Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease
Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are un...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19490976.2021.1994833 |
| _version_ | 1818324075809865728 |
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| author | Zhilu Xu Xiangqian Dong Keli Yang Caroline Chevarin Jingwan Zhang Yu Lin Tao Zuo Lok Cheung Chu Yang Sun Fengrui Zhang Francis Kl Chan Joseph Jy Sung Jun Yu Anthony Buisson Nicolas Barnich Jean-Frédéric Colombel Sunny Hei Wong Yinglei Miao Siew C Ng |
| author_facet | Zhilu Xu Xiangqian Dong Keli Yang Caroline Chevarin Jingwan Zhang Yu Lin Tao Zuo Lok Cheung Chu Yang Sun Fengrui Zhang Francis Kl Chan Joseph Jy Sung Jun Yu Anthony Buisson Nicolas Barnich Jean-Frédéric Colombel Sunny Hei Wong Yinglei Miao Siew C Ng |
| author_sort | Zhilu Xu |
| collection | DOAJ |
| description | Adherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are unclear. AIEC presence was assessed in ileal tissues of 60 Hong Kong Chinese patients with CD and 56 healthy subjects. Mucosa microbiota was analyzed by 16s rRNA sequencing. Impact of AIEC on the gut microbiota was determined in a mouse model. AIEC was significantly more prevalent in ileal tissues of patients with CD than controls (30% vs 7.1%). Presence of AIEC in ileal tissues was associated with more severe mucosa microbiota dysbiosis in CD with decreased diversity and lower abundance of Firmicutes including butyrate producing Roseburia and probiotic Bacillus. A random forest model predicted the presence of AIEC with area under the curve of 0.89. AIEC exacerbated dysbiosis in dextran sodium sulfate (DSS)-induced colitis mice and led to resistance to restoration of normal gut microbiota by fecal microbiota transplantation (FMT). Proportion of donor-derived bacteria in AIEC-colonized mice was significantly lower than that in uninfected mice. AIEC was prevalent and associated with severe mucosa microbiota dysbiosis in CD in Hong Kong Chinese population. The presence of AIEC impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT. |
| first_indexed | 2024-12-13T11:22:49Z |
| format | Article |
| id | doaj.art-6b7c9b2d5c8b44a68d038f2a48442aa7 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-12-13T11:22:49Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-6b7c9b2d5c8b44a68d038f2a48442aa72022-12-21T23:48:24ZengTaylor & Francis GroupGut Microbes1949-09761949-09842021-01-0113110.1080/19490976.2021.19948331994833Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s diseaseZhilu Xu0Xiangqian Dong1Keli Yang2Caroline Chevarin3Jingwan Zhang4Yu Lin5Tao Zuo6Lok Cheung Chu7Yang Sun8Fengrui Zhang9Francis Kl Chan10Joseph Jy Sung11Jun Yu12Anthony Buisson13Nicolas Barnich14Jean-Frédéric Colombel15Sunny Hei Wong16Yinglei Miao17Siew C Ng18Institute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongThe First Affiliated Hospital of Kunming Medical UniversityInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongUniversité Clermont Auvergne, Inserm U1071, Usc-inrae 2018, Microbes, Intestin, Inflammation Et Susceptibilité De l’Hôte (M2ish)Institute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongThe First Affiliated Hospital of Kunming Medical UniversityThe First Affiliated Hospital of Kunming Medical UniversityInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongUniversité Clermont Auvergne, Inserm U1071, Usc-inrae 2018, Microbes, Intestin, Inflammation Et Susceptibilité De l’Hôte (M2ish)Université Clermont Auvergne, Inserm U1071, Usc-inrae 2018, Microbes, Intestin, Inflammation Et Susceptibilité De l’Hôte (M2ish)Icahn School of MedicineInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongThe First Affiliated Hospital of Kunming Medical UniversityInstitute of Digestive Disease, State Key Laboratory of Digestive Diseases, Lks Institute of Health Science, the Chinese University of Hong KongAdherent invasive Escherichia Coli (AIEC) has been implicated in the pathogenesis of Crohn’s disease (CD) in Western populations. Whether the presence of AIEC is also seen in CD populations of different genetic susceptibility and has negative impact on host microbiota ecology and therapeutics are unclear. AIEC presence was assessed in ileal tissues of 60 Hong Kong Chinese patients with CD and 56 healthy subjects. Mucosa microbiota was analyzed by 16s rRNA sequencing. Impact of AIEC on the gut microbiota was determined in a mouse model. AIEC was significantly more prevalent in ileal tissues of patients with CD than controls (30% vs 7.1%). Presence of AIEC in ileal tissues was associated with more severe mucosa microbiota dysbiosis in CD with decreased diversity and lower abundance of Firmicutes including butyrate producing Roseburia and probiotic Bacillus. A random forest model predicted the presence of AIEC with area under the curve of 0.89. AIEC exacerbated dysbiosis in dextran sodium sulfate (DSS)-induced colitis mice and led to resistance to restoration of normal gut microbiota by fecal microbiota transplantation (FMT). Proportion of donor-derived bacteria in AIEC-colonized mice was significantly lower than that in uninfected mice. AIEC was prevalent and associated with severe mucosa microbiota dysbiosis in CD in Hong Kong Chinese population. The presence of AIEC impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT.http://dx.doi.org/10.1080/19490976.2021.1994833adherent-invasive escherichia coliaieccrohn’s diseaseinflammatory bowel diseasemicrobiota |
| spellingShingle | Zhilu Xu Xiangqian Dong Keli Yang Caroline Chevarin Jingwan Zhang Yu Lin Tao Zuo Lok Cheung Chu Yang Sun Fengrui Zhang Francis Kl Chan Joseph Jy Sung Jun Yu Anthony Buisson Nicolas Barnich Jean-Frédéric Colombel Sunny Hei Wong Yinglei Miao Siew C Ng Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease Gut Microbes adherent-invasive escherichia coli aiec crohn’s disease inflammatory bowel disease microbiota |
| title | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_full | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_fullStr | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_full_unstemmed | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_short | Association of Adherent-invasive Escherichia coli with severe Gut Mucosal dysbiosis in Hong Kong Chinese population with Crohn’s disease |
| title_sort | association of adherent invasive escherichia coli with severe gut mucosal dysbiosis in hong kong chinese population with crohn s disease |
| topic | adherent-invasive escherichia coli aiec crohn’s disease inflammatory bowel disease microbiota |
| url | http://dx.doi.org/10.1080/19490976.2021.1994833 |
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