Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A
Motor and non-motor crosslinking proteins play critical roles in determining the size and stability of microtubule-based architectures. Currently, we have a limited understanding of how geometrical properties of microtubule arrays, in turn, regulate the output of crosslinking proteins. Here we inves...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/32595 |
| _version_ | 1818024140493291520 |
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| author | Sithara Wijeratne Radhika Subramanian |
| author_facet | Sithara Wijeratne Radhika Subramanian |
| author_sort | Sithara Wijeratne |
| collection | DOAJ |
| description | Motor and non-motor crosslinking proteins play critical roles in determining the size and stability of microtubule-based architectures. Currently, we have a limited understanding of how geometrical properties of microtubule arrays, in turn, regulate the output of crosslinking proteins. Here we investigate this problem in the context of microtubule sliding by two interacting proteins: the non-motor crosslinker PRC1 and the kinesin Kif4A. The collective activity of PRC1 and Kif4A also results in their accumulation at microtubule plus-ends (‘end-tag’). Sliding stalls when the end-tags on antiparallel microtubules collide, forming a stable overlap. Interestingly, we find that structural properties of the initial array regulate microtubule organization by PRC1-Kif4A. First, sliding velocity scales with initial microtubule-overlap length. Second, the width of the final overlap scales with microtubule lengths. Our analyses reveal how micron-scale geometrical features of antiparallel microtubules can regulate the activity of nanometer-sized proteins to define the structure and mechanics of microtubule-based architectures. |
| first_indexed | 2024-12-10T03:55:29Z |
| format | Article |
| id | doaj.art-6b8f7cb5bcca4c66b3a03bf75028a899 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:55:29Z |
| publishDate | 2018-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-6b8f7cb5bcca4c66b3a03bf75028a8992022-12-22T02:03:08ZengeLife Sciences Publications LtdeLife2050-084X2018-10-01710.7554/eLife.32595Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4ASithara Wijeratne0https://orcid.org/0000-0002-3935-926XRadhika Subramanian1https://orcid.org/0000-0002-3011-9403Department of Molecular Biology, Massachusetts General Hospital, Boston, United States; Department of Genetics, Harvard Medical School, Boston, United StatesDepartment of Molecular Biology, Massachusetts General Hospital, Boston, United States; Department of Genetics, Harvard Medical School, Boston, United StatesMotor and non-motor crosslinking proteins play critical roles in determining the size and stability of microtubule-based architectures. Currently, we have a limited understanding of how geometrical properties of microtubule arrays, in turn, regulate the output of crosslinking proteins. Here we investigate this problem in the context of microtubule sliding by two interacting proteins: the non-motor crosslinker PRC1 and the kinesin Kif4A. The collective activity of PRC1 and Kif4A also results in their accumulation at microtubule plus-ends (‘end-tag’). Sliding stalls when the end-tags on antiparallel microtubules collide, forming a stable overlap. Interestingly, we find that structural properties of the initial array regulate microtubule organization by PRC1-Kif4A. First, sliding velocity scales with initial microtubule-overlap length. Second, the width of the final overlap scales with microtubule lengths. Our analyses reveal how micron-scale geometrical features of antiparallel microtubules can regulate the activity of nanometer-sized proteins to define the structure and mechanics of microtubule-based architectures.https://elifesciences.org/articles/32595microtubule organizationmotor proteinmicrotubule sliding |
| spellingShingle | Sithara Wijeratne Radhika Subramanian Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A eLife microtubule organization motor protein microtubule sliding |
| title | Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A |
| title_full | Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A |
| title_fullStr | Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A |
| title_full_unstemmed | Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A |
| title_short | Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A |
| title_sort | geometry of antiparallel microtubule bundles regulates relative sliding and stalling by prc1 and kif4a |
| topic | microtubule organization motor protein microtubule sliding |
| url | https://elifesciences.org/articles/32595 |
| work_keys_str_mv | AT sitharawijeratne geometryofantiparallelmicrotubulebundlesregulatesrelativeslidingandstallingbyprc1andkif4a AT radhikasubramanian geometryofantiparallelmicrotubulebundlesregulatesrelativeslidingandstallingbyprc1andkif4a |