Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats
Supplementation with natural bioactive compounds has been proposed to be a complementary tool to the calorie-restricted diets and physical exercise programs used to tackle human overweight, obesity and Metabolic syndrome. Herein, we evaluated the effects of 14 weeks of calorie-restricted cafeteria d...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/147 |
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| author | Alex Subias-Gusils Adam Álvarez-Monell Noemi Boqué Antoni Caimari Roger Mariné-Casadó Rosa M. Escorihuela Montserrat Solanas |
| author_facet | Alex Subias-Gusils Adam Álvarez-Monell Noemi Boqué Antoni Caimari Roger Mariné-Casadó Rosa M. Escorihuela Montserrat Solanas |
| author_sort | Alex Subias-Gusils |
| collection | DOAJ |
| description | Supplementation with natural bioactive compounds has been proposed to be a complementary tool to the calorie-restricted diets and physical exercise programs used to tackle human overweight, obesity and Metabolic syndrome. Herein, we evaluated the effects of 14 weeks of calorie-restricted cafeteria diet either alone or combined with oral administration of the polyphenol oleuropein in obese adult male rats, compared with a control group fed standard chow and a group fed cafeteria diet. Animals were sacrificed at the age of 26 weeks and several tissues of interest were removed. The results showed that both dietary interventions reduced the adiposity index (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively), and specifically the abdominal fat depots (mesenteric: <i>p</i> < 0.01 and <i>p</i> < 0.01, respectively; and epididymal: both diets <i>p</i> < 0.001) and restored the decreased soleus skeletal muscle mass. Both interventions decreased leptin mRNA expression in mesenteric white adipose tissue (<i>p</i> < 0.05) and normalized hypothalamic <i>Agrp</i> mRNA expression compared to cafeteria-fed obese rats (<i>p</i> < 0.05). However, only the calorie-restricted cafeteria diet supplemented with oleuropein induced additional lower retroperitoneal adipose accretion (<i>p</i> < 0.05) and increased hypothalamic leptin receptor mRNA levels (<i>p</i> < 0.05). Experiments with female animals, at different doses and longer intervention periods, are needed to better determine the potential benefits of this dietary treatment. |
| first_indexed | 2024-03-11T08:26:15Z |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:26:15Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-6b69e45cba204e538f6cba794f831abf2023-11-16T22:03:33ZengMDPI AGMetabolites2218-19892023-01-0113214710.3390/metabo13020147Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male RatsAlex Subias-Gusils0Adam Álvarez-Monell1Noemi Boqué2Antoni Caimari3Roger Mariné-Casadó4Rosa M. Escorihuela5Montserrat Solanas6Institut de Neurociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainInstitut de Neurociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainEurecat, Centre Tecnològic de Catalunya, Biotechnology Area and Technological Unit of Nutrition and Health, 43204 Reus, SpainEurecat, Centre Tecnològic de Catalunya, Biotechnology Area and Technological Unit of Nutrition and Health, 43204 Reus, SpainEurecat, Centre Tecnològic de Catalunya, Biotechnology Area and Technological Unit of Nutrition and Health, 43204 Reus, SpainInstitut de Neurociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainInstitut de Neurociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainSupplementation with natural bioactive compounds has been proposed to be a complementary tool to the calorie-restricted diets and physical exercise programs used to tackle human overweight, obesity and Metabolic syndrome. Herein, we evaluated the effects of 14 weeks of calorie-restricted cafeteria diet either alone or combined with oral administration of the polyphenol oleuropein in obese adult male rats, compared with a control group fed standard chow and a group fed cafeteria diet. Animals were sacrificed at the age of 26 weeks and several tissues of interest were removed. The results showed that both dietary interventions reduced the adiposity index (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively), and specifically the abdominal fat depots (mesenteric: <i>p</i> < 0.01 and <i>p</i> < 0.01, respectively; and epididymal: both diets <i>p</i> < 0.001) and restored the decreased soleus skeletal muscle mass. Both interventions decreased leptin mRNA expression in mesenteric white adipose tissue (<i>p</i> < 0.05) and normalized hypothalamic <i>Agrp</i> mRNA expression compared to cafeteria-fed obese rats (<i>p</i> < 0.05). However, only the calorie-restricted cafeteria diet supplemented with oleuropein induced additional lower retroperitoneal adipose accretion (<i>p</i> < 0.05) and increased hypothalamic leptin receptor mRNA levels (<i>p</i> < 0.05). Experiments with female animals, at different doses and longer intervention periods, are needed to better determine the potential benefits of this dietary treatment.https://www.mdpi.com/2218-1989/13/2/147adiponectincalorie restrictiondietary supplementshypothalamusleptinmetabolic syndrome |
| spellingShingle | Alex Subias-Gusils Adam Álvarez-Monell Noemi Boqué Antoni Caimari Roger Mariné-Casadó Rosa M. Escorihuela Montserrat Solanas Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats Metabolites adiponectin calorie restriction dietary supplements hypothalamus leptin metabolic syndrome |
| title | Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats |
| title_full | Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats |
| title_fullStr | Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats |
| title_full_unstemmed | Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats |
| title_short | Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats |
| title_sort | effects of a calorie restricted cafeteria diet and oleuropein supplementation on adiposity and mrna expression of energy balance related genes in obese male rats |
| topic | adiponectin calorie restriction dietary supplements hypothalamus leptin metabolic syndrome |
| url | https://www.mdpi.com/2218-1989/13/2/147 |
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