Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation
Spindle orientation is often achieved by a complex of Partner of Inscuteable (Pins)/LGN, Mushroom Body Defect (Mud)/Nuclear Mitotic Apparatus (NuMa), Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this proce...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/78779 |
| _version_ | 1811203636808646656 |
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| author | Jaclyn Camuglia Soline Chanet Adam C Martin |
| author_facet | Jaclyn Camuglia Soline Chanet Adam C Martin |
| author_sort | Jaclyn Camuglia |
| collection | DOAJ |
| description | Spindle orientation is often achieved by a complex of Partner of Inscuteable (Pins)/LGN, Mushroom Body Defect (Mud)/Nuclear Mitotic Apparatus (NuMa), Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this process. Here, we identify Pins-mediated planar cell polarized divisions in several of the mitotic domains of the early Drosophila embryo. We found that neither planar cell polarity pathways nor planar polarized myosin localization determined division orientation; instead, our findings strongly suggest that Pins planar polarity and force generated from mesoderm invagination are important. Disrupting Pins polarity via overexpression of a myristoylated version of Pins caused randomized division angles. We found that disrupting forces through chemical inhibitors, depletion of an adherens junction protein, or blocking mesoderm invagination disrupted Pins planar polarity and spindle orientation. Furthermore, directional ablations that separated mesoderm from mitotic domains disrupted spindle orientation, suggesting that forces transmitted from mesoderm to mitotic domains can polarize Pins and orient division during gastrulation. To our knowledge, this is the first in vivo example where mechanical force has been shown to polarize Pins to mediate division orientation. |
| first_indexed | 2024-04-12T02:58:31Z |
| format | Article |
| id | doaj.art-6b61dd0e81344fb0a3cf1ca8b0cadad1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:58:31Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-6b61dd0e81344fb0a3cf1ca8b0cadad12022-12-22T03:50:43ZengeLife Sciences Publications LtdeLife2050-084X2022-07-011110.7554/eLife.78779Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulationJaclyn Camuglia0https://orcid.org/0000-0001-9268-9471Soline Chanet1https://orcid.org/0000-0002-4065-9411Adam C Martin2https://orcid.org/0000-0001-8060-2607Biology Department, Massachusetts Institute of Technology, Cambridge, MA, United StatesCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, FranceBiology Department, Massachusetts Institute of Technology, Cambridge, MA, United StatesSpindle orientation is often achieved by a complex of Partner of Inscuteable (Pins)/LGN, Mushroom Body Defect (Mud)/Nuclear Mitotic Apparatus (NuMa), Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this process. Here, we identify Pins-mediated planar cell polarized divisions in several of the mitotic domains of the early Drosophila embryo. We found that neither planar cell polarity pathways nor planar polarized myosin localization determined division orientation; instead, our findings strongly suggest that Pins planar polarity and force generated from mesoderm invagination are important. Disrupting Pins polarity via overexpression of a myristoylated version of Pins caused randomized division angles. We found that disrupting forces through chemical inhibitors, depletion of an adherens junction protein, or blocking mesoderm invagination disrupted Pins planar polarity and spindle orientation. Furthermore, directional ablations that separated mesoderm from mitotic domains disrupted spindle orientation, suggesting that forces transmitted from mesoderm to mitotic domains can polarize Pins and orient division during gastrulation. To our knowledge, this is the first in vivo example where mechanical force has been shown to polarize Pins to mediate division orientation.https://elifesciences.org/articles/78779spindle orientationmechanicsmorphogenesisadherens junctionsPins/LGNDrosophila |
| spellingShingle | Jaclyn Camuglia Soline Chanet Adam C Martin Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation eLife spindle orientation mechanics morphogenesis adherens junctions Pins/LGN Drosophila |
| title | Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation |
| title_full | Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation |
| title_fullStr | Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation |
| title_full_unstemmed | Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation |
| title_short | Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation |
| title_sort | morphogenetic forces planar polarize lgn pins in the embryonic head during drosophila gastrulation |
| topic | spindle orientation mechanics morphogenesis adherens junctions Pins/LGN Drosophila |
| url | https://elifesciences.org/articles/78779 |
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