New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice
<i>Escherichia coli</i> is responsible for cases of diarrhea around the world, and some studies have shown the benefits of cinnamaldehyde in the treatment of bacterial disease. Therefore, the objective of this study was to evaluate the effects of cinnamaldehyde in mice colonized by patho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/2/302 |
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| author | Wellison A. Pereira Carlos Drielson S. Pereira Raíssa G. Assunção Iandeyara Savanna C. da Silva Fabrícia S. Rego Leylane S. R. Alves Juliana S. Santos Francisco Jonathas R. Nogueira Adrielle Zagmignan Thomas T. Thomsen Anders Løbner-Olesen Karen A. Krogfelt Luís Cláudio N. da Silva Afonso G. Abreu |
| author_facet | Wellison A. Pereira Carlos Drielson S. Pereira Raíssa G. Assunção Iandeyara Savanna C. da Silva Fabrícia S. Rego Leylane S. R. Alves Juliana S. Santos Francisco Jonathas R. Nogueira Adrielle Zagmignan Thomas T. Thomsen Anders Løbner-Olesen Karen A. Krogfelt Luís Cláudio N. da Silva Afonso G. Abreu |
| author_sort | Wellison A. Pereira |
| collection | DOAJ |
| description | <i>Escherichia coli</i> is responsible for cases of diarrhea around the world, and some studies have shown the benefits of cinnamaldehyde in the treatment of bacterial disease. Therefore, the objective of this study was to evaluate the effects of cinnamaldehyde in mice colonized by pathogenic <i>E. coli</i>, as well as to provide more insights into its antimicrobial action mechanism. After determination of minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations, the interference of cinnamaldehyde in macromolecular pathways (synthesis of DNA, RNA, protein, and cell wall) was measured by incorporation of radioisotopes. The anti-adhesive properties of cinnamaldehyde towards <i>E. coli</i> 042 were evaluated using human epithelial type 2 (HEp-2) cells. Intestinal colonization was tested on mice, and the effect of cinnamaldehyde on <i>Tenebrio molitor</i> larvae. Cinnamaldehyde showed MIC and MBC values of 780 μg/mL and 1560 μg/mL, respectively; reduced the adhesion of <i>E. coli</i> 042 on HEp-2 cells; and affected all the synthetic pathways evaluated, suggesting that compost impairs the membrane/cell wall structure leading bacteria to total collapse. No effect on the expression of genes related to the SOS pathway (<i>sulA</i> and <i>dinB1</i>) was observed. The compound did not interfere with cell viability and was not toxic against <i>T. molitor</i> larvae. In addition, cinnamaldehyde-treated mice exhibited lower levels of colonization by <i>E. coli</i> 042 than the untreated group. Therefore, the results show that cinnamaldehyde is effective in treating the pathogenic <i>E. coli</i> strain 042 and confirm it as a promising lead molecule for the development of antimicrobial agents. |
| first_indexed | 2024-03-09T00:47:39Z |
| format | Article |
| id | doaj.art-6b463eb01e07427ca1be6e3db5af7115 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T00:47:39Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6b463eb01e07427ca1be6e3db5af71152023-12-11T17:26:27ZengMDPI AGBiomolecules2218-273X2021-02-0111230210.3390/biom11020302New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of MiceWellison A. Pereira0Carlos Drielson S. Pereira1Raíssa G. Assunção2Iandeyara Savanna C. da Silva3Fabrícia S. Rego4Leylane S. R. Alves5Juliana S. Santos6Francisco Jonathas R. Nogueira7Adrielle Zagmignan8Thomas T. Thomsen9Anders Løbner-Olesen10Karen A. Krogfelt11Luís Cláudio N. da Silva12Afonso G. Abreu13Laboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilDepartment of Functional Genomics, University of Copenhagen, 2200 Copenhagen, DenmarkDepartment of Functional Genomics, University of Copenhagen, 2200 Copenhagen, DenmarkDepartment of Science and Environment, Roskilde University, 4000 Roskilde, DenmarkLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil<i>Escherichia coli</i> is responsible for cases of diarrhea around the world, and some studies have shown the benefits of cinnamaldehyde in the treatment of bacterial disease. Therefore, the objective of this study was to evaluate the effects of cinnamaldehyde in mice colonized by pathogenic <i>E. coli</i>, as well as to provide more insights into its antimicrobial action mechanism. After determination of minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations, the interference of cinnamaldehyde in macromolecular pathways (synthesis of DNA, RNA, protein, and cell wall) was measured by incorporation of radioisotopes. The anti-adhesive properties of cinnamaldehyde towards <i>E. coli</i> 042 were evaluated using human epithelial type 2 (HEp-2) cells. Intestinal colonization was tested on mice, and the effect of cinnamaldehyde on <i>Tenebrio molitor</i> larvae. Cinnamaldehyde showed MIC and MBC values of 780 μg/mL and 1560 μg/mL, respectively; reduced the adhesion of <i>E. coli</i> 042 on HEp-2 cells; and affected all the synthetic pathways evaluated, suggesting that compost impairs the membrane/cell wall structure leading bacteria to total collapse. No effect on the expression of genes related to the SOS pathway (<i>sulA</i> and <i>dinB1</i>) was observed. The compound did not interfere with cell viability and was not toxic against <i>T. molitor</i> larvae. In addition, cinnamaldehyde-treated mice exhibited lower levels of colonization by <i>E. coli</i> 042 than the untreated group. Therefore, the results show that cinnamaldehyde is effective in treating the pathogenic <i>E. coli</i> strain 042 and confirm it as a promising lead molecule for the development of antimicrobial agents.https://www.mdpi.com/2218-273X/11/2/302cinnamaldehydeintestinal colonizationnatural products |
| spellingShingle | Wellison A. Pereira Carlos Drielson S. Pereira Raíssa G. Assunção Iandeyara Savanna C. da Silva Fabrícia S. Rego Leylane S. R. Alves Juliana S. Santos Francisco Jonathas R. Nogueira Adrielle Zagmignan Thomas T. Thomsen Anders Løbner-Olesen Karen A. Krogfelt Luís Cláudio N. da Silva Afonso G. Abreu New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice Biomolecules cinnamaldehyde intestinal colonization natural products |
| title | New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice |
| title_full | New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice |
| title_fullStr | New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice |
| title_full_unstemmed | New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice |
| title_short | New Insights into the Antimicrobial Action of Cinnamaldehyde towards <i>Escherichia coli</i> and Its Effects on Intestinal Colonization of Mice |
| title_sort | new insights into the antimicrobial action of cinnamaldehyde towards i escherichia coli i and its effects on intestinal colonization of mice |
| topic | cinnamaldehyde intestinal colonization natural products |
| url | https://www.mdpi.com/2218-273X/11/2/302 |
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