patients with cervical intraepithelial neoplasia (CIN). We conducted a prospective study with 17 patients with CIN II-III using immunotherapy with pegylated IFN-α subcutaneouly weekly, and using flow cytometry we evaluated the peripheric CD4+ T lymphocytes. The results show that in the regression group the patients presented a significant increase in the amount of IFN-γ during the entire immunotherapy, compared with the group without a response. The amount of CD4+ T lymphocytes positive for IL-2, IL-4, IL-10 and TGF-β is significantly lower in patients with good clinical response. The results also demonstrate that patients with regression have a higher amount of intracellular TNF-α in CD4+ T lymphocytes before the start of treatment. Analyzing these data sets, it can be concluded that immunotherapy is a viable clinical treatment for patients with high-grade CIN and that the regression is dependent on the change in the immune response to a Th1 pattern.http://www.mdpi.com/1422-0067/16/3/5497immunotherapycervical cancerIFN-α and T lymphocytes |
| spellingShingle | Márcia Antoniazi Michelin Letícia Montes Rosekeila Simões Nomelini Marco Aurélio Trovó Eddie Fernando Candido Murta Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α International Journal of Molecular Sciences immunotherapy cervical cancer IFN-α and T lymphocytes |
| title | Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α |
| title_full | Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α |
| title_fullStr | Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α |
| title_full_unstemmed | Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α |
| title_short | Helper T Lymphocyte Response in the Peripheral Blood of Patients with Intraepithelial Neoplasia Submitted to Immunotherapy with Pegylated Interferon-α |
| title_sort | helper t lymphocyte response in the peripheral blood of patients with intraepithelial neoplasia submitted to immunotherapy with pegylated interferon α |
| topic | immunotherapy cervical cancer IFN-α and T lymphocytes |
| url | http://www.mdpi.com/1422-0067/16/3/5497 |
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