Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles
Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global tempe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02378/full |
| _version_ | 1811192127150882816 |
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| author | Eric Altermann Eric Altermann Linley R. Schofield Ron S. Ronimus Amy K. Beattie Kerri Reilly |
| author_facet | Eric Altermann Eric Altermann Linley R. Schofield Ron S. Ronimus Amy K. Beattie Kerri Reilly |
| author_sort | Eric Altermann |
| collection | DOAJ |
| description | Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global temperature rises. Here we demonstrate the use of a lytic enyzme, PeiR, from a methanogen virus that infects Methanobrevibacter ruminantium M1 as an effective agent inhibiting a range of rumen methanogen strains in pure culture. We determined the substrate specificity of soluble PeiR and demonstrated that the enzyme is capable of hydrolysing the pseudomurein cell walls of methanogens. Subsequently, peiR was fused to the polyhydroxyalkanoate (PHA) synthase gene phaC and displayed on the surface of PHA bionanoparticles (BNPs) expressed in Eschericia coli via one-step biosynthesis. These tailored BNPs were capable of lysing not only the original methanogen host strain, but a wide range of other rumen methanogen strains in vitro. Methane production was reduced by up to 97% for 5 days post-inoculation in the in vitro assay. We propose that tailored BNPs carrying anti-methanogen enzymes represent a new class of methane inhibitors. Tailored BNPs can be rapidly developed and may be able to modulate the methanogen community in vivo with the aim to lower ruminant methane emissions without impacting animal productivity. |
| first_indexed | 2024-04-11T23:46:37Z |
| format | Article |
| id | doaj.art-6b4c7fac70204bd29dba75e6a2939890 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T23:46:37Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6b4c7fac70204bd29dba75e6a29398902022-12-22T03:56:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02378407781Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored BionanoparticlesEric Altermann0Eric Altermann1Linley R. Schofield2Ron S. Ronimus3Amy K. Beattie4Kerri Reilly5Rumen Microbiology, Animal Science, AgResearch Ltd., Palmerston North, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandRumen Microbiology, Animal Science, AgResearch Ltd., Palmerston North, New ZealandRumen Microbiology, Animal Science, AgResearch Ltd., Palmerston North, New ZealandSoil Biology, Forage Science, AgResearch Ltd., Christchurch, New ZealandRumen Microbiology, Animal Science, AgResearch Ltd., Palmerston North, New ZealandMethane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global temperature rises. Here we demonstrate the use of a lytic enyzme, PeiR, from a methanogen virus that infects Methanobrevibacter ruminantium M1 as an effective agent inhibiting a range of rumen methanogen strains in pure culture. We determined the substrate specificity of soluble PeiR and demonstrated that the enzyme is capable of hydrolysing the pseudomurein cell walls of methanogens. Subsequently, peiR was fused to the polyhydroxyalkanoate (PHA) synthase gene phaC and displayed on the surface of PHA bionanoparticles (BNPs) expressed in Eschericia coli via one-step biosynthesis. These tailored BNPs were capable of lysing not only the original methanogen host strain, but a wide range of other rumen methanogen strains in vitro. Methane production was reduced by up to 97% for 5 days post-inoculation in the in vitro assay. We propose that tailored BNPs carrying anti-methanogen enzymes represent a new class of methane inhibitors. Tailored BNPs can be rapidly developed and may be able to modulate the methanogen community in vivo with the aim to lower ruminant methane emissions without impacting animal productivity.https://www.frontiersin.org/article/10.3389/fmicb.2018.02378/fullbionanoparticlesmethane mitigationlytic enzymearchaeamethanogensPHA |
| spellingShingle | Eric Altermann Eric Altermann Linley R. Schofield Ron S. Ronimus Amy K. Beattie Kerri Reilly Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles Frontiers in Microbiology bionanoparticles methane mitigation lytic enzyme archaea methanogens PHA |
| title | Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles |
| title_full | Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles |
| title_fullStr | Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles |
| title_full_unstemmed | Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles |
| title_short | Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles |
| title_sort | inhibition of rumen methanogens by a novel archaeal lytic enzyme displayed on tailored bionanoparticles |
| topic | bionanoparticles methane mitigation lytic enzyme archaea methanogens PHA |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02378/full |
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