Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch.
FoxP3(+) T cells populate tumors and regulate anti-tumor immunity. The requirement for optimal population of FoxP3(+) regulatory T cells in tumors remains unclear. We investigated the migration requirement and stability of tumor-associated FoxP3(+) T cells. We found that only memory, but not naïve,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3264621?pdf=render |
| _version_ | 1811276336565583872 |
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| author | Chuanwu Wang Jee H Lee Chang H Kim |
| author_facet | Chuanwu Wang Jee H Lee Chang H Kim |
| author_sort | Chuanwu Wang |
| collection | DOAJ |
| description | FoxP3(+) T cells populate tumors and regulate anti-tumor immunity. The requirement for optimal population of FoxP3(+) regulatory T cells in tumors remains unclear. We investigated the migration requirement and stability of tumor-associated FoxP3(+) T cells. We found that only memory, but not naïve, FoxP3(+) T cells are highly enriched in tumors. Almost all of the tumor-infiltrating FoxP3(+) T cells express Helios, an antigen associated either with thymus-generated FoxP3(+) T cells or activated T cells in the periphery. The tumor-infiltrating FoxP3(+) T cells largely lack CD62L and CCR7, two trafficking receptors required for T cell migration into secondary lymphoid tissues. Instead, the tumor infiltrating FoxP3(+) T cells highly express memory/tumor-associated CCR8 and CXCR4. Antigen priming is required for induction of this trafficking receptor phenotype in FoxP3(+) T cells and only antigen primed, but not antigen-inexperienced naive, FoxP3(+) T cells can efficiently migrate into tumors. While the migration of FoxP3(+) T cells into tumors was a readily detectable event, generation of induced FoxP3(+) T cells within tumors was unexpectedly inefficient. Genetic marking of current and ex-FoxP3(+) T cells revealed that tumor-infiltrating FoxP3(+) T cells are highly stable and do not readily convert back to FoxP3(-) T cells. Taken together, our results indicate that population of tumors with thymus-generated FoxP3(+) T cells requires an antigen priming-dependent trafficking receptor switch in lymphoid tissues. |
| first_indexed | 2024-04-12T23:55:46Z |
| format | Article |
| id | doaj.art-6b54b2353573499c90d569305aec757a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T23:55:46Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6b54b2353573499c90d569305aec757a2022-12-22T03:11:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e3079310.1371/journal.pone.0030793Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch.Chuanwu WangJee H LeeChang H KimFoxP3(+) T cells populate tumors and regulate anti-tumor immunity. The requirement for optimal population of FoxP3(+) regulatory T cells in tumors remains unclear. We investigated the migration requirement and stability of tumor-associated FoxP3(+) T cells. We found that only memory, but not naïve, FoxP3(+) T cells are highly enriched in tumors. Almost all of the tumor-infiltrating FoxP3(+) T cells express Helios, an antigen associated either with thymus-generated FoxP3(+) T cells or activated T cells in the periphery. The tumor-infiltrating FoxP3(+) T cells largely lack CD62L and CCR7, two trafficking receptors required for T cell migration into secondary lymphoid tissues. Instead, the tumor infiltrating FoxP3(+) T cells highly express memory/tumor-associated CCR8 and CXCR4. Antigen priming is required for induction of this trafficking receptor phenotype in FoxP3(+) T cells and only antigen primed, but not antigen-inexperienced naive, FoxP3(+) T cells can efficiently migrate into tumors. While the migration of FoxP3(+) T cells into tumors was a readily detectable event, generation of induced FoxP3(+) T cells within tumors was unexpectedly inefficient. Genetic marking of current and ex-FoxP3(+) T cells revealed that tumor-infiltrating FoxP3(+) T cells are highly stable and do not readily convert back to FoxP3(-) T cells. Taken together, our results indicate that population of tumors with thymus-generated FoxP3(+) T cells requires an antigen priming-dependent trafficking receptor switch in lymphoid tissues.http://europepmc.org/articles/PMC3264621?pdf=render |
| spellingShingle | Chuanwu Wang Jee H Lee Chang H Kim Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. PLoS ONE |
| title | Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. |
| title_full | Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. |
| title_fullStr | Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. |
| title_full_unstemmed | Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. |
| title_short | Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch. |
| title_sort | optimal population of foxp3 t cells in tumors requires an antigen priming dependent trafficking receptor switch |
| url | http://europepmc.org/articles/PMC3264621?pdf=render |
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