Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus
A promoter tagging program in the legume Lotus japonicus was initiated to identify plant genes involved in the nitrogen-fixing symbiosis between legumes and rhizobia. Seven transformed plant lines expressing the promoterless reporter gene uidA (β-glucuronidase; GUS) specifically in roots and/or nodu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2000-06-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.6.606 |
| _version_ | 1828739355206746112 |
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| author | K. Judith Webb Leif Skøt Margaret N. Nicholson Bodil Jørgensen Sue Mizen |
| author_facet | K. Judith Webb Leif Skøt Margaret N. Nicholson Bodil Jørgensen Sue Mizen |
| author_sort | K. Judith Webb |
| collection | DOAJ |
| description | A promoter tagging program in the legume Lotus japonicus was initiated to identify plant genes involved in the nitrogen-fixing symbiosis between legumes and rhizobia. Seven transformed plant lines expressing the promoterless reporter gene uidA (β-glucuronidase; GUS) specifically in roots and/or nodules were identified. Four of these expressed GUS in the roots only after inoculation with nodule-forming Mesorhizobium loti. In one line (T90), GUS activity was found in the root epidermis, including root hairs. During seedling growth, GUS expression gradually became focused in developing nodules and disappeared from root tissue. No GUS activity was detected when a non-nodulating mutant of M. loti was used to inoculate the plants. The T-DNA insertion in this plant line was located 1.3 kb upstream of a putative coding sequence with strong homology to calcium-binding proteins. Four motifs were identified, which were very similar to the “EF hands” in calmodulin-related proteins, each binding one Ca2+. We have named the gene LjCbp1 (calcium-binding protein). Northern (RNA) analyses showed that this gene is expressed specifically in roots of L. japonicus. Expression was reduced in roots inoculated with non-nodulating M. loti mutants and in progeny homozygous for the T-DNA insertion, suggesting a link between the T-DNA insertion and this gene. |
| first_indexed | 2024-04-13T00:21:15Z |
| format | Article |
| id | doaj.art-6b7f874858614304bc858bac2ed6d0b5 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:21:15Z |
| publishDate | 2000-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-6b7f874858614304bc858bac2ed6d0b52022-12-22T03:10:45ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-06-0113660661610.1094/MPMI.2000.13.6.606Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicusK. Judith WebbLeif SkøtMargaret N. NicholsonBodil JørgensenSue MizenA promoter tagging program in the legume Lotus japonicus was initiated to identify plant genes involved in the nitrogen-fixing symbiosis between legumes and rhizobia. Seven transformed plant lines expressing the promoterless reporter gene uidA (β-glucuronidase; GUS) specifically in roots and/or nodules were identified. Four of these expressed GUS in the roots only after inoculation with nodule-forming Mesorhizobium loti. In one line (T90), GUS activity was found in the root epidermis, including root hairs. During seedling growth, GUS expression gradually became focused in developing nodules and disappeared from root tissue. No GUS activity was detected when a non-nodulating mutant of M. loti was used to inoculate the plants. The T-DNA insertion in this plant line was located 1.3 kb upstream of a putative coding sequence with strong homology to calcium-binding proteins. Four motifs were identified, which were very similar to the “EF hands” in calmodulin-related proteins, each binding one Ca2+. We have named the gene LjCbp1 (calcium-binding protein). Northern (RNA) analyses showed that this gene is expressed specifically in roots of L. japonicus. Expression was reduced in roots inoculated with non-nodulating M. loti mutants and in progeny homozygous for the T-DNA insertion, suggesting a link between the T-DNA insertion and this gene.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.6.606Agrobacterium tumefaciens |
| spellingShingle | K. Judith Webb Leif Skøt Margaret N. Nicholson Bodil Jørgensen Sue Mizen Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus Molecular Plant-Microbe Interactions Agrobacterium tumefaciens |
| title | Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus |
| title_full | Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus |
| title_fullStr | Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus |
| title_full_unstemmed | Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus |
| title_short | Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus |
| title_sort | mesorhizobium loti increases root specific expression of a calcium binding protein homologue identified by promoter tagging in lotus japonicus |
| topic | Agrobacterium tumefaciens |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.6.606 |
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