CTmax is repeatable and doesn’t reduce growth in zebrafish
Abstract Critical thermal maximum (CTmax) is a commonly and increasingly used measure of an animal’s upper thermal tolerance limit. However, it is unknown how consistent CTmax is within an individual, and how physiologically taxing such experiments are. We addressed this by estimating the repeatabil...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-25593-4 |
| _version_ | 1818431066666434560 |
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| author | Rachael Morgan Mette H. Finnøen Fredrik Jutfelt |
| author_facet | Rachael Morgan Mette H. Finnøen Fredrik Jutfelt |
| author_sort | Rachael Morgan |
| collection | DOAJ |
| description | Abstract Critical thermal maximum (CTmax) is a commonly and increasingly used measure of an animal’s upper thermal tolerance limit. However, it is unknown how consistent CTmax is within an individual, and how physiologically taxing such experiments are. We addressed this by estimating the repeatability of CTmax in zebrafish, and measured how growth and survival were affected by multiple trials. The repeatability of CTmax over four trials was 0.22 (0.07–0.43). However, CTmax increased from the first to the second trial, likely because of thermal acclimation triggered by the heat shock. After this initial acclimation response individuals became more consistent in their CTmax, reflected in a higher repeatability measure of 0.45 (0.28–0.65) for trials 2–4. We found a high innate thermal tolerance led to a lower acclimation response, whereas a high acclimation response was present in individuals that displayed a low initial CTmax. This could indicate that different strategies for thermal tolerance (i.e. plasticity vs. high innate tolerance) can co-exist in a population. Additionally, repeated CTmax trials had no effect on growth, and survival was high (99%). This validates the method and, combined with the relatively high repeatability, highlights the relevance of CTmax for continued use as a metric for acute thermal tolerance. |
| first_indexed | 2024-12-14T15:43:24Z |
| format | Article |
| id | doaj.art-6b6a4c4b77a64ec097a25fdb81be60b9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:43:24Z |
| publishDate | 2018-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6b6a4c4b77a64ec097a25fdb81be60b92022-12-21T22:55:34ZengNature PortfolioScientific Reports2045-23222018-05-01811810.1038/s41598-018-25593-4CTmax is repeatable and doesn’t reduce growth in zebrafishRachael Morgan0Mette H. Finnøen1Fredrik Jutfelt2Department of Biology, Norwegian University of Science and TechnologyDepartment of Biology, Norwegian University of Science and TechnologyDepartment of Biology, Norwegian University of Science and TechnologyAbstract Critical thermal maximum (CTmax) is a commonly and increasingly used measure of an animal’s upper thermal tolerance limit. However, it is unknown how consistent CTmax is within an individual, and how physiologically taxing such experiments are. We addressed this by estimating the repeatability of CTmax in zebrafish, and measured how growth and survival were affected by multiple trials. The repeatability of CTmax over four trials was 0.22 (0.07–0.43). However, CTmax increased from the first to the second trial, likely because of thermal acclimation triggered by the heat shock. After this initial acclimation response individuals became more consistent in their CTmax, reflected in a higher repeatability measure of 0.45 (0.28–0.65) for trials 2–4. We found a high innate thermal tolerance led to a lower acclimation response, whereas a high acclimation response was present in individuals that displayed a low initial CTmax. This could indicate that different strategies for thermal tolerance (i.e. plasticity vs. high innate tolerance) can co-exist in a population. Additionally, repeated CTmax trials had no effect on growth, and survival was high (99%). This validates the method and, combined with the relatively high repeatability, highlights the relevance of CTmax for continued use as a metric for acute thermal tolerance.https://doi.org/10.1038/s41598-018-25593-4 |
| spellingShingle | Rachael Morgan Mette H. Finnøen Fredrik Jutfelt CTmax is repeatable and doesn’t reduce growth in zebrafish Scientific Reports |
| title | CTmax is repeatable and doesn’t reduce growth in zebrafish |
| title_full | CTmax is repeatable and doesn’t reduce growth in zebrafish |
| title_fullStr | CTmax is repeatable and doesn’t reduce growth in zebrafish |
| title_full_unstemmed | CTmax is repeatable and doesn’t reduce growth in zebrafish |
| title_short | CTmax is repeatable and doesn’t reduce growth in zebrafish |
| title_sort | ctmax is repeatable and doesn t reduce growth in zebrafish |
| url | https://doi.org/10.1038/s41598-018-25593-4 |
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