Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143
The study objective is to investigate the features of subpopulational composition of mobilized hematopoietic stem cells in peripheral blood (PB) and leukocyte concentrates (LC) in adult patients with oncohematological pathology and donors.Materials and methods. In 80 patients with hemoblastoses, exp...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2019-07-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/348 |
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| author | M. L. Kanaeva I. V. Galtseva E. N. Parovichnikova Yu. O. Davydova T. V. Gaponova E. O. Gribanova Ya. V. Balzhanova L. A. Kuzmina V. V. Troitskaya S. K. Kravchenko E. E. Zvonkov L. P. Mendeleeva V. G. Savchenko |
| author_facet | M. L. Kanaeva I. V. Galtseva E. N. Parovichnikova Yu. O. Davydova T. V. Gaponova E. O. Gribanova Ya. V. Balzhanova L. A. Kuzmina V. V. Troitskaya S. K. Kravchenko E. E. Zvonkov L. P. Mendeleeva V. G. Savchenko |
| author_sort | M. L. Kanaeva |
| collection | DOAJ |
| description | The study objective is to investigate the features of subpopulational composition of mobilized hematopoietic stem cells in peripheral blood (PB) and leukocyte concentrates (LC) in adult patients with oncohematological pathology and donors.Materials and methods. In 80 patients with hemoblastoses, expression of CD38, HLA-DR and CD143 (angiotensin-converting enzyme) was measured in PB and LC CD34+CD45low cells. The control group included 10 PB and 14 LC samples from healthy donors. Analysis of PB was performed prior to mobilization of hematopoietic stem cells (HSC) and on the day of leukapheresis prior to HSC collection. LC samples were examined at day 1 after HSC collection.Results. CD143 is expressed on CD34+CD45low cells both prior to mobilization and after it in all patients and donors, but CD34+CD45lowCD143+ cell counts varied depending on diagnosis and mobilization regimen. CD143+ expression on CD34+CD45low cells was significantly higher in patients who received combination of chemotherapy and granulocyte colony-stimulating factor compared to donors and patients with multiple myeloma who received only granulocyte colony-stimulating factor. Along with elevated CD34+CD45low cell count after hematopoiesis stimulation, CD34+CD45lowCD143+ cell counts also increased. It was shown that mobilized HSC almost completely lacks a fraction of early CD34+CD45low progenitor cells not expressing CD38, HLA-DR. Prior to hematopoiesis stimulation among CD34+CD45low cells, CD38+HLADR–cell fractions are prevalent, but after mobilization CD38–HLA-DR+ cell counts increased. No differences between CD34+CD45lowCD143+cell counts in patients with multiple myeloma depending on disease status, sex, age or number of chemotherapy courses prior to HSC mobilizationwere observed.Conclusion. Expression of angiotensin-converting enzyme on CD34+ cells in PB before and after HSC mobilization and in LC was observed. The cell counts varied depending on diagnosis and mobilization regimen. |
| first_indexed | 2024-04-09T20:25:27Z |
| format | Article |
| id | doaj.art-6b6c684cd9604a70ab91bbb4fe2272ca |
| institution | Directory Open Access Journal |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| last_indexed | 2025-03-14T08:24:02Z |
| publishDate | 2019-07-01 |
| publisher | ABV-press |
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| series | Онкогематология |
| spelling | doaj.art-6b6c684cd9604a70ab91bbb4fe2272ca2025-03-02T13:07:12ZrusABV-pressОнкогематология1818-83462413-40232019-07-01142485810.17650/1818-8346-2019-14-2-48-58313Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143M. L. Kanaeva0I. V. Galtseva1E. N. Parovichnikova2Yu. O. Davydova3T. V. Gaponova4E. O. Gribanova5Ya. V. Balzhanova6L. A. Kuzmina7V. V. Troitskaya8S. K. Kravchenko9E. E. Zvonkov10L. P. Mendeleeva11V. G. Savchenko12National Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaThe study objective is to investigate the features of subpopulational composition of mobilized hematopoietic stem cells in peripheral blood (PB) and leukocyte concentrates (LC) in adult patients with oncohematological pathology and donors.Materials and methods. In 80 patients with hemoblastoses, expression of CD38, HLA-DR and CD143 (angiotensin-converting enzyme) was measured in PB and LC CD34+CD45low cells. The control group included 10 PB and 14 LC samples from healthy donors. Analysis of PB was performed prior to mobilization of hematopoietic stem cells (HSC) and on the day of leukapheresis prior to HSC collection. LC samples were examined at day 1 after HSC collection.Results. CD143 is expressed on CD34+CD45low cells both prior to mobilization and after it in all patients and donors, but CD34+CD45lowCD143+ cell counts varied depending on diagnosis and mobilization regimen. CD143+ expression on CD34+CD45low cells was significantly higher in patients who received combination of chemotherapy and granulocyte colony-stimulating factor compared to donors and patients with multiple myeloma who received only granulocyte colony-stimulating factor. Along with elevated CD34+CD45low cell count after hematopoiesis stimulation, CD34+CD45lowCD143+ cell counts also increased. It was shown that mobilized HSC almost completely lacks a fraction of early CD34+CD45low progenitor cells not expressing CD38, HLA-DR. Prior to hematopoiesis stimulation among CD34+CD45low cells, CD38+HLADR–cell fractions are prevalent, but after mobilization CD38–HLA-DR+ cell counts increased. No differences between CD34+CD45lowCD143+cell counts in patients with multiple myeloma depending on disease status, sex, age or number of chemotherapy courses prior to HSC mobilizationwere observed.Conclusion. Expression of angiotensin-converting enzyme on CD34+ cells in PB before and after HSC mobilization and in LC was observed. The cell counts varied depending on diagnosis and mobilization regimen.https://oncohematology.abvpress.ru/ongm/article/view/348hematopoietic stem cellssubpopulationcd34cd38hla-dr expressionangiotensin-converting enzyme (cd143) |
| spellingShingle | M. L. Kanaeva I. V. Galtseva E. N. Parovichnikova Yu. O. Davydova T. V. Gaponova E. O. Gribanova Ya. V. Balzhanova L. A. Kuzmina V. V. Troitskaya S. K. Kravchenko E. E. Zvonkov L. P. Mendeleeva V. G. Savchenko Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143 Онкогематология hematopoietic stem cells subpopulation cd34 cd38 hla-dr expression angiotensin-converting enzyme (cd143) |
| title | Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143 |
| title_full | Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143 |
| title_fullStr | Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143 |
| title_full_unstemmed | Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143 |
| title_short | Subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors: expression of CD38, HLA-DR and CD143 |
| title_sort | subpopulations of mobilized hematopoietic stem cells in patients with hematological malignances and donors expression of cd38 hla dr and cd143 |
| topic | hematopoietic stem cells subpopulation cd34 cd38 hla-dr expression angiotensin-converting enzyme (cd143) |
| url | https://oncohematology.abvpress.ru/ongm/article/view/348 |
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