Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027
<i>Enterococcus faecium</i> FUA027 transforms ellagic acid (EA) to urolithin A (UA), which makes it a potential application in the preparation of UA by industrial fermentation. Here, the genetic and probiotic characteristics of <i>E. faecium</i> FUA027 were evaluated through...
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MDPI AG
2023-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/5/1021 |
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| author | Mengjie Xia Shuting Mu Yaowei Fang Xiaomeng Zhang Guang Yang Xiaoyue Hou Fuxiang He Yaling Zhao Yichen Huang Wei Zhang Juan Shen Shu Liu |
| author_facet | Mengjie Xia Shuting Mu Yaowei Fang Xiaomeng Zhang Guang Yang Xiaoyue Hou Fuxiang He Yaling Zhao Yichen Huang Wei Zhang Juan Shen Shu Liu |
| author_sort | Mengjie Xia |
| collection | DOAJ |
| description | <i>Enterococcus faecium</i> FUA027 transforms ellagic acid (EA) to urolithin A (UA), which makes it a potential application in the preparation of UA by industrial fermentation. Here, the genetic and probiotic characteristics of <i>E. faecium</i> FUA027 were evaluated through whole-genome sequence analysis and phenotypic assays. The chromosome size of this strain was 2,718,096 bp, with a GC content of 38.27%. The whole-genome analysis revealed that the genome contained 18 antibiotic resistance genes and seven putative virulence factor genes. <i>E. faecium</i> FUA027 does not contain plasmids and mobile genetic elements (MGEs), and so the transmissibility of antibiotic resistance genes or putative virulence factors should not occur. Phenotypic testing further indicated that <i>E. faecium</i> FUA027 is sensitive to clinically relevant antibiotics. In addition, this bacterium exhibited no hemolytic activity, no biogenic amine production, and could significantly inhibit the growth of the quality control strain. In vitro viability was >60% in all simulated gastrointestinal environments, with good antioxidant activity. The study results suggest that <i>E. faecium</i> FUA027 has the potential to be used in industrial fermentation for the production of urolithin A. |
| first_indexed | 2024-03-11T07:24:30Z |
| format | Article |
| id | doaj.art-6b5eebadd7fc43ff98a6df0f24b72d7c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T07:24:30Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-6b5eebadd7fc43ff98a6df0f24b72d7c2023-11-17T07:41:07ZengMDPI AGFoods2304-81582023-02-01125102110.3390/foods12051021Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027Mengjie Xia0Shuting Mu1Yaowei Fang2Xiaomeng Zhang3Guang Yang4Xiaoyue Hou5Fuxiang He6Yaling Zhao7Yichen Huang8Wei Zhang9Juan Shen10Shu Liu11Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China<i>Enterococcus faecium</i> FUA027 transforms ellagic acid (EA) to urolithin A (UA), which makes it a potential application in the preparation of UA by industrial fermentation. Here, the genetic and probiotic characteristics of <i>E. faecium</i> FUA027 were evaluated through whole-genome sequence analysis and phenotypic assays. The chromosome size of this strain was 2,718,096 bp, with a GC content of 38.27%. The whole-genome analysis revealed that the genome contained 18 antibiotic resistance genes and seven putative virulence factor genes. <i>E. faecium</i> FUA027 does not contain plasmids and mobile genetic elements (MGEs), and so the transmissibility of antibiotic resistance genes or putative virulence factors should not occur. Phenotypic testing further indicated that <i>E. faecium</i> FUA027 is sensitive to clinically relevant antibiotics. In addition, this bacterium exhibited no hemolytic activity, no biogenic amine production, and could significantly inhibit the growth of the quality control strain. In vitro viability was >60% in all simulated gastrointestinal environments, with good antioxidant activity. The study results suggest that <i>E. faecium</i> FUA027 has the potential to be used in industrial fermentation for the production of urolithin A.https://www.mdpi.com/2304-8158/12/5/1021<i>Enterococcus faecium</i>urolithin Asafety assessmentgenomephenotypic assays |
| spellingShingle | Mengjie Xia Shuting Mu Yaowei Fang Xiaomeng Zhang Guang Yang Xiaoyue Hou Fuxiang He Yaling Zhao Yichen Huang Wei Zhang Juan Shen Shu Liu Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027 Foods <i>Enterococcus faecium</i> urolithin A safety assessment genome phenotypic assays |
| title | Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027 |
| title_full | Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027 |
| title_fullStr | Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027 |
| title_full_unstemmed | Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027 |
| title_short | Genetic and Probiotic Characteristics of Urolithin A Producing <i>Enterococcus faecium</i> FUA027 |
| title_sort | genetic and probiotic characteristics of urolithin a producing i enterococcus faecium i fua027 |
| topic | <i>Enterococcus faecium</i> urolithin A safety assessment genome phenotypic assays |
| url | https://www.mdpi.com/2304-8158/12/5/1021 |
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