A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range.
It is usually thought that bacteriophage T7 is female specific, while phage T3 can propagate on male and female Escherichia coli. We found that the growth patterns of phages T7M and T3 do not match the above characteristics, instead showing strain dependent male exclusion. Furthermore, a T3/7 hybrid...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3276506?pdf=render |
| _version_ | 1818296368178921472 |
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| author | Tiao-Yin Lin Yi-Haw Lo Pin-Wei Tseng Shun-Fu Chang Yann-Tsyr Lin Ton-Seng Chen |
| author_facet | Tiao-Yin Lin Yi-Haw Lo Pin-Wei Tseng Shun-Fu Chang Yann-Tsyr Lin Ton-Seng Chen |
| author_sort | Tiao-Yin Lin |
| collection | DOAJ |
| description | It is usually thought that bacteriophage T7 is female specific, while phage T3 can propagate on male and female Escherichia coli. We found that the growth patterns of phages T7M and T3 do not match the above characteristics, instead showing strain dependent male exclusion. Furthermore, a T3/7 hybrid phage exhibits a broader host range relative to that of T3, T7, as well as T7M, and is able to overcome the male exclusion. The T7M sequence closely resembles that of T3. T3/7 is essentially T3 based, but a DNA fragment containing part of the tail fiber gene 17 is replaced by the T7 sequence. T3 displays inferior adsorption to strains tested herein compared to T7. The T3 and T7 recombinant phage carries altered tail fibers and acquires better adsorption efficiency than T3. How phages T3 and T7 recombine was previously unclear. This study is the first to show that recombination can occur accurately within only 8 base-pair homology, where four-way junction structures are identified. Genomic recombination models based on endonuclease I cleavages at equivalent and nonequivalent sites followed by strand annealing are proposed. Retention of pseudo-palindromes can increase recombination frequency for reviving under stress. |
| first_indexed | 2024-12-13T04:02:25Z |
| format | Article |
| id | doaj.art-6b6f08b1ae7343aca4911be88d0d55b7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T04:02:25Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6b6f08b1ae7343aca4911be88d0d55b72022-12-22T00:00:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3095410.1371/journal.pone.0030954A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range.Tiao-Yin LinYi-Haw LoPin-Wei TsengShun-Fu ChangYann-Tsyr LinTon-Seng ChenIt is usually thought that bacteriophage T7 is female specific, while phage T3 can propagate on male and female Escherichia coli. We found that the growth patterns of phages T7M and T3 do not match the above characteristics, instead showing strain dependent male exclusion. Furthermore, a T3/7 hybrid phage exhibits a broader host range relative to that of T3, T7, as well as T7M, and is able to overcome the male exclusion. The T7M sequence closely resembles that of T3. T3/7 is essentially T3 based, but a DNA fragment containing part of the tail fiber gene 17 is replaced by the T7 sequence. T3 displays inferior adsorption to strains tested herein compared to T7. The T3 and T7 recombinant phage carries altered tail fibers and acquires better adsorption efficiency than T3. How phages T3 and T7 recombine was previously unclear. This study is the first to show that recombination can occur accurately within only 8 base-pair homology, where four-way junction structures are identified. Genomic recombination models based on endonuclease I cleavages at equivalent and nonequivalent sites followed by strand annealing are proposed. Retention of pseudo-palindromes can increase recombination frequency for reviving under stress.http://europepmc.org/articles/PMC3276506?pdf=render |
| spellingShingle | Tiao-Yin Lin Yi-Haw Lo Pin-Wei Tseng Shun-Fu Chang Yann-Tsyr Lin Ton-Seng Chen A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range. PLoS ONE |
| title | A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range. |
| title_full | A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range. |
| title_fullStr | A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range. |
| title_full_unstemmed | A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range. |
| title_short | A T3 and T7 recombinant phage acquires efficient adsorption and a broader host range. |
| title_sort | t3 and t7 recombinant phage acquires efficient adsorption and a broader host range |
| url | http://europepmc.org/articles/PMC3276506?pdf=render |
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