Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression
In this study, HIV-1 viral load quantitation determined by Nucleic Acid Sequence Based Amplification (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and b-2 microglobulin) in 540 patients followed up at São Paulo, SP, Brazil. HIV-1 RNA detectio...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1998-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000300027&tlng=en |
| _version_ | 1797757044449607680 |
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| author | Roberta Sitnik João Renato Rebello Pinho |
| author_facet | Roberta Sitnik João Renato Rebello Pinho |
| author_sort | Roberta Sitnik |
| collection | DOAJ |
| description | In this study, HIV-1 viral load quantitation determined by Nucleic Acid Sequence Based Amplification (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and b-2 microglobulin) in 540 patients followed up at São Paulo, SP, Brazil. HIV-1 RNA detection was statistically associated with the presence of antigen p24, but the viral RNA was also detected in 68% of the antigen p24 negative samples, confirming that NASBA is much more sensitive than the determination of antigen p24. Regarding other surrogate markers, no statistically significant association with the detection of viral RNA was found. The reproducibility of this viral load assay was assessed by 14 runs of the same sample, using different reagents batches. Viral load values in this sample ranged from 5.83 to 6.27 log (CV = 36 %), less than the range (0.5 log) established to the determination of significant viral load changes. |
| first_indexed | 2024-03-12T18:09:24Z |
| format | Article |
| id | doaj.art-6b4dcf8907dd488284ddf943b93919b2 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:09:24Z |
| publishDate | 1998-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-6b4dcf8907dd488284ddf943b93919b22023-08-02T09:16:32ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80601998-05-0193341141610.1590/S0074-02761998000300027Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease ProgressionRoberta Sitnik0João Renato Rebello Pinho1Bioquímico Jardim PaulistaInstituto Adolfo LutzIn this study, HIV-1 viral load quantitation determined by Nucleic Acid Sequence Based Amplification (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and b-2 microglobulin) in 540 patients followed up at São Paulo, SP, Brazil. HIV-1 RNA detection was statistically associated with the presence of antigen p24, but the viral RNA was also detected in 68% of the antigen p24 negative samples, confirming that NASBA is much more sensitive than the determination of antigen p24. Regarding other surrogate markers, no statistically significant association with the detection of viral RNA was found. The reproducibility of this viral load assay was assessed by 14 runs of the same sample, using different reagents batches. Viral load values in this sample ranged from 5.83 to 6.27 log (CV = 36 %), less than the range (0.5 log) established to the determination of significant viral load changes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000300027&tlng=enHuman Immunodeficiency Virus type 1viral loadNucleic Acid Sequence Based Amplification (NASBA)surrogate markers |
| spellingShingle | Roberta Sitnik João Renato Rebello Pinho Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression Memorias do Instituto Oswaldo Cruz Human Immunodeficiency Virus type 1 viral load Nucleic Acid Sequence Based Amplification (NASBA) surrogate markers |
| title | Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression |
| title_full | Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression |
| title_fullStr | Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression |
| title_full_unstemmed | Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression |
| title_short | Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression |
| title_sort | quantitation of hiv 1 rna viral load using nasba methodology and comparison with other surrogate markers for disease progression |
| topic | Human Immunodeficiency Virus type 1 viral load Nucleic Acid Sequence Based Amplification (NASBA) surrogate markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000300027&tlng=en |
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