Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions
Limosilactobacillus reuteri produces reuterin via glycerol anaerobic fermentation. This compound has antimicrobial properties and is used for food preservation purposes. Filmogenic solutions constituted of polysaccharides and glycerol are also employed, however, reuterin synthesis in filmogenic solu...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927121001027 |
| _version_ | 1818352973299843072 |
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| author | F.J. Rodrigues M.F. Cedran J.L. Bicas H.H. Sato |
| author_facet | F.J. Rodrigues M.F. Cedran J.L. Bicas H.H. Sato |
| author_sort | F.J. Rodrigues |
| collection | DOAJ |
| description | Limosilactobacillus reuteri produces reuterin via glycerol anaerobic fermentation. This compound has antimicrobial properties and is used for food preservation purposes. Filmogenic solutions constituted of polysaccharides and glycerol are also employed, however, reuterin synthesis in filmogenic solutions has not yet been reported. Thus, the aim of this study was to optimize the in situ reuterin production by L. reuteri in alginate- and glycerol based-filmogenic solution, evaluating the survival of reuterin-producing bacteria during fermentation. The study consisted of a completely randomized design employing two L. reuteri strains (DSM 20016 and DSM 17938). The filmogenic solutions were obtained using sodium alginate (20 g/L) and two independent variables were studied: glycerol (0–300 mmol/L) and initial biomass of L. reuteri (≅6, 7, and 8 log CFU/mL). The samples were analyzed every 24 h for 72 h of anaerobic fermentation (37 °C). Both L. reuteri strains confirmed the potential for reuterin production and were susceptible to the metabolite produced. The highest reuterin production was achieved using L. reuteri DSM 20016. The initial microbial biomass of 8 log CFU/mL and 100 mmol/L of glycerol increased the reuterin production. However, higher conversion yields from glycerol to reuterin were obtained using 50 mmol/L of substrate. |
| first_indexed | 2024-12-13T19:02:08Z |
| format | Article |
| id | doaj.art-6b73bdefb9ac4be3badb586b1943a66e |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-12-13T19:02:08Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-6b73bdefb9ac4be3badb586b1943a66e2022-12-21T23:34:39ZengElsevierCurrent Research in Food Science2665-92712021-01-014926931Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutionsF.J. Rodrigues0M.F. Cedran1J.L. Bicas2H.H. Sato3Food Biochemistry Laboratory, Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, St. Monteiro Lobato 80, 13083-862, Campinas, SP, Brazil; Corresponding author.Food Biotechnology Laboratory, Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, St. Monteiro Lobato 80, 13083-862, Campinas, SP, BrazilFood Biotechnology Laboratory, Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, St. Monteiro Lobato 80, 13083-862, Campinas, SP, BrazilFood Biochemistry Laboratory, Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, St. Monteiro Lobato 80, 13083-862, Campinas, SP, BrazilLimosilactobacillus reuteri produces reuterin via glycerol anaerobic fermentation. This compound has antimicrobial properties and is used for food preservation purposes. Filmogenic solutions constituted of polysaccharides and glycerol are also employed, however, reuterin synthesis in filmogenic solutions has not yet been reported. Thus, the aim of this study was to optimize the in situ reuterin production by L. reuteri in alginate- and glycerol based-filmogenic solution, evaluating the survival of reuterin-producing bacteria during fermentation. The study consisted of a completely randomized design employing two L. reuteri strains (DSM 20016 and DSM 17938). The filmogenic solutions were obtained using sodium alginate (20 g/L) and two independent variables were studied: glycerol (0–300 mmol/L) and initial biomass of L. reuteri (≅6, 7, and 8 log CFU/mL). The samples were analyzed every 24 h for 72 h of anaerobic fermentation (37 °C). Both L. reuteri strains confirmed the potential for reuterin production and were susceptible to the metabolite produced. The highest reuterin production was achieved using L. reuteri DSM 20016. The initial microbial biomass of 8 log CFU/mL and 100 mmol/L of glycerol increased the reuterin production. However, higher conversion yields from glycerol to reuterin were obtained using 50 mmol/L of substrate.http://www.sciencedirect.com/science/article/pii/S26659271210010273-HydroxypropionaldehydeLactobacillus reuteriFilm-forming solutionIn situ reuterin production |
| spellingShingle | F.J. Rodrigues M.F. Cedran J.L. Bicas H.H. Sato Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions Current Research in Food Science 3-Hydroxypropionaldehyde Lactobacillus reuteri Film-forming solution In situ reuterin production |
| title | Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions |
| title_full | Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions |
| title_fullStr | Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions |
| title_full_unstemmed | Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions |
| title_short | Reuterin-producing Limosilactobacillus reuteri: Optimization of in situ reuterin production in alginate-based filmogenic solutions |
| title_sort | reuterin producing limosilactobacillus reuteri optimization of in situ reuterin production in alginate based filmogenic solutions |
| topic | 3-Hydroxypropionaldehyde Lactobacillus reuteri Film-forming solution In situ reuterin production |
| url | http://www.sciencedirect.com/science/article/pii/S2665927121001027 |
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