| Summary: | <p>Abstract</p> <p>Background</p> <p>The goal of the present study was the characterization of <it>Plasmodium falciparum </it>genes associated to malaria drug resistance (<it>pfcrt</it>, <it>pfdhfr </it>and <it>pfdhps)</it>, in samples from two Brazilian localities.</p> <p>Methods</p> <p>Parasites from 65 <it>P. falciparum </it>samples were genotyped using nested-PCR and direct DNA sequencing.</p> <p>Results</p> <p>Six resistant sulphadoxine-pyrimethamine (SP) <it>pfdhfr </it>genotypes and one haplotype associated to SP sensitivity were detected. For <it>pfcrt </it>gene, SVMNT chloroquine (CQ)-resistant genotype was detected as well as the CVMNK CQ-sensitive haplotype in the same sample from Paragominas, that showed a SP-sensitive genotype.</p> <p>Conclusion</p> <p>This study is the first to document the sensitivity of <it>P. falciparum </it>parasites to CQ and SP in Brazilian field samples. The importance of these findings is discussed.</p>
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