| Summary: | <i>Solidago rugosa</i> is one of the goldenrod species native to North America but has sporadically naturalized as an alien plant in Europe. The investigation of the root and leaf ethanol extracts of the plant using a bioassay-guided process with an anti-<i>Bacillus</i> assay resulted in the isolation of two antimicrobial components. Structure elucidation was performed based on high-resolution tandem mass spectrometric and one- and two-dimensional NMR spectroscopic analyses that revealed (–)-hardwickiic acid (Compound <b>1</b>) and (–)-abietic acid (Compound <b>2</b>). The isolates were evaluated for their antimicrobial properties against several plant pathogenic bacterial and fungal strains. Both compounds demonstrated an antibacterial effect, especially against Gram-positive bacterial strains (<i>Bacillus spizizenii</i>, <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>, and <i>Curtobacterium flaccumfaciens</i> pv. <i>flaccumfaciens</i>) with half maximal inhibitory concentration (IC<sub>50</sub>) between 1 and 5.1 µg/mL (5–20 times higher than that of the positive control gentamicin). In the used concentrations, minimal bactericidal concentration (MBC) was reached only against the non-pathogen <i>B. spizizenii</i>. Besides their activity against <i>Fusarium avenaceum</i>, the highest antifungal activity was observed for Compound <b>1</b> against <i>Bipolaris sorokiniana</i> with an IC<sub>50</sub> of 3.8 µg/mL.
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