Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells
This study aims to determine the difference in the inhibitory effect of temozolomide (TMZ) on TJ905 glioma cells and stem cells. TJ905 cancer stem cells were isolated. Livin is a member of the inhibitor of apoptosis protein family. The TJ905 cells and cancer stem cells were transfected with a Livin-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Neurology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fneur.2017.00474/full |
| _version_ | 1819083643500363776 |
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| author | Feng Jin Guang-Kui Han Hao Zhang Ran Zhang Gen-Hua Li Song Feng Xian-Yun Qin Ling-Sheng Kong Quan-Min Nie Hua-Rong Li Lei Zhao |
| author_facet | Feng Jin Guang-Kui Han Hao Zhang Ran Zhang Gen-Hua Li Song Feng Xian-Yun Qin Ling-Sheng Kong Quan-Min Nie Hua-Rong Li Lei Zhao |
| author_sort | Feng Jin |
| collection | DOAJ |
| description | This study aims to determine the difference in the inhibitory effect of temozolomide (TMZ) on TJ905 glioma cells and stem cells. TJ905 cancer stem cells were isolated. Livin is a member of the inhibitor of apoptosis protein family. The TJ905 cells and cancer stem cells were transfected with a Livin-shRNA and negative-shRNA, respectively, and then treated with TMZ. At 48 h post-transfection, a cell counting kit 8 assay, flow cytometry, and real-time qPCR were performed to detect cell proliferation, the cell cycle, and the expression of the Caspase-3, -7, and -9 mRNAs, respectively. As a result, the suppressive effect of TMZ on TJ905 cells was more significant than its effect on TJ905 cancer stem cells. TMZ exerted an inhibitory effect on the growth of TJ905 glioma cells by arresting them at G0/G1 phase and arresting cancer stem cells at S phase in a dose-dependent manner. TMZ inhibited Livin mRNA expression and increased the expression of the Caspase-3, -7, and -9 mRNAs. Low Livin mRNA expression induced high levels of Caspase-3, -7, and -9 expressions, thus promoting the apoptosis of both TJ905 cells and cancer stem cells in response to TMZ treatment. The TJ905 cells transfected with the Livin-shRNA were more sensitive to TMZ, whereas the TJ905 glioma stem cells transfected with the Livin-shRNA showed no significant changes in their sensitivity to TMZ. In conclusion, the Livin gene may play an important role in the resistance mechanisms of TJ905 glioma cells and cancer stem cells. However, Livin had a more distinct role in TMZ resistance, cell proliferation, and the cell cycle in TJ905 glioma cells than in cancer stem cells. |
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| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-21T20:35:50Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-6b64cfac05204f8da6312e3feabbd5e82022-12-21T18:51:07ZengFrontiers Media S.A.Frontiers in Neurology1664-22952017-09-01810.3389/fneur.2017.00474262209Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem CellsFeng Jin0Guang-Kui Han1Hao Zhang2Ran Zhang3Gen-Hua Li4Song Feng5Xian-Yun Qin6Ling-Sheng Kong7Quan-Min Nie8Hua-Rong Li9Lei Zhao10Department of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Shandong Provincial Key Laboratory of Stem Cells and Neuro-Oncology, Jining, ChinaDepartment of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaThis study aims to determine the difference in the inhibitory effect of temozolomide (TMZ) on TJ905 glioma cells and stem cells. TJ905 cancer stem cells were isolated. Livin is a member of the inhibitor of apoptosis protein family. The TJ905 cells and cancer stem cells were transfected with a Livin-shRNA and negative-shRNA, respectively, and then treated with TMZ. At 48 h post-transfection, a cell counting kit 8 assay, flow cytometry, and real-time qPCR were performed to detect cell proliferation, the cell cycle, and the expression of the Caspase-3, -7, and -9 mRNAs, respectively. As a result, the suppressive effect of TMZ on TJ905 cells was more significant than its effect on TJ905 cancer stem cells. TMZ exerted an inhibitory effect on the growth of TJ905 glioma cells by arresting them at G0/G1 phase and arresting cancer stem cells at S phase in a dose-dependent manner. TMZ inhibited Livin mRNA expression and increased the expression of the Caspase-3, -7, and -9 mRNAs. Low Livin mRNA expression induced high levels of Caspase-3, -7, and -9 expressions, thus promoting the apoptosis of both TJ905 cells and cancer stem cells in response to TMZ treatment. The TJ905 cells transfected with the Livin-shRNA were more sensitive to TMZ, whereas the TJ905 glioma stem cells transfected with the Livin-shRNA showed no significant changes in their sensitivity to TMZ. In conclusion, the Livin gene may play an important role in the resistance mechanisms of TJ905 glioma cells and cancer stem cells. However, Livin had a more distinct role in TMZ resistance, cell proliferation, and the cell cycle in TJ905 glioma cells than in cancer stem cells.http://journal.frontiersin.org/article/10.3389/fneur.2017.00474/fullLivintemozolomidegliomacancer stem cellsproliferation |
| spellingShingle | Feng Jin Guang-Kui Han Hao Zhang Ran Zhang Gen-Hua Li Song Feng Xian-Yun Qin Ling-Sheng Kong Quan-Min Nie Hua-Rong Li Lei Zhao Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells Frontiers in Neurology Livin temozolomide glioma cancer stem cells proliferation |
| title | Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells |
| title_full | Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells |
| title_fullStr | Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells |
| title_full_unstemmed | Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells |
| title_short | Difference in the Inhibitory Effect of Temozolomide on TJ905 Glioma Cells and Stem Cells |
| title_sort | difference in the inhibitory effect of temozolomide on tj905 glioma cells and stem cells |
| topic | Livin temozolomide glioma cancer stem cells proliferation |
| url | http://journal.frontiersin.org/article/10.3389/fneur.2017.00474/full |
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