The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis
Jinyun Li,1,2,* Chongchang Zhou,1,* Haojie Zhou,3,* Tianlian Bao,1 Tengjiao Gao,1 Xiangling Jiang,1 Meng Ye1,2 1Department of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, 2Department of Medical Oncology, Affiliated Hospital, Ningbo University, 3Department of Molecular D...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2016-08-01
|
| Series: | Therapeutics and Clinical Risk Management |
| Subjects: | |
| Online Access: | https://www.dovepress.com/the-association-between-methylated-cdkn2a--and-cervical-carcinogenesis-peer-reviewed-article-TCRM |
| _version_ | 1819034858651910144 |
|---|---|
| author | Li J Zhou C Zhou H Bao T Gao T Jiang X Ye M |
| author_facet | Li J Zhou C Zhou H Bao T Gao T Jiang X Ye M |
| author_sort | Li J |
| collection | DOAJ |
| description | Jinyun Li,1,2,* Chongchang Zhou,1,* Haojie Zhou,3,* Tianlian Bao,1 Tengjiao Gao,1 Xiangling Jiang,1 Meng Ye1,2 1Department of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, 2Department of Medical Oncology, Affiliated Hospital, Ningbo University, 3Department of Molecular Diagnosis, Ningbo Diagnostic Pathology Center, Ningbo, Zhejiang, People’s Republic of China *These authors are co-first authors of this work Background: Cervical cancer is the second deadliest gynecologic malignancy, characterized by apparently precancerous lesions and cervical intraepithelial neoplasia (CIN), and having a long course from the development of CIN to cervical cancer. Cyclin-dependent kinase inhibitor 2A (CDKN2A) is a well-documented tumor suppressor gene and is commonly methylated in cervical cancer. However, the relationship between methylated CDKN2A and carcinogenesis in cervical cancer is inconsistent, and the diagnostic accuracy of methylated CDKN2A is underinvestigated. In this study, we attempted to quantify the association between CDKN2A methylation and the carcinogenesis of cervical cancer, and its diagnostic power.Methods: We systematically reviewed four electronic databases and identified 26 studies involving 1,490 cervical cancers, 1,291 CINs, and 964 controls. A pooled odds ratio (OR) with corresponding 95% confidence intervals (95% CI) was calculated to evaluate the association between methylated CDKN2A and the carcinogenesis of cervical cancer. Specificity, sensitivity, the area under the receiver operating characteristic curve, and the diagnostic odds ratio were computed to assess the effect of methylated CDKN2A in the diagnosis of cervical cancer.Results: Our results indicated an upward trend in the methylation frequency of CDKN2A in the carcinogenesis of cervical cancer (cancer vs control: OR =23.67, 95% CI =15.54–36.06; cancer vs CIN: OR =2.53, 95% CI =1.79–3.5; CIN vs control: OR =9.68, 95% CI =5.82–16.02). The specificity, sensitivity, area under the receiver operating characteristic curve, and diagnostic odds ratio were 0.99 (95% CI: 0.97–0.99), 0.36 (95% CI: 0.28–0.45), 0.93 (95% CI: 0.91–0.95), and 43 (95% CI: 19–98), respectively.Conclusion: Our findings indicate that abnormal CDKN2A methylation may be strongly correlated with the pathogenesis of cervical cancer. Our results also demonstrate that CDKN2A methylation might serve as an early detector of cervical cancer. These findings require further confirmation. Keywords: p16, methylation, cervical cancer carcinogenesis |
| first_indexed | 2024-12-21T07:40:25Z |
| format | Article |
| id | doaj.art-6b699e62770a4617868f91bc6ff40a33 |
| institution | Directory Open Access Journal |
| issn | 1178-203X |
| language | English |
| last_indexed | 2024-12-21T07:40:25Z |
| publishDate | 2016-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Therapeutics and Clinical Risk Management |
