Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells
Monosodium glutamate induces excitotoxicity in the central nervous system through hyperactivation of both ionotropic and metabotropic glutamate receptors, which leads to neuronal cell death. In this study, we investigated the neuroprotective effects of naringenin on excitotoxicity induced b...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500023X.pdf |
| _version_ | 1818244117541421056 |
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| author | Xu Xiao-Hui Ma Cong-Min Han Yue-Zhen Li Yans Liu Chao Duan Zhi-Hui Wang Hui-Lin Liu De-Quan Liu Rui-Hua |
| author_facet | Xu Xiao-Hui Ma Cong-Min Han Yue-Zhen Li Yans Liu Chao Duan Zhi-Hui Wang Hui-Lin Liu De-Quan Liu Rui-Hua |
| author_sort | Xu Xiao-Hui |
| collection | DOAJ |
| description | Monosodium glutamate induces excitotoxicity in the central nervous system
through hyperactivation of both ionotropic and metabotropic glutamate
receptors, which leads to neuronal cell death. In this study, we investigated
the neuroprotective effects of naringenin on excitotoxicity induced by
glutamate in primary hippocampal neurons of neonatal mice. The expression
levels of apoptosis-inducing proteins and as well as ischemic factors were
observed by Western blot analysis. Immunocytochemistry and morphometric
analysis of hippocampal cells with or without glutamate and naringenin
treatment were performed. We observed that naringenin regulated Erk1/2 and
Akt phosphorylation and reduced the demise of dendrites due to glutamate
exposure in cultured hippocampal neurons. Furthermore, naringenin induced the
brain-derived neurotrophic factor and other neuroprotective cytokines, and
markedly improved the survival rates of the neurons 24 h following glutamate
exposure. The observed results suggest that the naturally occurring
bioflavonoid (naringenin) exerts neuroprotective effects via highly specific
molecular targets in neurons. |
| first_indexed | 2024-12-12T14:11:55Z |
| format | Article |
| id | doaj.art-6b6f3c88fa44499db89012d3ca6c1ae7 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-12T14:11:55Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-6b6f3c88fa44499db89012d3ca6c1ae72022-12-22T00:22:03ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-0167263964610.2298/ABS140811023X0354-46641500023XProtective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cellsXu Xiao-Hui0Ma Cong-Min1Han Yue-Zhen2Li Yans3Liu Chao4Duan Zhi-Hui5Wang Hui-Lin6Liu De-Quan7Liu Rui-Hua8Luoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaLuoyang Central Hospital Affiliated to Zhengzhou University, Department of Neurology, Luoyang, PR ChinaMonosodium glutamate induces excitotoxicity in the central nervous system through hyperactivation of both ionotropic and metabotropic glutamate receptors, which leads to neuronal cell death. In this study, we investigated the neuroprotective effects of naringenin on excitotoxicity induced by glutamate in primary hippocampal neurons of neonatal mice. The expression levels of apoptosis-inducing proteins and as well as ischemic factors were observed by Western blot analysis. Immunocytochemistry and morphometric analysis of hippocampal cells with or without glutamate and naringenin treatment were performed. We observed that naringenin regulated Erk1/2 and Akt phosphorylation and reduced the demise of dendrites due to glutamate exposure in cultured hippocampal neurons. Furthermore, naringenin induced the brain-derived neurotrophic factor and other neuroprotective cytokines, and markedly improved the survival rates of the neurons 24 h following glutamate exposure. The observed results suggest that the naturally occurring bioflavonoid (naringenin) exerts neuroprotective effects via highly specific molecular targets in neurons.http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500023X.pdfexcitotoxicityglutamatehippocampal neuronal cellsnaringeninneuroprotection |
| spellingShingle | Xu Xiao-Hui Ma Cong-Min Han Yue-Zhen Li Yans Liu Chao Duan Zhi-Hui Wang Hui-Lin Liu De-Quan Liu Rui-Hua Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells Archives of Biological Sciences excitotoxicity glutamate hippocampal neuronal cells naringenin neuroprotection |
| title | Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells |
| title_full | Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells |
| title_fullStr | Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells |
| title_full_unstemmed | Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells |
| title_short | Protective effect of naringenin on glutamate-induced neurotoxicity in cultured hippocampal cells |
| title_sort | protective effect of naringenin on glutamate induced neurotoxicity in cultured hippocampal cells |
| topic | excitotoxicity glutamate hippocampal neuronal cells naringenin neuroprotection |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500023X.pdf |
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