Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia
Abstract Background Syringomyelia is a spinal cord cavity containing cerebrospinal fluid (CSF)-like fluid. If syringomyelia asymmetrically involves the dorsal horn grey matter of the spinal cord, affected dogs show increased signs of dysesthesia and neuropathic pain, like increased itching behaviour...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-023-03817-8 |
| _version_ | 1797452238635925504 |
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| author | Laura Lemke Regina Carlson Thomas Flegel Andrea Volk Holger Andreas Volk Andrea Tipold Jasmin Nicole Nessler |
| author_facet | Laura Lemke Regina Carlson Thomas Flegel Andrea Volk Holger Andreas Volk Andrea Tipold Jasmin Nicole Nessler |
| author_sort | Laura Lemke |
| collection | DOAJ |
| description | Abstract Background Syringomyelia is a spinal cord cavity containing cerebrospinal fluid (CSF)-like fluid. If syringomyelia asymmetrically involves the dorsal horn grey matter of the spinal cord, affected dogs show increased signs of dysesthesia and neuropathic pain, like increased itching behaviour. In the dorsal horn, amongst others, receptors for Interleukin-31 (IL-31) can be found. IL-31 is one of the main cytokines involved in the pathogenesis of pruritus in atopic dermatitis in different species. This study investigates suspected elevated levels of IL-31 in serum and CSF of dogs showing signs of pain or increased itching behaviour related to syringomyelia. The IL-31 were measured in archived samples (52 serum and 35 CSF samples) of dogs with syringomyelia (n = 48), atopic dermatitis (n = 3) and of healthy control dogs (n = 11) using a competitive canine IL-31 ELISA. Results Mean serum IL-31 level in dogs with syringomyelia was 150.1 pg/ml (n = 39), in dogs with atopic dermatitis 228.3 pg/ml (n = 3) and in healthy dogs 80.7 pg/ml (n = 10). Mean CSF IL-31 value was 146.3 pg/ml (n = 27) in dogs with syringomyelia and 186.2 pg/ml (n = 8) in healthy dogs. Individual patients with syringomyelia (especially dogs with otitis media or otitis media and interna or intervertebral disc herniation) showed high IL-31 levels in serum and CSF samples, but the difference was not statistically significant. IL-31 serum and CSF levels did not differ significantly in dogs with syringomyelia with or without itching behaviour and with or without signs of pain. Conclusion Based on this study, increased IL-31 levels seem not to be correlated with itching behaviour or signs of pain in dogs with syringomyelia, but might be caused by other underlying diseases. |
| first_indexed | 2024-03-09T15:05:54Z |
| format | Article |
| id | doaj.art-6b8846fc545d460f91bc949995811194 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-09T15:05:54Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-6b8846fc545d460f91bc9499958111942023-11-26T13:38:41ZengBMCBMC Veterinary Research1746-61482023-11-0119111210.1186/s12917-023-03817-8Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyeliaLaura Lemke0Regina Carlson1Thomas Flegel2Andrea Volk3Holger Andreas Volk4Andrea Tipold5Jasmin Nicole Nessler6Department of Small Animal Medicine and Surgery, University of Veterinary Medicine HannoverDepartment of Small Animal Medicine and Surgery, University of Veterinary Medicine HannoverDepartment of Small Animal Medicine, Leipzig UniversityDepartment of Small Animal Medicine and Surgery, University of Veterinary Medicine HannoverDepartment of Small Animal Medicine and Surgery, University of Veterinary Medicine HannoverDepartment of Small Animal Medicine and Surgery, University of Veterinary Medicine HannoverDepartment of Small Animal Medicine and Surgery, University of Veterinary Medicine HannoverAbstract Background Syringomyelia is a spinal cord cavity containing cerebrospinal fluid (CSF)-like fluid. If syringomyelia asymmetrically involves the dorsal horn grey matter of the spinal cord, affected dogs show increased signs of dysesthesia and neuropathic pain, like increased itching behaviour. In the dorsal horn, amongst others, receptors for Interleukin-31 (IL-31) can be found. IL-31 is one of the main cytokines involved in the pathogenesis of pruritus in atopic dermatitis in different species. This study investigates suspected elevated levels of IL-31 in serum and CSF of dogs showing signs of pain or increased itching behaviour related to syringomyelia. The IL-31 were measured in archived samples (52 serum and 35 CSF samples) of dogs with syringomyelia (n = 48), atopic dermatitis (n = 3) and of healthy control dogs (n = 11) using a competitive canine IL-31 ELISA. Results Mean serum IL-31 level in dogs with syringomyelia was 150.1 pg/ml (n = 39), in dogs with atopic dermatitis 228.3 pg/ml (n = 3) and in healthy dogs 80.7 pg/ml (n = 10). Mean CSF IL-31 value was 146.3 pg/ml (n = 27) in dogs with syringomyelia and 186.2 pg/ml (n = 8) in healthy dogs. Individual patients with syringomyelia (especially dogs with otitis media or otitis media and interna or intervertebral disc herniation) showed high IL-31 levels in serum and CSF samples, but the difference was not statistically significant. IL-31 serum and CSF levels did not differ significantly in dogs with syringomyelia with or without itching behaviour and with or without signs of pain. Conclusion Based on this study, increased IL-31 levels seem not to be correlated with itching behaviour or signs of pain in dogs with syringomyelia, but might be caused by other underlying diseases.https://doi.org/10.1186/s12917-023-03817-8Interleukin-31SyringomyeliaIL-31Air-scratchingCerebrospinal fluidSerum |
| spellingShingle | Laura Lemke Regina Carlson Thomas Flegel Andrea Volk Holger Andreas Volk Andrea Tipold Jasmin Nicole Nessler Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia BMC Veterinary Research Interleukin-31 Syringomyelia IL-31 Air-scratching Cerebrospinal fluid Serum |
| title | Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia |
| title_full | Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia |
| title_fullStr | Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia |
| title_full_unstemmed | Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia |
| title_short | Interleukin-31 in serum and cerebrospinal fluid of dogs with syringomyelia |
| title_sort | interleukin 31 in serum and cerebrospinal fluid of dogs with syringomyelia |
| topic | Interleukin-31 Syringomyelia IL-31 Air-scratching Cerebrospinal fluid Serum |
| url | https://doi.org/10.1186/s12917-023-03817-8 |
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