Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device
Abstract The central nervous system is organized into different neural circuits, each with particular functions and properties. Studying neural circuits is essential to understanding brain function and neuronal diseases. Microfluidic systems are widely used for reconstructing and studying neural cir...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-01-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13041-023-01007-z |
| _version_ | 1797945675352113152 |
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| author | Sukmin Han Seokyoung Bang Hong Nam Kim Nakwon Choi Sung Hyun Kim |
| author_facet | Sukmin Han Seokyoung Bang Hong Nam Kim Nakwon Choi Sung Hyun Kim |
| author_sort | Sukmin Han |
| collection | DOAJ |
| description | Abstract The central nervous system is organized into different neural circuits, each with particular functions and properties. Studying neural circuits is essential to understanding brain function and neuronal diseases. Microfluidic systems are widely used for reconstructing and studying neural circuits but still need improvement to allow modulation and monitoring of the physiological properties of circuits. In this study, we constructed an improved microfluidic device that supports the electrical modulation of neural circuits and proper reassembly. We demonstrated that our microfluidic device provides a platform for electrically modulating and monitoring the physiological function of neural circuits with genetic indicators for synaptic functionality in corticostriatal (CStr) circuits. In particular, our microfluidic device measures activity-driven Ca2+ dynamics using Ca2+ indicators (synaptophysin-GCaMP6f and Fluo5F-AM), as well as activity-driven synaptic transmission and retrieval using vGlut-pHluorin. Overall, our findings indicate that the improved microfluidic platform described here is an invaluable tool for studying the physiological properties of specific neural circuits. |
| first_indexed | 2024-04-10T20:58:51Z |
| format | Article |
| id | doaj.art-6b6c67cfc6bb4922ab004d407bfbddd8 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-04-10T20:58:51Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-6b6c67cfc6bb4922ab004d407bfbddd82023-01-22T12:28:44ZengBMCMolecular Brain1756-66062023-01-0116111010.1186/s13041-023-01007-zModulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic deviceSukmin Han0Seokyoung Bang1Hong Nam Kim2Nakwon Choi3Sung Hyun Kim4Department of Neuroscience, Graduate School, Kyung Hee UniversityBrain Science Institute, Korea Institute of Science and Technology (KIST)Brain Science Institute, Korea Institute of Science and Technology (KIST)Brain Science Institute, Korea Institute of Science and Technology (KIST)Department of Neuroscience, Graduate School, Kyung Hee UniversityAbstract The central nervous system is organized into different neural circuits, each with particular functions and properties. Studying neural circuits is essential to understanding brain function and neuronal diseases. Microfluidic systems are widely used for reconstructing and studying neural circuits but still need improvement to allow modulation and monitoring of the physiological properties of circuits. In this study, we constructed an improved microfluidic device that supports the electrical modulation of neural circuits and proper reassembly. We demonstrated that our microfluidic device provides a platform for electrically modulating and monitoring the physiological function of neural circuits with genetic indicators for synaptic functionality in corticostriatal (CStr) circuits. In particular, our microfluidic device measures activity-driven Ca2+ dynamics using Ca2+ indicators (synaptophysin-GCaMP6f and Fluo5F-AM), as well as activity-driven synaptic transmission and retrieval using vGlut-pHluorin. Overall, our findings indicate that the improved microfluidic platform described here is an invaluable tool for studying the physiological properties of specific neural circuits.https://doi.org/10.1186/s13041-023-01007-zMicrofluidic deviceCorticostriatal (CStr) circuitSynapseCa2+ dynamicsAction potentialSynaptic transmission |
| spellingShingle | Sukmin Han Seokyoung Bang Hong Nam Kim Nakwon Choi Sung Hyun Kim Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device Molecular Brain Microfluidic device Corticostriatal (CStr) circuit Synapse Ca2+ dynamics Action potential Synaptic transmission |
| title | Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device |
| title_full | Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device |
| title_fullStr | Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device |
| title_full_unstemmed | Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device |
| title_short | Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device |
| title_sort | modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device |
| topic | Microfluidic device Corticostriatal (CStr) circuit Synapse Ca2+ dynamics Action potential Synaptic transmission |
| url | https://doi.org/10.1186/s13041-023-01007-z |
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