Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety
Anxiety disorders are the most frequently diagnosed psychological condition, associated with serious comorbidities including excessive fear and interference with daily life. Drugs for anxiety disorders are typically prescribed but the side effects include weight gain, nausea, and sleepiness. Exercis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2021.665800/full |
| _version_ | 1818887704744558592 |
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| author | Kyeong Jin Yoon Suhong Park Seung Hee Kwak Hyo Youl Moon Hyo Youl Moon Hyo Youl Moon |
| author_facet | Kyeong Jin Yoon Suhong Park Seung Hee Kwak Hyo Youl Moon Hyo Youl Moon Hyo Youl Moon |
| author_sort | Kyeong Jin Yoon |
| collection | DOAJ |
| description | Anxiety disorders are the most frequently diagnosed psychological condition, associated with serious comorbidities including excessive fear and interference with daily life. Drugs for anxiety disorders are typically prescribed but the side effects include weight gain, nausea, and sleepiness. Exercise is an effective treatment for anxiety. Exercise induces the release of extracellular vesicles (EVs) into the circulation, which transmit signals between organs. However, the effects of exercise-induced EVs on anxiety remain poorly understood. Here, we isolated EVs from the sera of mice that were sedentary or that voluntarily exercised. We characterized the changes in the miRNA profile of serum EVs after 4 weeks of voluntary exercise. miRNA sequencing showed that 82 miRNAs (46 of which were positive and 36 negative regulators) changed after exercise. We selected genes affected by at least two miRNAs. Of these, 27.27% were associated with neurotrophin signaling (9.09% with each of central nervous system neuronal development, cerebral cortical cell migration, and peripheral neuronal development). We also analyzed behavioral changes in mice with 3 weeks of restraint stress-induced anxiety after injection of 20 μg amounts of EVs from exercised or sedentary mice into the left cerebral ventricle. We found that exercise-derived EVs reduced anxiety (compared to a control group) in a nest-building test but found no between-group differences in the rotarod or open field tests. Exercise-derived EVs enhanced the expression of neuroactive ligand-receptor interaction genes. Thus, exercise-derived EVs may exhibit anti-anxiety effects and may be of therapeutic utility. |
| first_indexed | 2024-12-19T16:41:28Z |
| format | Article |
| id | doaj.art-6b62fb480d734d0a8694bbc91652b7df |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-19T16:41:28Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-6b62fb480d734d0a8694bbc91652b7df2022-12-21T20:13:46ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992021-07-011410.3389/fnmol.2021.665800665800Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on AnxietyKyeong Jin Yoon0Suhong Park1Seung Hee Kwak2Hyo Youl Moon3Hyo Youl Moon4Hyo Youl Moon5Department of Physical Education, Seoul National University, Seoul, South KoreaDepartment of Physical Education, Seoul National University, Seoul, South KoreaDepartment of Physical Education, Seoul National University, Seoul, South KoreaDepartment of Physical Education, Seoul National University, Seoul, South KoreaInstitute of Sport Science, Seoul National University, Seoul, South KoreaInstitute on Aging, Seoul National University, Seoul, South KoreaAnxiety disorders are the most frequently diagnosed psychological condition, associated with serious comorbidities including excessive fear and interference with daily life. Drugs for anxiety disorders are typically prescribed but the side effects include weight gain, nausea, and sleepiness. Exercise is an effective treatment for anxiety. Exercise induces the release of extracellular vesicles (EVs) into the circulation, which transmit signals between organs. However, the effects of exercise-induced EVs on anxiety remain poorly understood. Here, we isolated EVs from the sera of mice that were sedentary or that voluntarily exercised. We characterized the changes in the miRNA profile of serum EVs after 4 weeks of voluntary exercise. miRNA sequencing showed that 82 miRNAs (46 of which were positive and 36 negative regulators) changed after exercise. We selected genes affected by at least two miRNAs. Of these, 27.27% were associated with neurotrophin signaling (9.09% with each of central nervous system neuronal development, cerebral cortical cell migration, and peripheral neuronal development). We also analyzed behavioral changes in mice with 3 weeks of restraint stress-induced anxiety after injection of 20 μg amounts of EVs from exercised or sedentary mice into the left cerebral ventricle. We found that exercise-derived EVs reduced anxiety (compared to a control group) in a nest-building test but found no between-group differences in the rotarod or open field tests. Exercise-derived EVs enhanced the expression of neuroactive ligand-receptor interaction genes. Thus, exercise-derived EVs may exhibit anti-anxiety effects and may be of therapeutic utility.https://www.frontiersin.org/articles/10.3389/fnmol.2021.665800/fullexerciseextracellular vesicleanxietymiRNAICV injection |
| spellingShingle | Kyeong Jin Yoon Suhong Park Seung Hee Kwak Hyo Youl Moon Hyo Youl Moon Hyo Youl Moon Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety Frontiers in Molecular Neuroscience exercise extracellular vesicle anxiety miRNA ICV injection |
| title | Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety |
| title_full | Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety |
| title_fullStr | Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety |
| title_full_unstemmed | Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety |
| title_short | Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety |
| title_sort | effects of voluntary running wheel exercise induced extracellular vesicles on anxiety |
| topic | exercise extracellular vesicle anxiety miRNA ICV injection |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2021.665800/full |
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