| spelling | doaj.art-6b699e62770a4617868f91bc6ff40a332022-12-21T19:11:20ZengDove Medical PressTherapeutics and Clinical Risk Management1178-203X2016-08-01Volume 121249126028480The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysisLi JZhou CZhou HBao TGao TJiang XYe MJinyun Li,1,2,* Chongchang Zhou,1,* Haojie Zhou,3,* Tianlian Bao,1 Tengjiao Gao,1 Xiangling Jiang,1 Meng Ye1,2 1Department of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, 2Department of Medical Oncology, Affiliated Hospital, Ningbo University, 3Department of Molecular Diagnosis, Ningbo Diagnostic Pathology Center, Ningbo, Zhejiang, People’s Republic of China *These authors are co-first authors of this work Background: Cervical cancer is the second deadliest gynecologic malignancy, characterized by apparently precancerous lesions and cervical intraepithelial neoplasia (CIN), and having a long course from the development of CIN to cervical cancer. Cyclin-dependent kinase inhibitor 2A (CDKN2A) is a well-documented tumor suppressor gene and is commonly methylated in cervical cancer. However, the relationship between methylated CDKN2A and carcinogenesis in cervical cancer is inconsistent, and the diagnostic accuracy of methylated CDKN2A is underinvestigated. In this study, we attempted to quantify the association between CDKN2A methylation and the carcinogenesis of cervical cancer, and its diagnostic power.Methods: We systematically reviewed four electronic databases and identified 26 studies involving 1,490 cervical cancers, 1,291 CINs, and 964 controls. A pooled odds ratio (OR) with corresponding 95% confidence intervals (95% CI) was calculated to evaluate the association between methylated CDKN2A and the carcinogenesis of cervical cancer. Specificity, sensitivity, the area under the receiver operating characteristic curve, and the diagnostic odds ratio were computed to assess the effect of methylated CDKN2A in the diagnosis of cervical cancer.Results: Our results indicated an upward trend in the methylation frequency of CDKN2A in the carcinogenesis of cervical cancer (cancer vs control: OR =23.67, 95% CI =15.54–36.06; cancer vs CIN: OR =2.53, 95% CI =1.79–3.5; CIN vs control: OR =9.68, 95% CI =5.82–16.02). The specificity, sensitivity, area under the receiver operating characteristic curve, and diagnostic odds ratio were 0.99 (95% CI: 0.97–0.99), 0.36 (95% CI: 0.28–0.45), 0.93 (95% CI: 0.91–0.95), and 43 (95% CI: 19–98), respectively.Conclusion: Our findings indicate that abnormal CDKN2A methylation may be strongly correlated with the pathogenesis of cervical cancer. Our results also demonstrate that CDKN2A methylation might serve as an early detector of cervical cancer. These findings require further confirmation. Keywords: p16, methylation, cervical cancer carcinogenesishttps://www.dovepress.com/the-association-between-methylated-cdkn2a--and-cervical-carcinogenesis-peer-reviewed-article-TCRMp16methylationcervical cancer carcinogenesis |
| spellingShingle | Li J Zhou C Zhou H Bao T Gao T Jiang X Ye M The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis Therapeutics and Clinical Risk Management p16 methylation cervical cancer carcinogenesis |
| title | The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis |
| title_full | The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis |
| title_fullStr | The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis |
| title_full_unstemmed | The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis |
| title_short | The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis |
| title_sort | association between methylated cdkn2a and cervical carcinogenesis and its diagnostic value in cervical cancer a meta analysis |
| topic | p16 methylation cervical cancer carcinogenesis |
| url | https://www.dovepress.com/the-association-between-methylated-cdkn2a--and-cervical-carcinogenesis-peer-reviewed-article-TCRM |
| work_keys_str_mv | AT lij theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT zhouc theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT zhouh theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT baot theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT gaot theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT jiangx theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT yem theassociationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT lij associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT zhouc associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT zhouh associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT baot associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT gaot associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT jiangx associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis AT yem associationbetweenmethylatedcdkn2aandcervicalcarcinogenesisanditsdiagnosticvalueincervicalcancerametaanalysis |