Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L.
Pulses are a key source of dietary proteins in human nutrition. Despite several efforts to increase the production, various constraints, such as biotic and abiotic factors, threaten pulse production by various means. Bruchids (<i>Callosobruchus</i> spp.) are the serious issue of concern,...
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MDPI AG
2023-05-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/6/781 |
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| author | Prince Sahu Mahendra Singh Rakesh Pandey Mukesh Kumar Mishra Akhilesh Kumar Singh Bhupendra Kumar Singh Surendra Kumar Singh Ashutosh Rai Vishal Chugh Gaurav Shukla Saurabh Singh Kartikey Singh Mukul Kumar Chandra Mohan Singh |
| author_facet | Prince Sahu Mahendra Singh Rakesh Pandey Mukesh Kumar Mishra Akhilesh Kumar Singh Bhupendra Kumar Singh Surendra Kumar Singh Ashutosh Rai Vishal Chugh Gaurav Shukla Saurabh Singh Kartikey Singh Mukul Kumar Chandra Mohan Singh |
| author_sort | Prince Sahu |
| collection | DOAJ |
| description | Pulses are a key source of dietary proteins in human nutrition. Despite several efforts to increase the production, various constraints, such as biotic and abiotic factors, threaten pulse production by various means. Bruchids (<i>Callosobruchus</i> spp.) are the serious issue of concern, particularly in storage conditions. Understanding host–plant resistance at morphological, biochemical and molecular levels is the best way to minimize yield losses. The 117 mungbean (<i>Vigna radiata</i> L. Wilczek) genotypes, including endemic wild relatives, were screened for resistance against <i>Callosobruchus chinensis</i>; among them, two genotypes, PRR 2008-2 and PRR 2008-2-sel, which belong to <i>V. umbellata</i> (Thumb.), were identified as highly resistant. The expression of antioxidants in susceptible and resistant genotypes revealed that the activity of phenylalanine ammonia lyase (PAL) was upregulated in the highly resistant wild <i>Vigna</i> species and lower in the cultivated susceptible genotypes, along with other biomarkers. Further, the SCoT-based genotyping revealed SCoT-30 (200 bp), SCoT-31 (1200 bp) and SCoT-32 (300 bp) as unique amplicons, which might be useful for developing the novel ricebean-based SCAR markers to accelerate the molecular breeding programme. |
| first_indexed | 2024-03-11T02:45:42Z |
| format | Article |
| id | doaj.art-6b8f10e90ff34b8e9162a2100b38865e |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T02:45:42Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-6b8f10e90ff34b8e9162a2100b38865e2023-11-18T09:22:29ZengMDPI AGBiology2079-77372023-05-0112678110.3390/biology12060781Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L.Prince Sahu0Mahendra Singh1Rakesh Pandey2Mukesh Kumar Mishra3Akhilesh Kumar Singh4Bhupendra Kumar Singh5Surendra Kumar Singh6Ashutosh Rai7Vishal Chugh8Gaurav Shukla9Saurabh Singh10Kartikey Singh11Mukul Kumar12Chandra Mohan Singh13Department of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Plant Protection, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Basic and Social Sciences, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Basic and Social Sciences, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Statistics and Computer Science, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Entomology, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda 210 001, IndiaDepartment of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda 210 001, IndiaPulses are a key source of dietary proteins in human nutrition. Despite several efforts to increase the production, various constraints, such as biotic and abiotic factors, threaten pulse production by various means. Bruchids (<i>Callosobruchus</i> spp.) are the serious issue of concern, particularly in storage conditions. Understanding host–plant resistance at morphological, biochemical and molecular levels is the best way to minimize yield losses. The 117 mungbean (<i>Vigna radiata</i> L. Wilczek) genotypes, including endemic wild relatives, were screened for resistance against <i>Callosobruchus chinensis</i>; among them, two genotypes, PRR 2008-2 and PRR 2008-2-sel, which belong to <i>V. umbellata</i> (Thumb.), were identified as highly resistant. The expression of antioxidants in susceptible and resistant genotypes revealed that the activity of phenylalanine ammonia lyase (PAL) was upregulated in the highly resistant wild <i>Vigna</i> species and lower in the cultivated susceptible genotypes, along with other biomarkers. Further, the SCoT-based genotyping revealed SCoT-30 (200 bp), SCoT-31 (1200 bp) and SCoT-32 (300 bp) as unique amplicons, which might be useful for developing the novel ricebean-based SCAR markers to accelerate the molecular breeding programme.https://www.mdpi.com/2079-7737/12/6/781<i>Callosobruchus chinensis</i>mungbeanCWRPALSOD<i>Vigna radiata</i> |
| spellingShingle | Prince Sahu Mahendra Singh Rakesh Pandey Mukesh Kumar Mishra Akhilesh Kumar Singh Bhupendra Kumar Singh Surendra Kumar Singh Ashutosh Rai Vishal Chugh Gaurav Shukla Saurabh Singh Kartikey Singh Mukul Kumar Chandra Mohan Singh Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L. Biology <i>Callosobruchus chinensis</i> mungbean CWR PAL SOD <i>Vigna radiata</i> |
| title | Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L. |
| title_full | Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L. |
| title_fullStr | Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L. |
| title_full_unstemmed | Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L. |
| title_short | Screening of Comprehensive Panel of Cultivated and Wild <i>Vigna</i> Species for Resistance to Pulse Beetle, <i>Callosobruchus chinensis</i> L. |
| title_sort | screening of comprehensive panel of cultivated and wild i vigna i species for resistance to pulse beetle i callosobruchus chinensis i l |
| topic | <i>Callosobruchus chinensis</i> mungbean CWR PAL SOD <i>Vigna radiata</i> |
| url | https://www.mdpi.com/2079-7737/12/6/781 |
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