A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor
Due to the SARS-CoV-2 pandemic, there has been an increase in the search for affordable healthcare devices for mass testing and rapid diagnosis. In this context, this work described a new methodology for SARS-CoV-2 detection based on an impedimetric immunosensor developed using the advantageous immo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/15/4230 |
| _version_ | 1797525381379522560 |
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| author | Gabriel C. Zaccariotto Martin K. L. Silva Giovanna S. Rocha Ivana Cesarino |
| author_facet | Gabriel C. Zaccariotto Martin K. L. Silva Giovanna S. Rocha Ivana Cesarino |
| author_sort | Gabriel C. Zaccariotto |
| collection | DOAJ |
| description | Due to the SARS-CoV-2 pandemic, there has been an increase in the search for affordable healthcare devices for mass testing and rapid diagnosis. In this context, this work described a new methodology for SARS-CoV-2 detection based on an impedimetric immunosensor developed using the advantageous immobilization of antibodies in the reduced graphene oxide (rGO). The rGO was obtained by chemical synthesis from the commercial graphene oxide (GO), and the materials were morphologically, electrochemically and visually characterized. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were used to evaluate the fabrication steps of the immunosensor. The electrochemical immunoassay was considered for SARS-CoV-2 spike protein RBD detection using a impedimetric immunosensor and redox couple ([(Fe(CN)<sub>6</sub>)]<sup>3−/4−</sup>) as a probe. The immunosensor was effectively developed and applied in the detection of SARS-CoV-2 spike protein RBD in saliva samples. |
| first_indexed | 2024-03-10T09:12:03Z |
| format | Article |
| id | doaj.art-6b826660da0d41adbc8cb83a2d12077e |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T09:12:03Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-6b826660da0d41adbc8cb83a2d12077e2023-11-22T05:53:38ZengMDPI AGMaterials1996-19442021-07-011415423010.3390/ma14154230A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric ImmunosensorGabriel C. Zaccariotto0Martin K. L. Silva1Giovanna S. Rocha2Ivana Cesarino3Department of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilDepartment of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilDepartment of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilDepartment of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilDue to the SARS-CoV-2 pandemic, there has been an increase in the search for affordable healthcare devices for mass testing and rapid diagnosis. In this context, this work described a new methodology for SARS-CoV-2 detection based on an impedimetric immunosensor developed using the advantageous immobilization of antibodies in the reduced graphene oxide (rGO). The rGO was obtained by chemical synthesis from the commercial graphene oxide (GO), and the materials were morphologically, electrochemically and visually characterized. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were used to evaluate the fabrication steps of the immunosensor. The electrochemical immunoassay was considered for SARS-CoV-2 spike protein RBD detection using a impedimetric immunosensor and redox couple ([(Fe(CN)<sub>6</sub>)]<sup>3−/4−</sup>) as a probe. The immunosensor was effectively developed and applied in the detection of SARS-CoV-2 spike protein RBD in saliva samples.https://www.mdpi.com/1996-1944/14/15/4230electrochemical sensorreduced graphene oxideCOVID-19SARS-CoV-2 |
| spellingShingle | Gabriel C. Zaccariotto Martin K. L. Silva Giovanna S. Rocha Ivana Cesarino A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor Materials electrochemical sensor reduced graphene oxide COVID-19 SARS-CoV-2 |
| title | A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor |
| title_full | A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor |
| title_fullStr | A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor |
| title_full_unstemmed | A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor |
| title_short | A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor |
| title_sort | novel method for the detection of sars cov 2 based on graphene impedimetric immunosensor |
| topic | electrochemical sensor reduced graphene oxide COVID-19 SARS-CoV-2 |
| url | https://www.mdpi.com/1996-1944/14/15/4230 |
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