Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’
As a valuable ornamental plant, <i>Sinningia hybrida</i> ‘Isa’s Murmur’ (<i>S. hybrida</i>) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approa...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/9/1232 |
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| author | Honglin Yang Yihua Yang Qiang Wang Jinyu He Liyun Liang Hui Qiu Yue Wang Lijuan Zou |
| author_facet | Honglin Yang Yihua Yang Qiang Wang Jinyu He Liyun Liang Hui Qiu Yue Wang Lijuan Zou |
| author_sort | Honglin Yang |
| collection | DOAJ |
| description | As a valuable ornamental plant, <i>Sinningia hybrida</i> ‘Isa’s Murmur’ (<i>S. hybrida</i>) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approach for the sustainable propagation of <i>S. hybrida</i>. Compared with aseptic seeding leaves explants, field-grown leaves explants are more suitable for adventitious shoot regeneration. Adding 0.1 mg L<sup>−1</sup> NAA and 2.0 mg L<sup>−1</sup> TDZ could obtain the highest adventitious shoot proliferation coefficient (24.5), and the induction rate was 91.7%. The shoot proliferation coefficient (20.7) and the greatest shoot length and induction rate (95.3%) were achieved in 0.1 mg L<sup>−1</sup> NAA and 2.0 mg L<sup>−1</sup> BA medium, accompanied by rooting formation. Adding 0.5 mg L<sup>−1</sup> GA<sub>3</sub>, 1.0 mg L<sup>−1</sup> BA, and 0.2 mg L<sup>−1</sup> IBA to MS medium can effectively prolong the regenerated buds for rooting. The best for rooting was 1/2 MS medium containing 0.3 mg L<sup>−1</sup> IBA, with the maximum number of roots (13.4 per shoot) and survival rate for transplanting (100%). This work aims to build an efficient, definitive, and scalable protocol for <i>S. hybrida</i> regeneration useful for large-scale cultivation and even more protoplast fusion and genetic transformation to develop more colorful or fragrant flowers. |
| first_indexed | 2024-03-10T03:47:52Z |
| format | Article |
| id | doaj.art-6b544240e69a46359d971fbe359cc181 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:47:52Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-6b544240e69a46359d971fbe359cc1812023-11-23T09:03:24ZengMDPI AGPlants2223-77472022-05-01119123210.3390/plants11091232Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’Honglin Yang0Yihua Yang1Qiang Wang2Jinyu He3Liyun Liang4Hui Qiu5Yue Wang6Lijuan Zou7Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest, Sichuan Agricultural University at Wenjiang, Chengdu 611130, ChinaEcological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaCollege of Life Sciences, China West Normal University, Nanchong 637009, ChinaEcological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaEcological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaEcological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaEcological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaAs a valuable ornamental plant, <i>Sinningia hybrida</i> ‘Isa’s Murmur’ (<i>S. hybrida</i>) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approach for the sustainable propagation of <i>S. hybrida</i>. Compared with aseptic seeding leaves explants, field-grown leaves explants are more suitable for adventitious shoot regeneration. Adding 0.1 mg L<sup>−1</sup> NAA and 2.0 mg L<sup>−1</sup> TDZ could obtain the highest adventitious shoot proliferation coefficient (24.5), and the induction rate was 91.7%. The shoot proliferation coefficient (20.7) and the greatest shoot length and induction rate (95.3%) were achieved in 0.1 mg L<sup>−1</sup> NAA and 2.0 mg L<sup>−1</sup> BA medium, accompanied by rooting formation. Adding 0.5 mg L<sup>−1</sup> GA<sub>3</sub>, 1.0 mg L<sup>−1</sup> BA, and 0.2 mg L<sup>−1</sup> IBA to MS medium can effectively prolong the regenerated buds for rooting. The best for rooting was 1/2 MS medium containing 0.3 mg L<sup>−1</sup> IBA, with the maximum number of roots (13.4 per shoot) and survival rate for transplanting (100%). This work aims to build an efficient, definitive, and scalable protocol for <i>S. hybrida</i> regeneration useful for large-scale cultivation and even more protoplast fusion and genetic transformation to develop more colorful or fragrant flowers.https://www.mdpi.com/2223-7747/11/9/1232<i>Sinningia hybrida</i> ‘Isa’s Murmur’leaf explantadventitious shoot regenerationplant growth regulators |
| spellingShingle | Honglin Yang Yihua Yang Qiang Wang Jinyu He Liyun Liang Hui Qiu Yue Wang Lijuan Zou Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’ Plants <i>Sinningia hybrida</i> ‘Isa’s Murmur’ leaf explant adventitious shoot regeneration plant growth regulators |
| title | Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’ |
| title_full | Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’ |
| title_fullStr | Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’ |
| title_full_unstemmed | Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’ |
| title_short | Adventitious Shoot Regeneration from Leaf Explants in <i>Sinningia Hybrida</i> ‘Isa’s Murmur’ |
| title_sort | adventitious shoot regeneration from leaf explants in i sinningia hybrida i isa s murmur |
| topic | <i>Sinningia hybrida</i> ‘Isa’s Murmur’ leaf explant adventitious shoot regeneration plant growth regulators |
| url | https://www.mdpi.com/2223-7747/11/9/1232 |
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