Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy
Here, the light-induced changes of the core antenna complex chlorophyll protein 43 (CP43) isolated from PSII of spinach were assessed using infrared and terahertz (THz) spectroscopy. We found that the collective vibrational modes at low frequencies and the secondary structures of CP43 were altered u...
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| Format: | Article |
| Language: | English |
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Academy of Sciences of the Czech Republic, Institute of Experimental Botany
2020-06-01
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| Series: | Photosynthetica |
| Subjects: | |
| Online Access: | https://ps.ueb.cas.cz/artkey/phs-202003-0009_light-stability-of-the-isolated-cp43-studied-by-infrared-and-terahertz-spectroscopy.php |
| _version_ | 1818526567183155200 |
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| author | Y.G. QU X.L. WANG T.Y. KUANG |
| author_facet | Y.G. QU X.L. WANG T.Y. KUANG |
| author_sort | Y.G. QU |
| collection | DOAJ |
| description | Here, the light-induced changes of the core antenna complex chlorophyll protein 43 (CP43) isolated from PSII of spinach were assessed using infrared and terahertz (THz) spectroscopy. We found that the collective vibrational modes at low frequencies and the secondary structures of CP43 were altered upon illumination. The helix changed more than any other secondary structure. CP43 is more easily damaged by illumination than PSII. Infrared and THz spectroscopy have different functions in protein studies. THz spectroscopy has potential in evaluating the stability of proteins. |
| first_indexed | 2024-12-11T06:24:41Z |
| format | Article |
| id | doaj.art-6b9576ec78ef475f8704070878dc8db5 |
| institution | Directory Open Access Journal |
| issn | 0300-3604 1573-9058 |
| language | English |
| last_indexed | 2024-12-11T06:24:41Z |
| publishDate | 2020-06-01 |
| publisher | Academy of Sciences of the Czech Republic, Institute of Experimental Botany |
| record_format | Article |
| series | Photosynthetica |
| spelling | doaj.art-6b9576ec78ef475f8704070878dc8db52022-12-22T01:17:42ZengAcademy of Sciences of the Czech Republic, Institute of Experimental BotanyPhotosynthetica0300-36041573-90582020-06-0158375576110.32615/ps.2020.019phs-202003-0009Light stability of the isolated CP43 studied by infrared and terahertz spectroscopyY.G. QU0X.L. WANG1T.Y. KUANG2College of Life Sciences, Linyi University, 276000 Linyi, ChinaCollege of Life Sciences, Shandong Agricultural University, 271018 Taian, ChinaKey Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, ChinaHere, the light-induced changes of the core antenna complex chlorophyll protein 43 (CP43) isolated from PSII of spinach were assessed using infrared and terahertz (THz) spectroscopy. We found that the collective vibrational modes at low frequencies and the secondary structures of CP43 were altered upon illumination. The helix changed more than any other secondary structure. CP43 is more easily damaged by illumination than PSII. Infrared and THz spectroscopy have different functions in protein studies. THz spectroscopy has potential in evaluating the stability of proteins.https://ps.ueb.cas.cz/artkey/phs-202003-0009_light-stability-of-the-isolated-cp43-studied-by-infrared-and-terahertz-spectroscopy.phpamide i bandconformationdegradationphotoinhibition |
| spellingShingle | Y.G. QU X.L. WANG T.Y. KUANG Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy Photosynthetica amide i band conformation degradation photoinhibition |
| title | Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy |
| title_full | Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy |
| title_fullStr | Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy |
| title_full_unstemmed | Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy |
| title_short | Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy |
| title_sort | light stability of the isolated cp43 studied by infrared and terahertz spectroscopy |
| topic | amide i band conformation degradation photoinhibition |
| url | https://ps.ueb.cas.cz/artkey/phs-202003-0009_light-stability-of-the-isolated-cp43-studied-by-infrared-and-terahertz-spectroscopy.php |
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