Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan
Hospital environment and inanimate surfaces are considered as potential sources of opportunistic and nosocomial pathogens. Indirect transmission of microbes from the hospital surfaces has a major role in hospital acquired nosocomial infections and their colonization. The present study was designed...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The National Information and Documentation Centre (NIDOC) , Egypt
2019-12-01
|
| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_66745_e8fb5e58db49264b5ca513b3e2ffd3f1.pdf |
| _version_ | 1797204116494090240 |
|---|---|
| author | Hafsa Zaib Rabia Kanwar Nishat Zafar Sultan Ali |
| author_facet | Hafsa Zaib Rabia Kanwar Nishat Zafar Sultan Ali |
| author_sort | Hafsa Zaib |
| collection | DOAJ |
| description | Hospital environment and inanimate surfaces are considered as potential sources of opportunistic and
nosocomial pathogens. Indirect transmission of microbes from the hospital surfaces has a major role in hospital
acquired nosocomial infections and their colonization. The present study was designed to investigate the
prevalence of major pathogenic bacteria isolated from hospital inanimate surfaces. Random swab samples were
taken from inanimate surfaces and apparatus used in daily treatment of patients from the major hospitals in district
of Faisalabad, Pakistan. These swab samples were cultivated on suitable culture media including; nutrient agar,
MacConkey’s agar and blood agar to isolate the bacterial pathogens. Identification of the bacterial cultures was
carried out by observing the cultural and macroscopic characteristics including Gram staining. Further verification
of these bacterial cultures was carried out using appropriate biochemical assays. The biochemical assays were
performed for characterization of Staphylococcus aureus, Staph. epidermidis, Staph. saprophyticus, Pseudomonas
aeruginosa, Klebsiella pneumonia and Escherichia coli. These pure cultures were then tested for resistance to
different antibiotics using the Kirby–Bauer method. The prevalence of Gram positive bacteria (60.83%) was more
than that of Gram negative bacteria (24.13%). The results of antibiotics susceptibility tests were analyzed
statistically. |
| first_indexed | 2024-04-24T08:30:07Z |
| format | Article |
| id | doaj.art-6b605dbb405a4b4891471322887ad474 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:30:07Z |
| publishDate | 2019-12-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-6b605dbb405a4b4891471322887ad4742024-04-16T20:24:39ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942019-12-013652653410.21608/nrmj.2019.66745Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, PakistanHafsa Zaib0Rabia Kanwar1Nishat Zafar2Sultan Ali3 Institute of Microbiology, Faculty of Veterinary science, University of Agriculture Faisalabad-38000, Punjab, PakistanInstitute of Microbiology, Faculty of Veterinary science, University of Agriculture Faisalabad-38000, Punjab, PakistanInstitute of Microbiology, Faculty of Veterinary science, University of Agriculture Faisalabad-38000, Punjab, PakistanInstitute of Microbiology, Faculty of Veterinary science, University of Agriculture Faisalabad-38000, Punjab, PakistanHospital environment and inanimate surfaces are considered as potential sources of opportunistic and nosocomial pathogens. Indirect transmission of microbes from the hospital surfaces has a major role in hospital acquired nosocomial infections and their colonization. The present study was designed to investigate the prevalence of major pathogenic bacteria isolated from hospital inanimate surfaces. Random swab samples were taken from inanimate surfaces and apparatus used in daily treatment of patients from the major hospitals in district of Faisalabad, Pakistan. These swab samples were cultivated on suitable culture media including; nutrient agar, MacConkey’s agar and blood agar to isolate the bacterial pathogens. Identification of the bacterial cultures was carried out by observing the cultural and macroscopic characteristics including Gram staining. Further verification of these bacterial cultures was carried out using appropriate biochemical assays. The biochemical assays were performed for characterization of Staphylococcus aureus, Staph. epidermidis, Staph. saprophyticus, Pseudomonas aeruginosa, Klebsiella pneumonia and Escherichia coli. These pure cultures were then tested for resistance to different antibiotics using the Kirby–Bauer method. The prevalence of Gram positive bacteria (60.83%) was more than that of Gram negative bacteria (24.13%). The results of antibiotics susceptibility tests were analyzed statistically.https://nrmj.journals.ekb.eg/article_66745_e8fb5e58db49264b5ca513b3e2ffd3f1.pdfbacterial pathogensnosocomial infectioninanimate surfaceskirby-bauer method |
| spellingShingle | Hafsa Zaib Rabia Kanwar Nishat Zafar Sultan Ali Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan Novel Research in Microbiology Journal bacterial pathogens nosocomial infection inanimate surfaces kirby-bauer method |
| title | Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan |
| title_full | Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan |
| title_fullStr | Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan |
| title_full_unstemmed | Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan |
| title_short | Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan |
| title_sort | prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in faisalabad pakistan |
| topic | bacterial pathogens nosocomial infection inanimate surfaces kirby-bauer method |
| url | https://nrmj.journals.ekb.eg/article_66745_e8fb5e58db49264b5ca513b3e2ffd3f1.pdf |
| work_keys_str_mv | AT hafsazaib prevalenceandmultidrugresistanceprofilesofseveralbacterialpathogensisolatedfromhospitalinanimatesurfacesinfaisalabadpakistan AT rabiakanwar prevalenceandmultidrugresistanceprofilesofseveralbacterialpathogensisolatedfromhospitalinanimatesurfacesinfaisalabadpakistan AT nishatzafar prevalenceandmultidrugresistanceprofilesofseveralbacterialpathogensisolatedfromhospitalinanimatesurfacesinfaisalabadpakistan AT sultanali prevalenceandmultidrugresistanceprofilesofseveralbacterialpathogensisolatedfromhospitalinanimatesurfacesinfaisalabadpakistan |