Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression
Androgen exerts its functions by binding with an androgen receptor (AR). It can activate many signaling pathways that are important to the progression of castration-resistant prostate cancer (CRPC). Here, we characterized the rapid proteomic changes seen at 5, 15, 30, and 60 min after the androgen t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/12/1877 |
| _version_ | 1797506514264522752 |
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| author | Jong-Kwang Kim Jae-Hun Jung Dong-Hoon Shin Hye-Jin You Seho Cha Bo-Seul Song Jae-Young Joung Weon-Seo Park Kwang-Pyo Kim Jae-Kyung Myung |
| author_facet | Jong-Kwang Kim Jae-Hun Jung Dong-Hoon Shin Hye-Jin You Seho Cha Bo-Seul Song Jae-Young Joung Weon-Seo Park Kwang-Pyo Kim Jae-Kyung Myung |
| author_sort | Jong-Kwang Kim |
| collection | DOAJ |
| description | Androgen exerts its functions by binding with an androgen receptor (AR). It can activate many signaling pathways that are important to the progression of castration-resistant prostate cancer (CRPC). Here, we characterized the rapid proteomic changes seen at 5, 15, 30, and 60 min after the androgen treatment of VCaP cells via the tandem mass tag (TMT) labeling strategy. A total of 5529 proteins were successfully identified and quantified. Dynamic time profiling of protein expression patterns allowed us to identify five protein clusters involved in various stages of androgen-initiated signal transmission and processing. More details of protein functions and localization patterns, and our elucidation of an AR-interacting protein network, were obtained. Finally, we validated the expression level of AR-regulated proteins known to be significantly regulated in CRPC patients using the mouse xenograft model and patient samples. Our work offers a systematic analysis of the rapid proteomic changes induced by androgen and provides a global view of the molecular mechanisms underlying CRPC progression. |
| first_indexed | 2024-03-10T04:33:43Z |
| format | Article |
| id | doaj.art-6b7e7550bec8479da890a933ba610b2f |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T04:33:43Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-6b7e7550bec8479da890a933ba610b2f2023-11-23T03:56:57ZengMDPI AGBiomedicines2227-90592021-12-01912187710.3390/biomedicines9121877Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer ProgressionJong-Kwang Kim0Jae-Hun Jung1Dong-Hoon Shin2Hye-Jin You3Seho Cha4Bo-Seul Song5Jae-Young Joung6Weon-Seo Park7Kwang-Pyo Kim8Jae-Kyung Myung9Research Core Center, National Cancer Center, Goyang-si 10408, KoreaDepartment of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin-si 17104, KoreaDepartment of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang-si 10408, KoreaDepartment of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang-si 10408, KoreaDepartment of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang-si 10408, KoreaDepartment of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang-si 10408, KoreaDepartment of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang-si 10408, KoreaDepartment of Pathology, National Cancer Center, Goyang-si 10408, KoreaDepartment of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin-si 17104, KoreaDepartment of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang-si 10408, KoreaAndrogen exerts its functions by binding with an androgen receptor (AR). It can activate many signaling pathways that are important to the progression of castration-resistant prostate cancer (CRPC). Here, we characterized the rapid proteomic changes seen at 5, 15, 30, and 60 min after the androgen treatment of VCaP cells via the tandem mass tag (TMT) labeling strategy. A total of 5529 proteins were successfully identified and quantified. Dynamic time profiling of protein expression patterns allowed us to identify five protein clusters involved in various stages of androgen-initiated signal transmission and processing. More details of protein functions and localization patterns, and our elucidation of an AR-interacting protein network, were obtained. Finally, we validated the expression level of AR-regulated proteins known to be significantly regulated in CRPC patients using the mouse xenograft model and patient samples. Our work offers a systematic analysis of the rapid proteomic changes induced by androgen and provides a global view of the molecular mechanisms underlying CRPC progression.https://www.mdpi.com/2227-9059/9/12/1877castration resistant prostate cancerandrogen receptorrapid signalingproteomicsmass spectrometrybioinformatics |
| spellingShingle | Jong-Kwang Kim Jae-Hun Jung Dong-Hoon Shin Hye-Jin You Seho Cha Bo-Seul Song Jae-Young Joung Weon-Seo Park Kwang-Pyo Kim Jae-Kyung Myung Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression Biomedicines castration resistant prostate cancer androgen receptor rapid signaling proteomics mass spectrometry bioinformatics |
| title | Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression |
| title_full | Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression |
| title_fullStr | Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression |
| title_full_unstemmed | Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression |
| title_short | Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression |
| title_sort | rapid androgen responsive proteome is involved in prostate cancer progression |
| topic | castration resistant prostate cancer androgen receptor rapid signaling proteomics mass spectrometry bioinformatics |
| url | https://www.mdpi.com/2227-9059/9/12/1877 |
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