Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes
Background: Monocyte-derived macrophages are key regulators and producers of reactive oxygen and nitrogen species (ROS/RNS). Pre-clinical and clinical studies suggest that cold physical plasma may be beneficial in the treatment of inflammatory conditions via the release of ROS/RNS. However, it is un...
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MDPI AG
2019-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/12/2530 |
| _version_ | 1818409427995197440 |
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| author | Eric Freund Juliane Moritz Matthias Stope Christian Seebauer Anke Schmidt Sander Bekeschus |
| author_facet | Eric Freund Juliane Moritz Matthias Stope Christian Seebauer Anke Schmidt Sander Bekeschus |
| author_sort | Eric Freund |
| collection | DOAJ |
| description | Background: Monocyte-derived macrophages are key regulators and producers of reactive oxygen and nitrogen species (ROS/RNS). Pre-clinical and clinical studies suggest that cold physical plasma may be beneficial in the treatment of inflammatory conditions via the release of ROS/RNS. However, it is unknown how plasma treatment affects monocytes and their differentiation profile. Methods: Naïve or phorbol-12-myristate-13-acetate (PMA)-pulsed THP-1 monocytes were exposed to cold physical plasma. The cells were analyzed regarding their metabolic activity as well as flow cytometry (analysis of viability, oxidation, surface marker expression and cytokine secretion) and high content imaging (quantitative analysis of morphology. Results: The plasma treatment affected THP-1 metabolisms, viability, and morphology. Furthermore, a significant modulation CD55, CD69, CD271 surface-expression and increase of inflammatory IL1β, IL6, IL8, and MCP1 secretion was observed upon plasma treatment. Distinct phenotypical changes in THP-1 cells arguing for a differentiation profile were validated in primary monocytes from donor blood. As a functional outcome, plasma-treated monocytes decreased the viability of co-cultured melanoma cells to a greater extent than their non-treated counterparts. Conclusions: Our results suggest plasma-derived ROS/RNS shaped a differentiation profile in human monocytes as evidenced by their increased inflammatory profile (surface marker and cytokines) as well as functional outcome (tumor toxicity). |
| first_indexed | 2024-12-14T09:59:28Z |
| format | Article |
| id | doaj.art-6b47f94165e8417db33a45cb6acf819b |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-14T09:59:28Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-6b47f94165e8417db33a45cb6acf819b2022-12-21T23:07:19ZengMDPI AGApplied Sciences2076-34172019-06-01912253010.3390/app9122530app9122530Plasma-Derived Reactive Species Shape a Differentiation Profile in Human MonocytesEric Freund0Juliane Moritz1Matthias Stope2Christian Seebauer3Anke Schmidt4Sander Bekeschus5ZIK <i>plasmatis</i>, Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix Hausdorff Str. 2, 17489 Greifswald, GermanyZIK <i>plasmatis</i>, Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix Hausdorff Str. 2, 17489 Greifswald, GermanyDepartment of Urology, University Medicine Greifswald, Ferdinand-Sauerbruch-Str., 17475 Greifswald, GermanyDepartment of Oral and Maxillofacial Surgery, University Medicine Greifswald, Ferdinand-Sauerbruch-Str., 17475 Greifswald, GermanyZIK <i>plasmatis</i>, Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix Hausdorff Str. 2, 17489 Greifswald, GermanyZIK <i>plasmatis</i>, Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix Hausdorff Str. 2, 17489 Greifswald, GermanyBackground: Monocyte-derived macrophages are key regulators and producers of reactive oxygen and nitrogen species (ROS/RNS). Pre-clinical and clinical studies suggest that cold physical plasma may be beneficial in the treatment of inflammatory conditions via the release of ROS/RNS. However, it is unknown how plasma treatment affects monocytes and their differentiation profile. Methods: Naïve or phorbol-12-myristate-13-acetate (PMA)-pulsed THP-1 monocytes were exposed to cold physical plasma. The cells were analyzed regarding their metabolic activity as well as flow cytometry (analysis of viability, oxidation, surface marker expression and cytokine secretion) and high content imaging (quantitative analysis of morphology. Results: The plasma treatment affected THP-1 metabolisms, viability, and morphology. Furthermore, a significant modulation CD55, CD69, CD271 surface-expression and increase of inflammatory IL1β, IL6, IL8, and MCP1 secretion was observed upon plasma treatment. Distinct phenotypical changes in THP-1 cells arguing for a differentiation profile were validated in primary monocytes from donor blood. As a functional outcome, plasma-treated monocytes decreased the viability of co-cultured melanoma cells to a greater extent than their non-treated counterparts. Conclusions: Our results suggest plasma-derived ROS/RNS shaped a differentiation profile in human monocytes as evidenced by their increased inflammatory profile (surface marker and cytokines) as well as functional outcome (tumor toxicity).https://www.mdpi.com/2076-3417/9/12/2530cold physical plasmakINPenmacrophagesRNSROS |
| spellingShingle | Eric Freund Juliane Moritz Matthias Stope Christian Seebauer Anke Schmidt Sander Bekeschus Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes Applied Sciences cold physical plasma kINPen macrophages RNS ROS |
| title | Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes |
| title_full | Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes |
| title_fullStr | Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes |
| title_full_unstemmed | Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes |
| title_short | Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes |
| title_sort | plasma derived reactive species shape a differentiation profile in human monocytes |
| topic | cold physical plasma kINPen macrophages RNS ROS |
| url | https://www.mdpi.com/2076-3417/9/12/2530 |
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