The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells
Objective(s):Osteoarthritis (OA) is globally one of the most common diseases from the middle age onwards. Cartilage is an avascular tissue therefore it cannot be repaired in the body. Conservative treatments have failed as a good remedy and cell therapy as a decisive cure is needed. One of the best...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2014-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_3188_78752e5fe5016481a58b91f517317c0f.html |
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| author | Ebrahim Esfandiari Shiva Roshankhah Mohammad Mardani Batool Hashemibeni Erfan Naghsh Mohammad Kazemi Mohammadreza Salahshoor |
| author_facet | Ebrahim Esfandiari Shiva Roshankhah Mohammad Mardani Batool Hashemibeni Erfan Naghsh Mohammad Kazemi Mohammadreza Salahshoor |
| author_sort | Ebrahim Esfandiari |
| collection | DOAJ |
| description | Objective(s):Osteoarthritis (OA) is globally one of the most common diseases from the middle age onwards. Cartilage is an avascular tissue therefore it cannot be repaired in the body. Conservative treatments have failed as a good remedy and cell therapy as a decisive cure is needed. One of the best and easily accessible cell sources for this purpose is adipose-derived stem cells which can be differentiated into chondrocytes by tissue engineering techniques. Chemical and physical inducers have a key role in stem cell – chondrocyte differentiation. We have tried to determine the role of electric fields (EF) in promoting this kind of chondrogenesis process.
Materials and Methods: Human adipose derived stem cells (ADSCs) were extracted from subcutaneous abdominal adipose tissue during cesarean section. A high frequency (60 KHz) EF (20 mv/cm), as a physical inducer for chondrogenesis in a 3D micromass culture system of ADSCs was utilized. Also, MTT, ELISA, flow cytometry, and real-time PCR techniques were used for this study.
Results: We found that using physical electric fields leads to chondrogenesis. Furthermore, results show that using both physical (EF) and chemical (TGFβ3) inducers simultaneously, has best outcomes in chondrogenesis, and expression of SOX9 andtype II collagen genes. It also causes significant decreased expression of type I and X collagen genes in pure EF group compared with control group.
Conclusion:The EF was found as a proper effective inducer in chondrogenic differentiation of human ADSCs micromass culture. |
| first_indexed | 2024-04-13T02:03:56Z |
| format | Article |
| id | doaj.art-6b91c2e1c9da4ed18dcfe18b2090291c |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T02:03:56Z |
| publishDate | 2014-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-6b91c2e1c9da4ed18dcfe18b2090291c2022-12-22T03:07:32ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742014-08-011785715763188The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cellsEbrahim Esfandiari0Shiva Roshankhah1Mohammad Mardani2Batool Hashemibeni3Erfan Naghsh4Mohammad Kazemi5Mohammadreza Salahshoor6Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Electrical Engineering, Engineering School, Isfahan University, Isfahan, IranDepartment of Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, IranObjective(s):Osteoarthritis (OA) is globally one of the most common diseases from the middle age onwards. Cartilage is an avascular tissue therefore it cannot be repaired in the body. Conservative treatments have failed as a good remedy and cell therapy as a decisive cure is needed. One of the best and easily accessible cell sources for this purpose is adipose-derived stem cells which can be differentiated into chondrocytes by tissue engineering techniques. Chemical and physical inducers have a key role in stem cell – chondrocyte differentiation. We have tried to determine the role of electric fields (EF) in promoting this kind of chondrogenesis process. Materials and Methods: Human adipose derived stem cells (ADSCs) were extracted from subcutaneous abdominal adipose tissue during cesarean section. A high frequency (60 KHz) EF (20 mv/cm), as a physical inducer for chondrogenesis in a 3D micromass culture system of ADSCs was utilized. Also, MTT, ELISA, flow cytometry, and real-time PCR techniques were used for this study. Results: We found that using physical electric fields leads to chondrogenesis. Furthermore, results show that using both physical (EF) and chemical (TGFβ3) inducers simultaneously, has best outcomes in chondrogenesis, and expression of SOX9 andtype II collagen genes. It also causes significant decreased expression of type I and X collagen genes in pure EF group compared with control group. Conclusion:The EF was found as a proper effective inducer in chondrogenic differentiation of human ADSCs micromass culture.http://ijbms.mums.ac.ir/pdf_3188_78752e5fe5016481a58b91f517317c0f.htmlADSCs Chondrogenesis High frequency electric field |
| spellingShingle | Ebrahim Esfandiari Shiva Roshankhah Mohammad Mardani Batool Hashemibeni Erfan Naghsh Mohammad Kazemi Mohammadreza Salahshoor The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells Iranian Journal of Basic Medical Sciences ADSCs Chondrogenesis High frequency electric field |
| title | The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells |
| title_full | The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells |
| title_fullStr | The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells |
| title_full_unstemmed | The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells |
| title_short | The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells |
| title_sort | effect of high frequency electric field on enhancement of chondrogenesis in human adipose derived stem cells |
| topic | ADSCs Chondrogenesis High frequency electric field |
| url | http://ijbms.mums.ac.ir/pdf_3188_78752e5fe5016481a58b91f517317c0f.html |
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