Progress in Soybean Genetic Transformation Over the Last Decade
Soybean is one of the important food, feed, and biofuel crops in the world. Soybean genome modification by genetic transformation has been carried out for trait improvement for more than 4 decades. However, compared to other major crops such as rice, soybean is still recalcitrant to genetic transfor...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.900318/full |
| _version_ | 1818238850080702464 |
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| author | Hu Xu Yong Guo Lijuan Qiu Yidong Ran |
| author_facet | Hu Xu Yong Guo Lijuan Qiu Yidong Ran |
| author_sort | Hu Xu |
| collection | DOAJ |
| description | Soybean is one of the important food, feed, and biofuel crops in the world. Soybean genome modification by genetic transformation has been carried out for trait improvement for more than 4 decades. However, compared to other major crops such as rice, soybean is still recalcitrant to genetic transformation, and transgenic soybean production has been hampered by limitations such as low transformation efficiency and genotype specificity, and prolonged and tedious protocols. The primary goal in soybean transformation over the last decade is to achieve high efficiency and genotype flexibility. Soybean transformation has been improved by modifying tissue culture conditions such as selection of explant types, adjustment of culture medium components and choice of selection reagents, as well as better understanding the transformation mechanisms of specific approaches such as Agrobacterium infection. Transgenesis-based breeding of soybean varieties with new traits is now possible by development of improved protocols. In this review, we summarize the developments in soybean genetic transformation to date, especially focusing on the progress made using Agrobacterium-mediated methods and biolistic methods over the past decade. We also discuss current challenges and future directions. |
| first_indexed | 2024-12-12T12:48:12Z |
| format | Article |
| id | doaj.art-6b74649481d646818a4996c4564b1a56 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T12:48:12Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6b74649481d646818a4996c4564b1a562022-12-22T00:24:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.900318900318Progress in Soybean Genetic Transformation Over the Last DecadeHu Xu0Yong Guo1Lijuan Qiu2Yidong Ran3Tianjin Genovo Biotechnology Co., Ltd., Tianjin, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaTianjin Genovo Biotechnology Co., Ltd., Tianjin, ChinaSoybean is one of the important food, feed, and biofuel crops in the world. Soybean genome modification by genetic transformation has been carried out for trait improvement for more than 4 decades. However, compared to other major crops such as rice, soybean is still recalcitrant to genetic transformation, and transgenic soybean production has been hampered by limitations such as low transformation efficiency and genotype specificity, and prolonged and tedious protocols. The primary goal in soybean transformation over the last decade is to achieve high efficiency and genotype flexibility. Soybean transformation has been improved by modifying tissue culture conditions such as selection of explant types, adjustment of culture medium components and choice of selection reagents, as well as better understanding the transformation mechanisms of specific approaches such as Agrobacterium infection. Transgenesis-based breeding of soybean varieties with new traits is now possible by development of improved protocols. In this review, we summarize the developments in soybean genetic transformation to date, especially focusing on the progress made using Agrobacterium-mediated methods and biolistic methods over the past decade. We also discuss current challenges and future directions.https://www.frontiersin.org/articles/10.3389/fpls.2022.900318/fullsoybean transformationtransformation efficiencygenotypeAgrobacteriumbiolistic methodgenome editing |
| spellingShingle | Hu Xu Yong Guo Lijuan Qiu Yidong Ran Progress in Soybean Genetic Transformation Over the Last Decade Frontiers in Plant Science soybean transformation transformation efficiency genotype Agrobacterium biolistic method genome editing |
| title | Progress in Soybean Genetic Transformation Over the Last Decade |
| title_full | Progress in Soybean Genetic Transformation Over the Last Decade |
| title_fullStr | Progress in Soybean Genetic Transformation Over the Last Decade |
| title_full_unstemmed | Progress in Soybean Genetic Transformation Over the Last Decade |
| title_short | Progress in Soybean Genetic Transformation Over the Last Decade |
| title_sort | progress in soybean genetic transformation over the last decade |
| topic | soybean transformation transformation efficiency genotype Agrobacterium biolistic method genome editing |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.900318/full |
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