Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells
Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2021-03-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-202103-0003_ubiquitin-specific-peptidase-8-regulates-proliferation-and-early-differentiation-of-sheep-skeletal-muscle-satel.php |
| _version_ | 1797899127623778304 |
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| author | Shaoyu Wang Kui Li Hui Gao Zepeng Liu Shuang Shi Qiang Tan Zhengguang Wang |
| author_facet | Shaoyu Wang Kui Li Hui Gao Zepeng Liu Shuang Shi Qiang Tan Zhengguang Wang |
| author_sort | Shaoyu Wang |
| collection | DOAJ |
| description | Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferation and differentiation of sheep skeletal muscle satellite cells. The results showed that the expression level of USP8 was significantly increased on days 2 and 3 following the induction of the differentiation process. Furthermore, knocking down USP8 resulted in a significant increase in myogenin-positive cells, and promoted early differentiation of satellite cells by regulating the expression level of paired box 7 (PAX7). Additionally, knocking down USP8 suppressed muscle satellite cell proliferation, possibly explaining that the relative mRNA level of USP8 was linearly related to muscle fibre density of Hu sheep. Overall, our research demonstrates that USP8 plays a role in proliferation and early differentiation of skeletal muscle satellite cells. |
| first_indexed | 2024-04-10T08:23:51Z |
| format | Article |
| id | doaj.art-6b853de470884a3b9d18109aa7f8a56d |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:23:51Z |
| publishDate | 2021-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-6b853de470884a3b9d18109aa7f8a56d2023-02-23T03:33:51ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092021-03-01663879610.17221/105/2020-CJAScjs-202103-0003Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cellsShaoyu Wang0Kui Li1Hui Gao2Zepeng Liu3Shuang Shi4Qiang Tan5Zhengguang Wang6College of Animal Sciences, Zhejiang University, Hangzhou, P.R. ChinaZhejiang Animal Husbandry Techniques Extension Station, Hangzhou, P.R. ChinaZhejiang Animal Husbandry Techniques Extension Station, Hangzhou, P.R. ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, P.R. ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, P.R. ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, P.R. ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, P.R. ChinaUbiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferation and differentiation of sheep skeletal muscle satellite cells. The results showed that the expression level of USP8 was significantly increased on days 2 and 3 following the induction of the differentiation process. Furthermore, knocking down USP8 resulted in a significant increase in myogenin-positive cells, and promoted early differentiation of satellite cells by regulating the expression level of paired box 7 (PAX7). Additionally, knocking down USP8 suppressed muscle satellite cell proliferation, possibly explaining that the relative mRNA level of USP8 was linearly related to muscle fibre density of Hu sheep. Overall, our research demonstrates that USP8 plays a role in proliferation and early differentiation of skeletal muscle satellite cells.https://cjas.agriculturejournals.cz/artkey/cjs-202103-0003_ubiquitin-specific-peptidase-8-regulates-proliferation-and-early-differentiation-of-sheep-skeletal-muscle-satel.phpmyogenesismyogenindesminmyosin heavy chain 3pax7 |
| spellingShingle | Shaoyu Wang Kui Li Hui Gao Zepeng Liu Shuang Shi Qiang Tan Zhengguang Wang Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells Czech Journal of Animal Science myogenesis myogenin desmin myosin heavy chain 3 pax7 |
| title | Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells |
| title_full | Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells |
| title_fullStr | Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells |
| title_full_unstemmed | Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells |
| title_short | Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells |
| title_sort | ubiquitin specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells |
| topic | myogenesis myogenin desmin myosin heavy chain 3 pax7 |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-202103-0003_ubiquitin-specific-peptidase-8-regulates-proliferation-and-early-differentiation-of-sheep-skeletal-muscle-satel.php |
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