Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Abstract Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, whic...

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Main Authors: Weiyu Fu, Xuehua Liao, Qian Zhang, Yuzhen Zhu, Si Mei, Qian Li, Xin Zhou, Xiaojun Li, Hui Luo, Hua Ye, Kefeng Wu
Format: Article
Language:English
Published: BMC 2022-06-01
Series:BMC Complementary Medicine and Therapies
Subjects:
Online Access:https://doi.org/10.1186/s12906-022-03646-6
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author Weiyu Fu
Xuehua Liao
Qian Zhang
Yuzhen Zhu
Si Mei
Qian Li
Xin Zhou
Xiaojun Li
Hui Luo
Hua Ye
Kefeng Wu
author_facet Weiyu Fu
Xuehua Liao
Qian Zhang
Yuzhen Zhu
Si Mei
Qian Li
Xin Zhou
Xiaojun Li
Hui Luo
Hua Ye
Kefeng Wu
author_sort Weiyu Fu
collection DOAJ
description Abstract Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical characteristics and the anti-melanogenesis effect of pectin extracted from wampee fruit in A375 cells. Methods The physicochemical characterization of pectin from wampee fruit was investigated by gel chromatography (GCP), FT-IR spectroscopy, and NMR spectroscopy methods. The anti-melanogenesis effects and mechanism were evaluated by mushroom tyrosine enzyme and human melanin cell model in vitro. Results The results showed that a molecular weight of 5.271 × 105 Da wampee fruit pectin (WFP) were mainly composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galacturonic acid (Gal A), galactose (Gal), and arabinose (Ara), which linked with →4)-β-D-Galp-(1 → units. The current study revealed that WFP could significantly suppress mushroom TRY activity in vitro. Furtherly, WFP significantly reduced intracellular and extracellular melanin formation in A375 melanoma cells depending on the presence of alpha-melanocyte stimulating hormone (α-MSH). TRY activity was only inhibited in α-MSH treated A375 cells. Western blot analysis demonstrated that WFP reverse α-MSH induced melanogenesis in A375 melanoma cells, including in down-regulated TRY, TYRP-1, TYRP-2, MITF and CREB expressions. Conclusion These results indicated that WFP could inhibit α-MSH induced melanogenesis in A375 melanoma cells via α-MSH/TRY pathway. In conclusion, these data provided a new perspective to annotate WFP anti-melanogenesis activity mechanism.
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spelling doaj.art-6b85705bba3a4f0ba53416d2f05c0dfa2022-12-22T03:38:16ZengBMCBMC Complementary Medicine and Therapies2662-76712022-06-0122111310.1186/s12906-022-03646-6Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cellsWeiyu Fu0Xuehua Liao1Qian Zhang2Yuzhen Zhu3Si Mei4Qian Li5Xin Zhou6Xiaojun Li7Hui Luo8Hua Ye9Kefeng Wu10Marine Biomedical Research Institution, Guangdong Medical UniversityGuangdong (Zhanjiang) provincial laboratory of Southern Marine Science and EngineeringGuangdong (Zhanjiang) provincial laboratory of Southern Marine Science and EngineeringGuangdong (Zhanjiang) provincial laboratory of Southern Marine Science and EngineeringMarine Biomedical Research Institution, Guangdong Medical UniversityMarine Biomedical Research Institution, Guangdong Medical UniversityGuangdong (Zhanjiang) provincial laboratory of Southern Marine Science and EngineeringBoRui Saccharide Biotech Co. LtdMarine Biomedical Research Institution, Guangdong Medical UniversityMarine Biomedical Research Institution, Guangdong Medical UniversityMarine Biomedical Research Institution, Guangdong Medical UniversityAbstract Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical characteristics and the anti-melanogenesis effect of pectin extracted from wampee fruit in A375 cells. Methods The physicochemical characterization of pectin from wampee fruit was investigated by gel chromatography (GCP), FT-IR spectroscopy, and NMR spectroscopy methods. The anti-melanogenesis effects and mechanism were evaluated by mushroom tyrosine enzyme and human melanin cell model in vitro. Results The results showed that a molecular weight of 5.271 × 105 Da wampee fruit pectin (WFP) were mainly composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galacturonic acid (Gal A), galactose (Gal), and arabinose (Ara), which linked with →4)-β-D-Galp-(1 → units. The current study revealed that WFP could significantly suppress mushroom TRY activity in vitro. Furtherly, WFP significantly reduced intracellular and extracellular melanin formation in A375 melanoma cells depending on the presence of alpha-melanocyte stimulating hormone (α-MSH). TRY activity was only inhibited in α-MSH treated A375 cells. Western blot analysis demonstrated that WFP reverse α-MSH induced melanogenesis in A375 melanoma cells, including in down-regulated TRY, TYRP-1, TYRP-2, MITF and CREB expressions. Conclusion These results indicated that WFP could inhibit α-MSH induced melanogenesis in A375 melanoma cells via α-MSH/TRY pathway. In conclusion, these data provided a new perspective to annotate WFP anti-melanogenesis activity mechanism.https://doi.org/10.1186/s12906-022-03646-6Wampee fruit pectinPhysicochemical characterizationAnti-melanogenesisα-MSH/TRY pathway
spellingShingle Weiyu Fu
Xuehua Liao
Qian Zhang
Yuzhen Zhu
Si Mei
Qian Li
Xin Zhou
Xiaojun Li
Hui Luo
Hua Ye
Kefeng Wu
Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells
BMC Complementary Medicine and Therapies
Wampee fruit pectin
Physicochemical characterization
Anti-melanogenesis
α-MSH/TRY pathway
title Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells
title_full Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells
title_fullStr Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells
title_full_unstemmed Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells
title_short Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells
title_sort anti melanogenesis effect from wampee fruit pectin via α msh try pathway in a375 cells
topic Wampee fruit pectin
Physicochemical characterization
Anti-melanogenesis
α-MSH/TRY pathway
url https://doi.org/10.1186/s12906-022-03646-6
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