Application of histochemical stains in anatomical research: A brief overview of the methods

Background: In anatomical research, there is often a need to present complex topographical relationships in more detail, under magnification, beyond the scope of gross anatomy. However, more than magnification is often needed to better understand the arrangement of small anatomical structures. Speci...

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Main Authors: Michał Golberg, Józef Kobos, Edward Clarke, Armand Bajaka, Anna Smędra, Krzysztof Balawender, Agata Wawrzyniak, Michał Seneczko, Stanisław Orkisz, Andrzej Żytkowski
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:Translational Research in Anatomy
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2214854X24000141
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author Michał Golberg
Józef Kobos
Edward Clarke
Armand Bajaka
Anna Smędra
Krzysztof Balawender
Agata Wawrzyniak
Michał Seneczko
Stanisław Orkisz
Andrzej Żytkowski
author_facet Michał Golberg
Józef Kobos
Edward Clarke
Armand Bajaka
Anna Smędra
Krzysztof Balawender
Agata Wawrzyniak
Michał Seneczko
Stanisław Orkisz
Andrzej Żytkowski
author_sort Michał Golberg
collection DOAJ
description Background: In anatomical research, there is often a need to present complex topographical relationships in more detail, under magnification, beyond the scope of gross anatomy. However, more than magnification is often needed to better understand the arrangement of small anatomical structures. Special stains allow for the differentiation of connective tissue stroma and mutual relations between small structures such as blood vessels and nerves. Aim: This review presents selected histochemical methods that can be applied in anatomical research. Such a concise outline can be both a compendium of knowledge for beginning researchers and an inspiration for anatomists and clinicians exploring the human body's details. Conclusions: The advantage of histochemical staining is its relatively low cost and the large amount of information that can be obtained with their use.
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spelling doaj.art-6b7833ce533f4946b04e3335a140b7632024-03-14T06:14:37ZengElsevierTranslational Research in Anatomy2214-854X2024-06-0135100294Application of histochemical stains in anatomical research: A brief overview of the methodsMichał Golberg0Józef Kobos1Edward Clarke2Armand Bajaka3Anna Smędra4Krzysztof Balawender5Agata Wawrzyniak6Michał Seneczko7Stanisław Orkisz8Andrzej Żytkowski9Department of Histology and Embryology, Medical University of Lodz, Łódź, Poland; Corresponding author. Department of Histology and Embryology, Medical University of Lodz, Poland.Department of Histology and Embryology, Medical University of Lodz, Łódź, PolandDepartment of Normal and Clinical Anatomy, Medical University of Lodz, Łódź, PolandDepartment of Child Psychiatry, Warsaw Medical University, Warsaw, PolandDepartment of Forensic Medicine, Medical Faculty Medical University of Lodz, Łódź, PolandDepartment of Normal and Clinical Anatomy, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszów, PolandDepartment of Histology and Embryology, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszów, PolandFaculty of Medicine, University of Social Sciences in Lodz, Łódź, PolandDepartment of Anatomy, Faculty of Medicine, University of Social Sciences in Lodz, Łódź, PolandDepartment of Anatomy, Faculty of Medicine, University of Social Sciences in Lodz, Łódź, Poland; Norbert Barlicki Memorial Teaching Hospital No. 1 of the Medical University of Lodz, Łódź, PolandBackground: In anatomical research, there is often a need to present complex topographical relationships in more detail, under magnification, beyond the scope of gross anatomy. However, more than magnification is often needed to better understand the arrangement of small anatomical structures. Special stains allow for the differentiation of connective tissue stroma and mutual relations between small structures such as blood vessels and nerves. Aim: This review presents selected histochemical methods that can be applied in anatomical research. Such a concise outline can be both a compendium of knowledge for beginning researchers and an inspiration for anatomists and clinicians exploring the human body's details. Conclusions: The advantage of histochemical staining is its relatively low cost and the large amount of information that can be obtained with their use.http://www.sciencedirect.com/science/article/pii/S2214854X24000141Histological stainingHistologyHistochemistryMicroscopical anatomyTissue processing
spellingShingle Michał Golberg
Józef Kobos
Edward Clarke
Armand Bajaka
Anna Smędra
Krzysztof Balawender
Agata Wawrzyniak
Michał Seneczko
Stanisław Orkisz
Andrzej Żytkowski
Application of histochemical stains in anatomical research: A brief overview of the methods
Translational Research in Anatomy
Histological staining
Histology
Histochemistry
Microscopical anatomy
Tissue processing
title Application of histochemical stains in anatomical research: A brief overview of the methods
title_full Application of histochemical stains in anatomical research: A brief overview of the methods
title_fullStr Application of histochemical stains in anatomical research: A brief overview of the methods
title_full_unstemmed Application of histochemical stains in anatomical research: A brief overview of the methods
title_short Application of histochemical stains in anatomical research: A brief overview of the methods
title_sort application of histochemical stains in anatomical research a brief overview of the methods
topic Histological staining
Histology
Histochemistry
Microscopical anatomy
Tissue processing
url http://www.sciencedirect.com/science/article/pii/S2214854X24000141
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