Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides
Mesenchymal stromal cells (MSCs) are multipotent cells found in several adult tissues; they have the capacity to differentiate into mesodermal, ectodermal, and endodermal tissues in vitro. There have been several reports that MSCs have therapeutic effects in a variety of diseases. Therefore, using a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2007-09-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000007783465271 |
| _version_ | 1811336577937309696 |
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| author | Jae Kwon Lee Man Kyoung Lee Hye Jin Jin Dal-Soo Kim Yoon Sun Yang Wonil Oh Sung-Eun Yang Tae Seok Park Soo Yeol Lee Bum-Soo Kim Sin-Soo Jeun |
| author_facet | Jae Kwon Lee Man Kyoung Lee Hye Jin Jin Dal-Soo Kim Yoon Sun Yang Wonil Oh Sung-Eun Yang Tae Seok Park Soo Yeol Lee Bum-Soo Kim Sin-Soo Jeun |
| author_sort | Jae Kwon Lee |
| collection | DOAJ |
| description | Mesenchymal stromal cells (MSCs) are multipotent cells found in several adult tissues; they have the capacity to differentiate into mesodermal, ectodermal, and endodermal tissues in vitro. There have been several reports that MSCs have therapeutic effects in a variety of diseases. Therefore, using a cell labeling technique, monitoring their temporal and spatial migration in vivo, would be useful in the clinical setting. Magnetic resonance imaging (MRI)—tracking of superparamagnetic iron oxide (SPIO)-labeled cells—is a noninvasive technique for determining the location and migration of transplanted cells. In the present study, we evaluated the influence and toxicity of SPIO (ferumoxides) labeling on multiple differentiated MSCs. To evaluate the influence and toxicity of ferumoxides labeling on differentiation of MSCs, a variety of concentrations of ferumoxides were used for labeling MSCs. We found that the cytoplasm of adherent cells was effectively labeled at low concentrations of ferumoxides. Compared with unlabeled controls, the ferumoxides-labeled MSCs exhibited a similar proliferation rate and apoptotic progression. The labeled MSCs differentiated into osteoblasts and adipocytes in an identical fashion as the unlabeled cells. However, chondrogenesis and neurogenesis were inhibited at high concentrations of ferumoxides. Our results suggest the effective concentration for ferumoxides use in tracking MSCs. |
| first_indexed | 2024-04-13T17:41:26Z |
| format | Article |
| id | doaj.art-6b5833c0aceb45af889df94be810dd85 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-04-13T17:41:26Z |
| publishDate | 2007-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-6b5833c0aceb45af889df94be810dd852022-12-22T02:37:10ZengSAGE PublishingCell Transplantation0963-68971555-38922007-09-011610.3727/000000007783465271Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with FerumoxidesJae Kwon Lee0Man Kyoung Lee1Hye Jin Jin2Dal-Soo Kim3Yoon Sun Yang4Wonil Oh5Sung-Eun Yang6Tae Seok Park7Soo Yeol Lee8Bum-Soo Kim9Sin-Soo Jeun10 School of Science Education (Biology Education), College of Education, Chungbuk National University, Chungcheongbuk-Do, 361-763, Republic of Korea Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea Department of Biomedical Engineering, Kyung Hee University, Seoul, 130-701, Republic of Korea Department of Biomedical Engineering, Kyung Hee University, Seoul, 130-701, Republic of Korea Department of Radiology, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea Department of Neurosurgery, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of KoreaMesenchymal stromal cells (MSCs) are multipotent cells found in several adult tissues; they have the capacity to differentiate into mesodermal, ectodermal, and endodermal tissues in vitro. There have been several reports that MSCs have therapeutic effects in a variety of diseases. Therefore, using a cell labeling technique, monitoring their temporal and spatial migration in vivo, would be useful in the clinical setting. Magnetic resonance imaging (MRI)—tracking of superparamagnetic iron oxide (SPIO)-labeled cells—is a noninvasive technique for determining the location and migration of transplanted cells. In the present study, we evaluated the influence and toxicity of SPIO (ferumoxides) labeling on multiple differentiated MSCs. To evaluate the influence and toxicity of ferumoxides labeling on differentiation of MSCs, a variety of concentrations of ferumoxides were used for labeling MSCs. We found that the cytoplasm of adherent cells was effectively labeled at low concentrations of ferumoxides. Compared with unlabeled controls, the ferumoxides-labeled MSCs exhibited a similar proliferation rate and apoptotic progression. The labeled MSCs differentiated into osteoblasts and adipocytes in an identical fashion as the unlabeled cells. However, chondrogenesis and neurogenesis were inhibited at high concentrations of ferumoxides. Our results suggest the effective concentration for ferumoxides use in tracking MSCs.https://doi.org/10.3727/000000007783465271 |
| spellingShingle | Jae Kwon Lee Man Kyoung Lee Hye Jin Jin Dal-Soo Kim Yoon Sun Yang Wonil Oh Sung-Eun Yang Tae Seok Park Soo Yeol Lee Bum-Soo Kim Sin-Soo Jeun Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides Cell Transplantation |
| title | Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides |
| title_full | Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides |
| title_fullStr | Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides |
| title_full_unstemmed | Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides |
| title_short | Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides |
| title_sort | efficient intracytoplasmic labeling of human umbilical cord blood mesenchymal stromal cells with ferumoxides |
| url | https://doi.org/10.3727/000000007783465271 |
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