GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS
Aim. To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticytokine activity) in vitro. Materials and methods. The investigation of gut microsymbioce...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2016-12-01
|
| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/103 |
| _version_ | 1817983451944452096 |
|---|---|
| author | O. V. Bukharin O. E. Chelpachenko E. I. Danilova I. N. Chainikova N. B. Perunova E. V. Ivanova I. A. Nikiforov L. P. Fedotova T. A. Bondarenko A. V. Salgina |
| author_facet | O. V. Bukharin O. E. Chelpachenko E. I. Danilova I. N. Chainikova N. B. Perunova E. V. Ivanova I. A. Nikiforov L. P. Fedotova T. A. Bondarenko A. V. Salgina |
| author_sort | O. V. Bukharin |
| collection | DOAJ |
| description | Aim. To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticytokine activity) in vitro. Materials and methods. The investigation of gut microsymbiocenosis by means of bacteriological method was conducted in 34 children with RA and 25 relatively healthy 3 - 16 year- old children. Microorganisms were identified with the help of MALDI-TOF mass-spectrometry, anticytokine activity (АСА) of microsymbionts - according to Bukharin O.V. et al. (2011), biofilm formation - according to O’Toole G.A., Kolter R. (1998). Results. On the ground of species composition differences of gut microbiota discrimination model was created which allowed to separate the group of children with RA from healthy individuals. Microsymbiocenosis of patients with RAwas characterized by increasing number of opportunistic microorganisms (OM) (enterobacteria, clostridia, bacteroides, and Candida), BFF and АСА level. Conclusion. The obtained data greatly contribute to the deciphering of spondylo-arthritis and disclose the role of microbial factor under given pathology. Hypercolonisation of human gut with OM, having pronounced ability to BFF and regulating cytokine level, promotes strengthening of arthritogenic potential and serves as additional marker of arthritis development risk in children. |
| first_indexed | 2024-04-13T23:33:26Z |
| format | Article |
| id | doaj.art-6b6ca188f32346b6b12cf231f52757a1 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-13T23:33:26Z |
| publishDate | 2016-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-6b6ca188f32346b6b12cf231f52757a12022-12-22T02:24:49ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132016-12-0106414810.36233/0372-9311-2016-6-41-48103GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITISO. V. Bukharin0O. E. Chelpachenko1E. I. Danilova2I. N. Chainikova3N. B. Perunova4E. V. Ivanova5I. A. Nikiforov6L. P. Fedotova7T. A. Bondarenko8A. V. Salgina9Institute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisOrenburg State Medical UniversityInstitute of Cellular and Intracellular Symbiosis; Orenburg State Medical UniversityInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisAim. To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticytokine activity) in vitro. Materials and methods. The investigation of gut microsymbiocenosis by means of bacteriological method was conducted in 34 children with RA and 25 relatively healthy 3 - 16 year- old children. Microorganisms were identified with the help of MALDI-TOF mass-spectrometry, anticytokine activity (АСА) of microsymbionts - according to Bukharin O.V. et al. (2011), biofilm formation - according to O’Toole G.A., Kolter R. (1998). Results. On the ground of species composition differences of gut microbiota discrimination model was created which allowed to separate the group of children with RA from healthy individuals. Microsymbiocenosis of patients with RAwas characterized by increasing number of opportunistic microorganisms (OM) (enterobacteria, clostridia, bacteroides, and Candida), BFF and АСА level. Conclusion. The obtained data greatly contribute to the deciphering of spondylo-arthritis and disclose the role of microbial factor under given pathology. Hypercolonisation of human gut with OM, having pronounced ability to BFF and regulating cytokine level, promotes strengthening of arthritogenic potential and serves as additional marker of arthritis development risk in children.https://microbiol.elpub.ru/jour/article/view/103gut microsymbiocenosisanticytokine activitybiofilm formationreactive arthritischildren |
| spellingShingle | O. V. Bukharin O. E. Chelpachenko E. I. Danilova I. N. Chainikova N. B. Perunova E. V. Ivanova I. A. Nikiforov L. P. Fedotova T. A. Bondarenko A. V. Salgina GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS Журнал микробиологии, эпидемиологии и иммунобиологии gut microsymbiocenosis anticytokine activity biofilm formation reactive arthritis children |
| title | GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS |
| title_full | GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS |
| title_fullStr | GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS |
| title_full_unstemmed | GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS |
| title_short | GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS |
| title_sort | gut microsymbiocenosis in children with reactive arthritis |
| topic | gut microsymbiocenosis anticytokine activity biofilm formation reactive arthritis children |
| url | https://microbiol.elpub.ru/jour/article/view/103 |
| work_keys_str_mv | AT ovbukharin gutmicrosymbiocenosisinchildrenwithreactivearthritis AT oechelpachenko gutmicrosymbiocenosisinchildrenwithreactivearthritis AT eidanilova gutmicrosymbiocenosisinchildrenwithreactivearthritis AT inchainikova gutmicrosymbiocenosisinchildrenwithreactivearthritis AT nbperunova gutmicrosymbiocenosisinchildrenwithreactivearthritis AT evivanova gutmicrosymbiocenosisinchildrenwithreactivearthritis AT ianikiforov gutmicrosymbiocenosisinchildrenwithreactivearthritis AT lpfedotova gutmicrosymbiocenosisinchildrenwithreactivearthritis AT tabondarenko gutmicrosymbiocenosisinchildrenwithreactivearthritis AT avsalgina gutmicrosymbiocenosisinchildrenwithreactivearthritis |