Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens
Chile has promoted the diversification of aquaculture and red cusk-eel (<i>Genypterus chilensis</i>) is one of the prioritized species. However, many aspects of the biology of the species are unknown or have little information available. These include intestinal microbiota, an element th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/1/105 |
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| author | Jaime Romero Osmán Díaz Claudio D. Miranda Rodrigo Rojas |
| author_facet | Jaime Romero Osmán Díaz Claudio D. Miranda Rodrigo Rojas |
| author_sort | Jaime Romero |
| collection | DOAJ |
| description | Chile has promoted the diversification of aquaculture and red cusk-eel (<i>Genypterus chilensis</i>) is one of the prioritized species. However, many aspects of the biology of the species are unknown or have little information available. These include intestinal microbiota, an element that may play an important role in the nutrition and defense of cultured animals for meat production. This study compares the microbiota composition of the intestinal contents of wild and aquaculture fish to explore the microbial communities present and their potential contribution to the host. DNA was extracted from the intestinal content samples and the V4 region of the 16S rRNA gene was amplified and sequenced using the Ion Torrent platform. After the examination of the sequences, strong differences were found in the composition at the level of phylum, being <i>Firmicutes</i> and <i>Tenericutes</i> the most abundant in aquaculture and wild condition, respectively. At the genus level, the <i>Vagococcus</i> (54%) and <i>Mycoplasma</i> (97%) were the most prevalent in the microbial community of aquaculture and wild condition, respectively. The evaluation of predicted metabolic pathways in these metagenomes showed that in wild condition there is an important presence of lipid metabolism belonging to the unsaturated fatty acid synthesis. In the aquaculture condition, the metabolism of terpenoids and polyketides were relevant. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of red cusk-eel (<i>Genypterus chilensis</i>) of wild and aquaculture origin using high-throughput sequencing. |
| first_indexed | 2024-03-10T00:53:50Z |
| format | Article |
| id | doaj.art-6b44e05cf6514ea4aae45cb723dd686a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T00:53:50Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6b44e05cf6514ea4aae45cb723dd686a2023-11-23T14:47:25ZengMDPI AGMicroorganisms2076-26072022-01-0110110510.3390/microorganisms10010105Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture SpecimensJaime Romero0Osmán Díaz1Claudio D. Miranda2Rodrigo Rojas3Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Líbano 5524, Macul, Santiago 783090, ChileInstituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Líbano 5524, Macul, Santiago 783090, ChileLaboratorio de Patobiología Acuática, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo 1780000, ChileLaboratorio de Patobiología Acuática, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo 1780000, ChileChile has promoted the diversification of aquaculture and red cusk-eel (<i>Genypterus chilensis</i>) is one of the prioritized species. However, many aspects of the biology of the species are unknown or have little information available. These include intestinal microbiota, an element that may play an important role in the nutrition and defense of cultured animals for meat production. This study compares the microbiota composition of the intestinal contents of wild and aquaculture fish to explore the microbial communities present and their potential contribution to the host. DNA was extracted from the intestinal content samples and the V4 region of the 16S rRNA gene was amplified and sequenced using the Ion Torrent platform. After the examination of the sequences, strong differences were found in the composition at the level of phylum, being <i>Firmicutes</i> and <i>Tenericutes</i> the most abundant in aquaculture and wild condition, respectively. At the genus level, the <i>Vagococcus</i> (54%) and <i>Mycoplasma</i> (97%) were the most prevalent in the microbial community of aquaculture and wild condition, respectively. The evaluation of predicted metabolic pathways in these metagenomes showed that in wild condition there is an important presence of lipid metabolism belonging to the unsaturated fatty acid synthesis. In the aquaculture condition, the metabolism of terpenoids and polyketides were relevant. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of red cusk-eel (<i>Genypterus chilensis</i>) of wild and aquaculture origin using high-throughput sequencing.https://www.mdpi.com/2076-2607/10/1/105microbiotamicrobiome<i>Genypterus chilensis</i><i>Tenericutes</i>Ophidiidaefish |
| spellingShingle | Jaime Romero Osmán Díaz Claudio D. Miranda Rodrigo Rojas Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens Microorganisms microbiota microbiome <i>Genypterus chilensis</i> <i>Tenericutes</i> Ophidiidae fish |
| title | Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens |
| title_full | Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens |
| title_fullStr | Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens |
| title_full_unstemmed | Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens |
| title_short | Red Cusk-Eel (<i>Genypterus chilensis</i>) Gut Microbiota Description of Wild and Aquaculture Specimens |
| title_sort | red cusk eel i genypterus chilensis i gut microbiota description of wild and aquaculture specimens |
| topic | microbiota microbiome <i>Genypterus chilensis</i> <i>Tenericutes</i> Ophidiidae fish |
| url | https://www.mdpi.com/2076-2607/10/1/105 |
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