Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE
Background & Objective: Asparaginase enzymes are effective in treating lymphoblastic leukemia cancer. This enzyme is isolated from bacterial sources and commercially available as an anticancer drug. The aim of this study was to isolate and purifiy L-asparaginase from persian gulf Streptomyces is...
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2020-08-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-2192-en.pdf |
| _version_ | 1827812290354741248 |
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| author | Hadiseh Soofer Kumarss Amini Seyed Mohammad Masoud Shushtarian |
| author_facet | Hadiseh Soofer Kumarss Amini Seyed Mohammad Masoud Shushtarian |
| author_sort | Hadiseh Soofer |
| collection | DOAJ |
| description | Background & Objective: Asparaginase enzymes are effective in treating lymphoblastic leukemia cancer. This enzyme is isolated from bacterial sources and commercially available as an anticancer drug. The aim of this study was to isolate and purifiy L-asparaginase from persian gulf Streptomyces isolates and evaluate its cloned gene expression in E. coli by Real-Time and SDS-PAGE.
Materials & methods: Streptomyces isolates were isolated from the Persian Gulf and identified by biochemical tests, then, L-asparaginase genes were isolated from streptomyces by PCR method. The amplified fragment was entered into vector pTG19 by the TA cloning technique. In the next step, the recombinant vector was transformed into E. coli using CaCl2 method and cloning confirmation using conventional methods.
Result: The results of this study showed that Streptomyces isolates from the Persian Gulf were capable of producing L-asparaginase. Eventually, the gene of this enzyme was successfully transformed to E. coli by vector and produced high efficiency L-asparaginase.
Conclusion: Finding new bacterial strains producing L-asparaginase and using gene engineering techniques can lead to better performance of these enzymes, thereby taking a big step in the direction of increasing and facilitating the production of L-asparaginase in the pharmaceutical industry. |
| first_indexed | 2024-03-11T23:16:33Z |
| format | Article |
| id | doaj.art-6b8fe1ad458f43769d254b61b37e69f3 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:16:33Z |
| publishDate | 2020-08-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-6b8fe1ad458f43769d254b61b37e69f32023-09-20T21:27:39ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232020-08-0110325582566Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGEHadiseh Soofer0Kumarss Amini1Seyed Mohammad Masoud Shushtarian2 Department of Biochemistry, Faculty of Advanced Science and Technology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic azad university, Saveh, Iran Department of Biophysics & Biochemistry, Faculty of Advanced Science and Technology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran Background & Objective: Asparaginase enzymes are effective in treating lymphoblastic leukemia cancer. This enzyme is isolated from bacterial sources and commercially available as an anticancer drug. The aim of this study was to isolate and purifiy L-asparaginase from persian gulf Streptomyces isolates and evaluate its cloned gene expression in E. coli by Real-Time and SDS-PAGE. Materials & methods: Streptomyces isolates were isolated from the Persian Gulf and identified by biochemical tests, then, L-asparaginase genes were isolated from streptomyces by PCR method. The amplified fragment was entered into vector pTG19 by the TA cloning technique. In the next step, the recombinant vector was transformed into E. coli using CaCl2 method and cloning confirmation using conventional methods. Result: The results of this study showed that Streptomyces isolates from the Persian Gulf were capable of producing L-asparaginase. Eventually, the gene of this enzyme was successfully transformed to E. coli by vector and produced high efficiency L-asparaginase. Conclusion: Finding new bacterial strains producing L-asparaginase and using gene engineering techniques can lead to better performance of these enzymes, thereby taking a big step in the direction of increasing and facilitating the production of L-asparaginase in the pharmaceutical industry.http://jabs.fums.ac.ir/article-1-2192-en.pdfl-asparaginasestreptomyceslymphoblastic leukemia |
| spellingShingle | Hadiseh Soofer Kumarss Amini Seyed Mohammad Masoud Shushtarian Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE Journal of Advanced Biomedical Sciences l-asparaginase streptomyces lymphoblastic leukemia |
| title | Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE |
| title_full | Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE |
| title_fullStr | Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE |
| title_full_unstemmed | Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE |
| title_short | Isolation and Purification of L-Asparaginase from Persian Gulf Streptomyces Isolates and Evaluation of It’s Cloned Gene Expression in Escherichia Coli by Real-Time and SDS-PAGE |
| title_sort | isolation and purification of l asparaginase from persian gulf streptomyces isolates and evaluation of it s cloned gene expression in escherichia coli by real time and sds page |
| topic | l-asparaginase streptomyces lymphoblastic leukemia |
| url | http://jabs.fums.ac.ir/article-1-2192-en.pdf |
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