Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis
<i>Bruguiera gymnorhiza</i> (L.) Lam. is claimed to effectively manage a number of ailments including diabetes and associated complications. Nonetheless, no attempt has been made to delineate its pharmacological propensities and phytochemical profile. This study was designed to appraise...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/731 |
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| author | Nabeelah Bibi Sadeer Kouadio Ibrahime Sinan Zoltán Cziáky József Jekő Gokhan Zengin Rajesh Jeewon Hassan H. Abdallah Kannan R. R. Rengasamy Mohamad Fawzi Mahomoodally |
| author_facet | Nabeelah Bibi Sadeer Kouadio Ibrahime Sinan Zoltán Cziáky József Jekő Gokhan Zengin Rajesh Jeewon Hassan H. Abdallah Kannan R. R. Rengasamy Mohamad Fawzi Mahomoodally |
| author_sort | Nabeelah Bibi Sadeer |
| collection | DOAJ |
| description | <i>Bruguiera gymnorhiza</i> (L.) Lam. is claimed to effectively manage a number of ailments including diabetes and associated complications. Nonetheless, no attempt has been made to delineate its pharmacological propensities and phytochemical profile. This study was designed to appraise the antioxidant and enzymatic inhibitory properties relevant to the management of diabetes mellitus, obesity, and neurodegenerative and skin disorders. A combination of colorimetric assays and ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) were applied for the phytochemical screening of leaf, root, twig, and fruit extracts (methanol and ethyl acetate). In vitro antioxidant evaluations were via radical scavenging abilities (DPPH, ABTS), reducing potential (FRAP, CUPRAC), chelating power, and total antioxidant capacity (phosphomolybdenum). Seven key metabolic enzymes (α-amylase, α-glucosidase, tyrosinase, elastase, lipase, AChE, and BChE) were targeted to determine the inhibitory effects. Multivariate and in silico docking analysis were performed on collected data. Methanolic fruit extract yielded the highest total phenolic, tannin, and triterpenoid contents (174.18 ± 4.27 mg GAE/g, 176.24 ± 3.10 mg CE/g, 63.11 ± 3.27 mg OAE/g, respectively); significantly depressed tyrosinase, elastase, and α-amylase activities (155.35 ± 0.29 mg KAE/g, 4.56 ± 0.10 mg CAE/g, 1.00 ± 0.05 mmol ACAE/g, accordingly); and harboured the most potent antioxidant capacities with DPPH, CUPRAC, FRAP (492.62 ± 5.31, 961.46 ± 11.18, 552.49 ± 8.71 mg TE/g, respectively), and phosphomolybdenum (4.17 ± 0.31 mmol TE/g) assays. Multivariate analysis suggested that the type of solvents used influenced the biological activities more compared to plant parts. Docking analysis showed that azelaic acid binds with tyrosinase by Van der Waals and conventional hydrogen bonds. We anticipate that the present study may establish baseline data on this halophyte that could open new avenues for the development of biomedicine. |
| first_indexed | 2024-03-10T19:59:22Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:59:22Z |
| publishDate | 2020-05-01 |
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| series | Biomolecules |
| spelling | doaj.art-6b8d0d44878b487bab1b755e24fb7bbb2023-11-19T23:42:28ZengMDPI AGBiomolecules2218-273X2020-05-0110573110.3390/biom10050731Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate AnalysisNabeelah Bibi Sadeer0Kouadio Ibrahime Sinan1Zoltán Cziáky2József Jekő3Gokhan Zengin4Rajesh Jeewon5Hassan H. Abdallah6Kannan R. R. Rengasamy7Mohamad Fawzi Mahomoodally8Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, MauritiusDepartment of Biology, Science Faculty, Selcuk University, 42130 Konya, TurkeyAgricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400 Nyíregyháza, HungaryAgricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400 Nyíregyháza, HungaryDepartment of Biology, Science Faculty, Selcuk University, 42130 Konya, TurkeyDepartment of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, MauritiusChemistry Department, College of education, Salahaddin University-Erbil, 44001 Erbil, IraqDepartment of Bioresources and Food Science, College of Life Sciences, Konkuk University, Seoul 05029, KoreaDepartment for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh 758307, Vietnam<i>Bruguiera gymnorhiza</i> (L.) Lam. is claimed to effectively manage a number of ailments including diabetes and associated complications. Nonetheless, no attempt has been made to delineate its pharmacological propensities and phytochemical profile. This study was designed to appraise the antioxidant and enzymatic inhibitory properties relevant to the management of diabetes mellitus, obesity, and neurodegenerative and skin disorders. A combination of colorimetric assays and ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) were applied for the phytochemical screening of leaf, root, twig, and fruit extracts (methanol and ethyl acetate). In vitro antioxidant evaluations were via radical scavenging abilities (DPPH, ABTS), reducing potential (FRAP, CUPRAC), chelating power, and total antioxidant capacity (phosphomolybdenum). Seven key metabolic enzymes (α-amylase, α-glucosidase, tyrosinase, elastase, lipase, AChE, and BChE) were targeted to determine the inhibitory effects. Multivariate and in silico docking analysis were performed on collected data. Methanolic fruit extract yielded the highest total phenolic, tannin, and triterpenoid contents (174.18 ± 4.27 mg GAE/g, 176.24 ± 3.10 mg CE/g, 63.11 ± 3.27 mg OAE/g, respectively); significantly depressed tyrosinase, elastase, and α-amylase activities (155.35 ± 0.29 mg KAE/g, 4.56 ± 0.10 mg CAE/g, 1.00 ± 0.05 mmol ACAE/g, accordingly); and harboured the most potent antioxidant capacities with DPPH, CUPRAC, FRAP (492.62 ± 5.31, 961.46 ± 11.18, 552.49 ± 8.71 mg TE/g, respectively), and phosphomolybdenum (4.17 ± 0.31 mmol TE/g) assays. Multivariate analysis suggested that the type of solvents used influenced the biological activities more compared to plant parts. Docking analysis showed that azelaic acid binds with tyrosinase by Van der Waals and conventional hydrogen bonds. We anticipate that the present study may establish baseline data on this halophyte that could open new avenues for the development of biomedicine.https://www.mdpi.com/2218-273X/10/5/731mangroveMauritiusmultivariate analysisphytochemicalsenzymatic effectsdocking |
| spellingShingle | Nabeelah Bibi Sadeer Kouadio Ibrahime Sinan Zoltán Cziáky József Jekő Gokhan Zengin Rajesh Jeewon Hassan H. Abdallah Kannan R. R. Rengasamy Mohamad Fawzi Mahomoodally Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis Biomolecules mangrove Mauritius multivariate analysis phytochemicals enzymatic effects docking |
| title | Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis |
| title_full | Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis |
| title_fullStr | Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis |
| title_full_unstemmed | Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis |
| title_short | Assessment of the Pharmacological Properties and Phytochemical Profile of <i>Bruguiera gymnorhiza</i> (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis |
| title_sort | assessment of the pharmacological properties and phytochemical profile of i bruguiera gymnorhiza i l lam using in vitro studies in silico docking and multivariate analysis |
| topic | mangrove Mauritius multivariate analysis phytochemicals enzymatic effects docking |
| url | https://www.mdpi.com/2218-273X/10/5/731 |
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