A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain
Abstract Background Inbred strains are characterized by less genetic variation, which suggests usefulness of inbred strains for evaluations of various parameters. In this study, experimental reproducibility in several parameters was compared between an outbred Wistar rat and Wistar King A Hokkaido (...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-020-01320-9 |
| _version_ | 1818524088693424128 |
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| author | Tomoko Shimoda Shota Hori Kenta Maegawa Akari Takeuchi Yeonmi Lee Ga-Hyun Joe Yasutake Tanaka Hidehisa Shimizu Satoshi Ishizuka |
| author_facet | Tomoko Shimoda Shota Hori Kenta Maegawa Akari Takeuchi Yeonmi Lee Ga-Hyun Joe Yasutake Tanaka Hidehisa Shimizu Satoshi Ishizuka |
| author_sort | Tomoko Shimoda |
| collection | DOAJ |
| description | Abstract Background Inbred strains are characterized by less genetic variation, which suggests usefulness of inbred strains for evaluations of various parameters. In this study, experimental reproducibility in several parameters was compared between an outbred Wistar rat and Wistar King A Hokkaido (WKAH/HkmSlc) rat, the inbred strain that is originated from Wistar rats. Methods Difference of variations was investigated in parameters of body compositions and liver functions such as body weight, liver weight, liver triglycerides (TG), liver cholesterol and plasma alanine aminotransferase activity (ALT) between WKAH rats and outbred Wistar rats by using the coefficient of variation (CV). Results There was no difference in the CVs of body weight and relative liver weight between WKAH and Wistar rats. The CVs of body weight and relative liver weight were below 10% in both WKAH and Wistar rats. The CVs of TG, cholesterol, and ALT in Wistar rats were between 30 and 40%, whereas those in WKAH rats were between 10 and 25%. A low CV level of TG was observed in WKAH rats compared to that in Wistar rats regardless of the duration of the experimental period in those rat strains. Conclusion The low CV values in metabolic parameters involved in liver functions in the inbred rats suggested an advantage of using inbred rather than outbred rats for the evaluation of liver lipid metabolism. |
| first_indexed | 2024-12-11T05:52:50Z |
| format | Article |
| id | doaj.art-6b5b1ee395774b58b5ec9f71d172fd62 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-11T05:52:50Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-6b5b1ee395774b58b5ec9f71d172fd622022-12-22T01:18:46ZengBMCLipids in Health and Disease1476-511X2020-06-011911410.1186/s12944-020-01320-9A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strainTomoko Shimoda0Shota Hori1Kenta Maegawa2Akari Takeuchi3Yeonmi Lee4Ga-Hyun Joe5Yasutake Tanaka6Hidehisa Shimizu7Satoshi Ishizuka8Research Faculty of Agriculture, Hokkaido UniversityResearch Faculty of Agriculture, Hokkaido UniversityResearch Faculty of Agriculture, Hokkaido UniversityResearch Faculty of Agriculture, Hokkaido UniversityLee Gil Ya Cancer and Diabetes Institute, Gachon UniversityResearch Faculty of Fisheries, Hokkaido UniversityFaculty of Agriculture, Graduate School, Kyushu UniversityInstitute of Agricultural and Life Science, Academic Assembly, Shimane UniversityResearch Faculty of Agriculture, Hokkaido UniversityAbstract Background Inbred strains are characterized by less genetic variation, which suggests usefulness of inbred strains for evaluations of various parameters. In this study, experimental reproducibility in several parameters was compared between an outbred Wistar rat and Wistar King A Hokkaido (WKAH/HkmSlc) rat, the inbred strain that is originated from Wistar rats. Methods Difference of variations was investigated in parameters of body compositions and liver functions such as body weight, liver weight, liver triglycerides (TG), liver cholesterol and plasma alanine aminotransferase activity (ALT) between WKAH rats and outbred Wistar rats by using the coefficient of variation (CV). Results There was no difference in the CVs of body weight and relative liver weight between WKAH and Wistar rats. The CVs of body weight and relative liver weight were below 10% in both WKAH and Wistar rats. The CVs of TG, cholesterol, and ALT in Wistar rats were between 30 and 40%, whereas those in WKAH rats were between 10 and 25%. A low CV level of TG was observed in WKAH rats compared to that in Wistar rats regardless of the duration of the experimental period in those rat strains. Conclusion The low CV values in metabolic parameters involved in liver functions in the inbred rats suggested an advantage of using inbred rather than outbred rats for the evaluation of liver lipid metabolism.http://link.springer.com/article/10.1186/s12944-020-01320-9Coefficient of variationCholesterolInbredLiverTriglycerides |
| spellingShingle | Tomoko Shimoda Shota Hori Kenta Maegawa Akari Takeuchi Yeonmi Lee Ga-Hyun Joe Yasutake Tanaka Hidehisa Shimizu Satoshi Ishizuka A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain Lipids in Health and Disease Coefficient of variation Cholesterol Inbred Liver Triglycerides |
| title | A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain |
| title_full | A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain |
| title_fullStr | A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain |
| title_full_unstemmed | A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain |
| title_short | A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain |
| title_sort | low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain |
| topic | Coefficient of variation Cholesterol Inbred Liver Triglycerides |
| url | http://link.springer.com/article/10.1186/s12944-020-01320-9 |
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